The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics
<p><strong><em>Introduction</em></strong><strong>: </strong>Due to more resistance of pathogenic bacteria to new and current antibiotics researchers are looking to find the agents of herbal with antimicrobial activities in order to repl...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-11-01
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| Series: | Journal of Medical Bacteriology |
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| Online Access: | http://jmb.tums.ac.ir/index.php/jmb/article/view/65 |
| _version_ | 1818561935376908288 |
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| author | Mohammad Bokaeian BaratAli Fakheri Nafise Mahdi-Nejad Mehdi Hassansahian Saeide Saeidi |
| author_facet | Mohammad Bokaeian BaratAli Fakheri Nafise Mahdi-Nejad Mehdi Hassansahian Saeide Saeidi |
| author_sort | Mohammad Bokaeian |
| collection | DOAJ |
| description | <p><strong><em>Introduction</em></strong><strong>: </strong>Due to more resistance of pathogenic bacteria to new and current antibiotics researchers are looking to find the agents of herbal with antimicrobial activities in order to replace chemical drugs.</p><p><strong><em>M</em></strong><strong><em>e</em></strong><strong><em>thods</em></strong><strong>: </strong>The herbal extract of <em>Withani somnifera </em>was done by using a rotary vacuum,20 strains of <em>Pseudomons aeruginosa </em>were isolated from urinary infections hospitalized patients in city of Zabol hospital. The MIC <em>Withani somnifera </em>were determined by dilution method in various concentrations. Sensitivity of strains to multiple antibiotics was evaluated by standard disk diffusion Kirby-Bauer.</p><p><strong><em>Results</em></strong><strong>: </strong>The result showed that <em>P. aeruginosa </em>were resistance to 4 of the agents including ampicillin (85%), nitrofurantoin (65%), nalidixic acid (65%), ciprofloxacin (15%) and for 5 strains of Pseudomonas showed MIC with activity of 100 ppm.</p><p><strong><em>Conclusion</em>: </strong>This study has suggested the effect of winter cherry extract on <em>P. aeruginosa </em>in the in vitro assay. It s effectiveness of on in vivo system can be examined in future.</p> |
| first_indexed | 2024-12-14T00:57:12Z |
| format | Article |
| id | doaj.art-31099a8333f146b68c9051ec19695ccf |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-12-14T00:57:12Z |
| publishDate | 2015-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-31099a8333f146b68c9051ec19695ccf2022-12-21T23:23:29ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-11-0141-2535758The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective AntibioticsMohammad Bokaeian0BaratAli Fakheri1Nafise Mahdi-Nejad2Mehdi Hassansahian3Saeide Saeidi4Infectious Disease and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR IranDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, IR IranDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, IR IranDepartments of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, IR IranInfectious Disease and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran<p><strong><em>Introduction</em></strong><strong>: </strong>Due to more resistance of pathogenic bacteria to new and current antibiotics researchers are looking to find the agents of herbal with antimicrobial activities in order to replace chemical drugs.</p><p><strong><em>M</em></strong><strong><em>e</em></strong><strong><em>thods</em></strong><strong>: </strong>The herbal extract of <em>Withani somnifera </em>was done by using a rotary vacuum,20 strains of <em>Pseudomons aeruginosa </em>were isolated from urinary infections hospitalized patients in city of Zabol hospital. The MIC <em>Withani somnifera </em>were determined by dilution method in various concentrations. Sensitivity of strains to multiple antibiotics was evaluated by standard disk diffusion Kirby-Bauer.</p><p><strong><em>Results</em></strong><strong>: </strong>The result showed that <em>P. aeruginosa </em>were resistance to 4 of the agents including ampicillin (85%), nitrofurantoin (65%), nalidixic acid (65%), ciprofloxacin (15%) and for 5 strains of Pseudomonas showed MIC with activity of 100 ppm.</p><p><strong><em>Conclusion</em>: </strong>This study has suggested the effect of winter cherry extract on <em>P. aeruginosa </em>in the in vitro assay. It s effectiveness of on in vivo system can be examined in future.</p>http://jmb.tums.ac.ir/index.php/jmb/article/view/65Pseudomonas aeruginosa,Antibiotic resistance,Herbal extract, Winter cherry, Antimicrobial activity |
| spellingShingle | Mohammad Bokaeian BaratAli Fakheri Nafise Mahdi-Nejad Mehdi Hassansahian Saeide Saeidi The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics Journal of Medical Bacteriology Pseudomonas aeruginosa,Antibiotic resistance,Herbal extract, Winter cherry, Antimicrobial activity |
| title | The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics |
| title_full | The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics |
| title_fullStr | The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics |
| title_full_unstemmed | The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics |
| title_short | The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics |
| title_sort | survey of withani somnifera extraction against resistant strains of pseudomonas aeruginosa bacteria to selective antibiotics |
| topic | Pseudomonas aeruginosa,Antibiotic resistance,Herbal extract, Winter cherry, Antimicrobial activity |
| url | http://jmb.tums.ac.ir/index.php/jmb/article/view/65 |
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