Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves
This study was conducted to investigate the impact of probiotic supplementation using <i>Lactobacillus plantarum</i> DSA 20174 and/or <i>Bacillus toyonensis</i> ATCC 55050 on growth performance, blood parameters, hematological measures, and fecal microbiota in pre-weaning Hol...
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MDPI AG
2023-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/21/3422 |
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| author | Mohamed S. Ayyat Hamdy A. El-Nagar Wael M. Wafa Khaled M. Abd El-Latif Samir Mahgoub Adham A. Al-Sagheer |
| author_facet | Mohamed S. Ayyat Hamdy A. El-Nagar Wael M. Wafa Khaled M. Abd El-Latif Samir Mahgoub Adham A. Al-Sagheer |
| author_sort | Mohamed S. Ayyat |
| collection | DOAJ |
| description | This study was conducted to investigate the impact of probiotic supplementation using <i>Lactobacillus plantarum</i> DSA 20174 and/or <i>Bacillus toyonensis</i> ATCC 55050 on growth performance, blood parameters, hematological measures, and fecal microbiota in pre-weaning Holstein calves. Thirty-two four-day-old male calves with a similar genetic background, weighing an average of 38.27 ± 0.12 kg, were randomly assigned to four groups. The groups consisted of a control group (CON) without supplementation, a group receiving <i>B. toyonensis</i> (BT) at 3 × 10<sup>9</sup> cfu/calf/day, a group receiving <i>L. plantarum</i> (LP) at 1 × 10<sup>10</sup> cfu/calf/day, and a group receiving a combination of LP and BT (LP + BT) at half the dosage for each. The study found that calves supplemented with LP and LP + BT experienced significant improvements in average daily gain and final body weight compared to the control group. The LP + BT group showed the most positive effects on TDMI, starter intake, and CP intake. RBC counts tended to be higher in the probiotic groups, with the LP + BT group having the highest values. The LP + BT group also had higher total protein, albumin, globulin, and hematocrit concentrations. All probiotic groups showed higher serum IgG concentrations. Probiotic supplementation led to increased total bacterial count and decreased levels of <i>E. coli</i>, salmonella, and clostridium. The LP + BT group had a significant decrease in coliform count, while both LP and LP + BT groups had increased <i>Lactobacillus</i> populations. In conclusion, LP + BT probiotic supplement showed the most beneficial effects on growth, feed efficiency, blood constituents, and modulation of fecal microbiota composition. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T11:34:27Z |
| publishDate | 2023-11-01 |
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| record_format | Article |
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| spelling | doaj.art-310d98f2c12d4b408068a6f71c4be7a22023-11-10T14:58:04ZengMDPI AGAnimals2076-26152023-11-011321342210.3390/ani13213422Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male CalvesMohamed S. Ayyat0Hamdy A. El-Nagar1Wael M. Wafa2Khaled M. Abd El-Latif3Samir Mahgoub4Adham A. Al-Sagheer5Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptAnimal Production Research Institute, Agricultural Research Center, Giza 12619, EgyptAnimal Production Research Institute, Agricultural Research Center, Giza 12619, EgyptSpecialized Hospital, Ain Shams University, Cairo 11588, EgyptDepartment of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptDepartment of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig 44511, EgyptThis study was conducted to investigate the impact of probiotic supplementation using <i>Lactobacillus plantarum</i> DSA 20174 and/or <i>Bacillus toyonensis</i> ATCC 55050 on growth performance, blood parameters, hematological measures, and fecal microbiota in pre-weaning Holstein calves. Thirty-two four-day-old male calves with a similar genetic background, weighing an average of 38.27 ± 0.12 kg, were randomly assigned to four groups. The groups consisted of a control group (CON) without supplementation, a group receiving <i>B. toyonensis</i> (BT) at 3 × 10<sup>9</sup> cfu/calf/day, a group receiving <i>L. plantarum</i> (LP) at 1 × 10<sup>10</sup> cfu/calf/day, and a group receiving a combination of LP and BT (LP + BT) at half the dosage for each. The study found that calves supplemented with LP and LP + BT experienced significant improvements in average daily gain and final body weight compared to the control group. The LP + BT group showed the most positive effects on TDMI, starter intake, and CP intake. RBC counts tended to be higher in the probiotic groups, with the LP + BT group having the highest values. The LP + BT group also had higher total protein, albumin, globulin, and hematocrit concentrations. All probiotic groups showed higher serum IgG concentrations. Probiotic supplementation led to increased total bacterial count and decreased levels of <i>E. coli</i>, salmonella, and clostridium. The LP + BT group had a significant decrease in coliform count, while both LP and LP + BT groups had increased <i>Lactobacillus</i> populations. In conclusion, LP + BT probiotic supplement showed the most beneficial effects on growth, feed efficiency, blood constituents, and modulation of fecal microbiota composition.https://www.mdpi.com/2076-2615/13/21/3422dairy calfprobiotic<i>Lactobacillus plantarum</i>Bacillus toyonensisgrowthhematology |
| spellingShingle | Mohamed S. Ayyat Hamdy A. El-Nagar Wael M. Wafa Khaled M. Abd El-Latif Samir Mahgoub Adham A. Al-Sagheer Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves Animals dairy calf probiotic <i>Lactobacillus plantarum</i> Bacillus toyonensis growth hematology |
| title | Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves |
| title_full | Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves |
| title_fullStr | Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves |
| title_full_unstemmed | Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves |
| title_short | Comparable Evaluation of Nutritional Benefits of <i>Lactobacillus plantarum</i> and <i>Bacillus toyonensis</i> Probiotic Supplementation on Growth, Feed Utilization, Health, and Fecal Microbiota in Pre-Weaning Male Calves |
| title_sort | comparable evaluation of nutritional benefits of i lactobacillus plantarum i and i bacillus toyonensis i probiotic supplementation on growth feed utilization health and fecal microbiota in pre weaning male calves |
| topic | dairy calf probiotic <i>Lactobacillus plantarum</i> Bacillus toyonensis growth hematology |
| url | https://www.mdpi.com/2076-2615/13/21/3422 |
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