Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
A 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-promoting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress resp...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/11/3118 |
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| author | Kumbukani Mzengereza Manabu Ishikawa Shunsuke Koshio Saichiro Yokoyama Zhang Yukun Ronick S. Shadrack Seok Seo Tomonari Kotani Serge Dossou Mohammed F. El Basuini Mahmoud A. O. Dawood |
| author_facet | Kumbukani Mzengereza Manabu Ishikawa Shunsuke Koshio Saichiro Yokoyama Zhang Yukun Ronick S. Shadrack Seok Seo Tomonari Kotani Serge Dossou Mohammed F. El Basuini Mahmoud A. O. Dawood |
| author_sort | Kumbukani Mzengereza |
| collection | DOAJ |
| description | A 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-promoting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress response, non-specific immunity of <i>Pagrus major</i>. Four isonitrogenous (490.7 g/kg of crude protein) and isolipidic (91.5 g/kg total lipid) experimental diets were formulated and designated as camelina meal (CM0), soyabean meal (SBM20.5), CM20.5, and CM33 based on protein contents. At the end of the feed trial, significantly higher (<i>p</i> < 0.05) weight gain, specific growth rate, and feed intake but lower feed conversion ratio were recorded in fish fed CM0, SBM20.5, and CM20.5 than fish fed CM33. The lowest growth, feed utilization, enzyme activity, and digestibility were recorded in fish fed CM33. Significantly higher pepsin, amylase, and protease activities were observed in fish fed CM0, SBM20.5, and CM20.5 diets than fish fed CM33. The highest ADC of protein was recorded in fish fed CM0, SBM20.5, and CM20.5 diets. Hematocrit levels were depressed CM33 while total serum protein, total cholesterol, triglyceride, blood urea nitrogen, total bilirubin, aspartate aminotransferase, and alanine aminotransferase were not significantly changed by the inclusion of CM. Non-specific immune variables (lysozyme activity, peroxidase activity in serum and nitro blue tetrazolium) in fish fed CM0, SBM20.5, and CM20.5 were significantly higher than in fish fed CM33 diet. The superoxide dismutase of fish fed CM20.5 was not significantly different from CM0 and SBM20.5 (<i>p</i> > 0.05). Catalase and low salinity stress test show that CM0, SBM20.5, and CM20.5 were not significantly (<i>p</i> > 0.05) different, while CM33 was significantly lower than the rest of the diets. TBARs show that CM20.5 and CM33 diets were significantly different (<i>p</i> < 0.05), but CM20.5 was not significantly different from SBM20.5. Significantly higher hepatic <i>IGF-1</i> and <i>IGF-2</i> mRNA expression was found in fish-fed diet groups CM0, SBM20.5, and CM20.5 than fish fed CM33. The present study indicated that the addition of CM up 205 kg/kg to diet maintains growth, digestive enzymes, nutrient digestibility, immunity, stress resistance, and feed utilization efficiency of red sea bream. |
| first_indexed | 2024-03-09T04:35:39Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T04:35:39Z |
| publishDate | 2021-10-01 |
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| series | Animals |
| spelling | doaj.art-310cafe84dc14377961888c708a6e9a82023-12-03T13:27:48ZengMDPI AGAnimals2076-26152021-10-011111311810.3390/ani11113118Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)Kumbukani Mzengereza0Manabu Ishikawa1Shunsuke Koshio2Saichiro Yokoyama3Zhang Yukun4Ronick S. Shadrack5Seok Seo6Tomonari Kotani7Serge Dossou8Mohammed F. El Basuini9Mahmoud A. O. Dawood10The United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, JapanLaboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, JapanLaboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, JapanLaboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, JapanThe United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, JapanThe United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, JapanThe United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, JapanLaboratory of Larval Rearing Management, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, JapanLaboratoire d’Hydrobiologie et Aquaculture, Faculté des Sciences Agronomies’, Université d’Abomey Calavi, Cotonou 01 BP:526, BeninFaculty of Desert Agriculture, King Salman International University, South Sinai 46612, EgyptDepartment of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, EgyptA 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-promoting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress response, non-specific immunity of <i>Pagrus major</i>. Four isonitrogenous (490.7 g/kg of crude protein) and isolipidic (91.5 g/kg total lipid) experimental diets were formulated and designated as camelina meal (CM0), soyabean meal (SBM20.5), CM20.5, and CM33 based on protein contents. At the end of the feed trial, significantly higher (<i>p</i> < 0.05) weight gain, specific growth rate, and feed intake but lower feed conversion ratio were recorded in fish fed CM0, SBM20.5, and CM20.5 than fish fed CM33. The lowest growth, feed utilization, enzyme activity, and digestibility were recorded in fish fed CM33. Significantly higher pepsin, amylase, and protease activities were observed in fish fed CM0, SBM20.5, and CM20.5 diets than fish fed CM33. The highest ADC of protein was recorded in fish fed CM0, SBM20.5, and CM20.5 diets. Hematocrit levels were depressed CM33 while total serum protein, total cholesterol, triglyceride, blood urea nitrogen, total bilirubin, aspartate aminotransferase, and alanine aminotransferase were not significantly changed by the inclusion of CM. Non-specific immune variables (lysozyme activity, peroxidase activity in serum and nitro blue tetrazolium) in fish fed CM0, SBM20.5, and CM20.5 were significantly higher than in fish fed CM33 diet. The superoxide dismutase of fish fed CM20.5 was not significantly different from CM0 and SBM20.5 (<i>p</i> > 0.05). Catalase and low salinity stress test show that CM0, SBM20.5, and CM20.5 were not significantly (<i>p</i> > 0.05) different, while CM33 was significantly lower than the rest of the diets. TBARs show that CM20.5 and CM33 diets were significantly different (<i>p</i> < 0.05), but CM20.5 was not significantly different from SBM20.5. Significantly higher hepatic <i>IGF-1</i> and <i>IGF-2</i> mRNA expression was found in fish-fed diet groups CM0, SBM20.5, and CM20.5 than fish fed CM33. The present study indicated that the addition of CM up 205 kg/kg to diet maintains growth, digestive enzymes, nutrient digestibility, immunity, stress resistance, and feed utilization efficiency of red sea bream.https://www.mdpi.com/2076-2615/11/11/3118plant protein mealred seabreamfish mealhealth statusgrowth-promoting genes |
| spellingShingle | Kumbukani Mzengereza Manabu Ishikawa Shunsuke Koshio Saichiro Yokoyama Zhang Yukun Ronick S. Shadrack Seok Seo Tomonari Kotani Serge Dossou Mohammed F. El Basuini Mahmoud A. O. Dawood Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>) Animals plant protein meal red seabream fish meal health status growth-promoting genes |
| title | Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>) |
| title_full | Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>) |
| title_fullStr | Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>) |
| title_full_unstemmed | Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>) |
| title_short | Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>) |
| title_sort | growth performance growth related genes digestibility digestive enzyme activity immune and stress responses of i de novo i camelina meal in diets of red seabream i pagrus major i |
| topic | plant protein meal red seabream fish meal health status growth-promoting genes |
| url | https://www.mdpi.com/2076-2615/11/11/3118 |
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