Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction
PurposeTo explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing.MethodsA total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.1045990/full |
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| author | Dalan Jing Dalan Jing Xiaodan Jiang Xiaodan Jiang Xiaotong Ren Xiaotong Ren Jie Su Jie Su Chen Huang Chen Huang Chen Huang Jiarui Yang Jiarui Yang Ran Hao Xuemin Li Xuemin Li |
| author_facet | Dalan Jing Dalan Jing Xiaodan Jiang Xiaodan Jiang Xiaotong Ren Xiaotong Ren Jie Su Jie Su Chen Huang Chen Huang Chen Huang Jiarui Yang Jiarui Yang Ran Hao Xuemin Li Xuemin Li |
| author_sort | Dalan Jing |
| collection | DOAJ |
| description | PurposeTo explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing.MethodsA total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of dry eye were assessed, and bacterial samples in the conjunctival sac (CS) and meibomian gland (MG) secretions were then identified by bacterial culture identification and metagenomic nanopore sequencing.ResultsThe positive rate of CS bacterial culture in the MGD group was significantly higher than that in the normal group. A more complex composition of bacterial genera was detected in the mild and moderate MGD groups than in the control. However, the severe MGD groups had the simplest composition of bacteria. Metagenomic nanopore sequencing detected more species of bacteria than traditional culture.ConclusionThe CS and MG of MGD patients may have different degrees of bacterial microbiota imbalance. Metagenomic nanopore sequencing technology provides a new way for us to understand the composition of “real-world” ocular surface microorganisms. |
| first_indexed | 2024-04-11T17:30:54Z |
| format | Article |
| id | doaj.art-310a2b6d424b4dd09803282987107bee |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-11T17:30:54Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj.art-310a2b6d424b4dd09803282987107bee2022-12-22T04:12:01ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-11-01910.3389/fmed.2022.10459901045990Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunctionDalan Jing0Dalan Jing1Xiaodan Jiang2Xiaodan Jiang3Xiaotong Ren4Xiaotong Ren5Jie Su6Jie Su7Chen Huang8Chen Huang9Chen Huang10Jiarui Yang11Jiarui Yang12Ran Hao13Xuemin Li14Xuemin Li15Department of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaMedical Research Center, Peking University Third Hospital, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaBeijing Ophthalmology and Visual Sciences Key Laboratory, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Ophthalmology, Peking University Third Hospital, Beijing, ChinaBeijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, ChinaPurposeTo explore the composition of the ocular microbiome in patients with Meibomian gland dysfunction (MGD) using metagenomic nanopore sequencing.MethodsA total of 98 participants were recruited from September to December 2021, including 86 patients with MGD and 12 controls. Symptoms and signs of dry eye were assessed, and bacterial samples in the conjunctival sac (CS) and meibomian gland (MG) secretions were then identified by bacterial culture identification and metagenomic nanopore sequencing.ResultsThe positive rate of CS bacterial culture in the MGD group was significantly higher than that in the normal group. A more complex composition of bacterial genera was detected in the mild and moderate MGD groups than in the control. However, the severe MGD groups had the simplest composition of bacteria. Metagenomic nanopore sequencing detected more species of bacteria than traditional culture.ConclusionThe CS and MG of MGD patients may have different degrees of bacterial microbiota imbalance. Metagenomic nanopore sequencing technology provides a new way for us to understand the composition of “real-world” ocular surface microorganisms.https://www.frontiersin.org/articles/10.3389/fmed.2022.1045990/fullmetagenomic nanopore sequencingmeibomian gland dysfunctionmicrobiomebacterial floramicrobiota imbalance |
| spellingShingle | Dalan Jing Dalan Jing Xiaodan Jiang Xiaodan Jiang Xiaotong Ren Xiaotong Ren Jie Su Jie Su Chen Huang Chen Huang Chen Huang Jiarui Yang Jiarui Yang Ran Hao Xuemin Li Xuemin Li Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction Frontiers in Medicine metagenomic nanopore sequencing meibomian gland dysfunction microbiome bacterial flora microbiota imbalance |
| title | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
| title_full | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
| title_fullStr | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
| title_full_unstemmed | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
| title_short | Metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
| title_sort | metagenomic nanopore sequencing of ocular microbiome in patients with meibomian gland dysfunction |
| topic | metagenomic nanopore sequencing meibomian gland dysfunction microbiome bacterial flora microbiota imbalance |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.1045990/full |
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