A synthetic transcription platform for programmable gene expression in mammalian cells
Precise and scalable regulation of gene expression in mammalian cells is challenging. Here, the authors created a highly tunable CRISPR-based synthetic transcription system for programmable control of mammalian gene expression and cellular activity.
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33287-9 |
| _version_ | 1811336313178161152 |
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| author | William C. W. Chen Leonid Gaidukov Yong Lai Ming-Ru Wu Jicong Cao Michael J. Gutbrod Gigi C. G. Choi Rachel P. Utomo Ying-Chou Chen Liliana Wroblewska Manolis Kellis Lin Zhang Ron Weiss Timothy K. Lu |
| author_facet | William C. W. Chen Leonid Gaidukov Yong Lai Ming-Ru Wu Jicong Cao Michael J. Gutbrod Gigi C. G. Choi Rachel P. Utomo Ying-Chou Chen Liliana Wroblewska Manolis Kellis Lin Zhang Ron Weiss Timothy K. Lu |
| author_sort | William C. W. Chen |
| collection | DOAJ |
| description | Precise and scalable regulation of gene expression in mammalian cells is challenging. Here, the authors created a highly tunable CRISPR-based synthetic transcription system for programmable control of mammalian gene expression and cellular activity. |
| first_indexed | 2024-04-13T17:37:36Z |
| format | Article |
| id | doaj.art-310dc9b5a9a34df28dfb75c848485687 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T17:37:36Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-310dc9b5a9a34df28dfb75c8484856872022-12-22T02:37:17ZengNature PortfolioNature Communications2041-17232022-10-0113111610.1038/s41467-022-33287-9A synthetic transcription platform for programmable gene expression in mammalian cellsWilliam C. W. Chen0Leonid Gaidukov1Yong Lai2Ming-Ru Wu3Jicong Cao4Michael J. Gutbrod5Gigi C. G. Choi6Rachel P. Utomo7Ying-Chou Chen8Liliana Wroblewska9Manolis Kellis10Lin Zhang11Ron Weiss12Timothy K. Lu13Synthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologyComputer Science and Artificial Intelligence Laboratory, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologyPfizer Inc.Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of TechnologyPfizer Inc.Synthetic Biology Center, Massachusetts Institute of TechnologySynthetic Biology Center, Massachusetts Institute of TechnologyPrecise and scalable regulation of gene expression in mammalian cells is challenging. Here, the authors created a highly tunable CRISPR-based synthetic transcription system for programmable control of mammalian gene expression and cellular activity.https://doi.org/10.1038/s41467-022-33287-9 |
| spellingShingle | William C. W. Chen Leonid Gaidukov Yong Lai Ming-Ru Wu Jicong Cao Michael J. Gutbrod Gigi C. G. Choi Rachel P. Utomo Ying-Chou Chen Liliana Wroblewska Manolis Kellis Lin Zhang Ron Weiss Timothy K. Lu A synthetic transcription platform for programmable gene expression in mammalian cells Nature Communications |
| title | A synthetic transcription platform for programmable gene expression in mammalian cells |
| title_full | A synthetic transcription platform for programmable gene expression in mammalian cells |
| title_fullStr | A synthetic transcription platform for programmable gene expression in mammalian cells |
| title_full_unstemmed | A synthetic transcription platform for programmable gene expression in mammalian cells |
| title_short | A synthetic transcription platform for programmable gene expression in mammalian cells |
| title_sort | synthetic transcription platform for programmable gene expression in mammalian cells |
| url | https://doi.org/10.1038/s41467-022-33287-9 |
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