The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines
Sesquiterpenoids belong to a group of terpenoid compounds with interesting structures that are abundant in natural products especially in higher plants. Sesquiterpenoids have a wide variety of bioactivities with great potential cytotoxic activity. The species Dysoxylum excelsum belongs to the Meliac...
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| Format: | Article |
| Language: | English |
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Jenderal Soedirman University
2024-03-01
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| Series: | Molekul |
| Online Access: | http://jos.unsoed.ac.id/index.php/jm/article/view/9406 |
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| author | Unang Supratman Arsi Kautsari Al Arofatus Naini Sandra Amalia Riyadi Tri Mayanti Harizon Harizon Sofa Fajriah |
| author_facet | Unang Supratman Arsi Kautsari Al Arofatus Naini Sandra Amalia Riyadi Tri Mayanti Harizon Harizon Sofa Fajriah |
| author_sort | Unang Supratman |
| collection | DOAJ |
| description | Sesquiterpenoids belong to a group of terpenoid compounds with interesting structures that are abundant in natural products especially in higher plants. Sesquiterpenoids have a wide variety of bioactivities with great potential cytotoxic activity. The species Dysoxylum excelsum belongs to the Meliaceae family known as higher plant, but only a few sesquiterpenoids have been reported particularly for their cytotoxic activity. Therefore, this research aims to isolate and elucidate the sesquiterpenoids structure from D. excelsum stem bark and examines their cytotoxicity against HeLa cervical cancer cells. Through various column chromatography separations, four known sesquiterpenes namely β-caryophyllene oxide (1), caryophyllenol II (2), humulene dioxide A (3), and guai-6-en-10β-ol (4) were acquired from the n-hexane extract. Compounds 1-4 were isolated for the first time from D. excelsum species. The sesquiterpenoid structures were elucidated according to Nuclear Magnetic Resonance, Infrared, and HR-TOF-MS analysis. The cytotoxicity compounds 1-4 was determined against HeLa cervical cancer cells by examination with the PrestoBlue method and compound 3 exhibited the most potent cytotoxicity with an IC50 value of 160.74 µM.
Keywords: cytotoxic activity, Dysoxylum excelsum, HeLa, Meliaceae, sesquiterpenoid. |
| first_indexed | 2024-04-24T13:07:06Z |
| format | Article |
| id | doaj.art-311037f1f5734fccbce63d2d84ccf1a0 |
| institution | Directory Open Access Journal |
| issn | 1907-9761 2503-0310 |
| language | English |
| last_indexed | 2024-04-24T13:07:06Z |
| publishDate | 2024-03-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Molekul |
| spelling | doaj.art-311037f1f5734fccbce63d2d84ccf1a02024-04-05T06:44:58ZengJenderal Soedirman UniversityMolekul1907-97612503-03102024-03-0119110911610.20884/1.jm.2024.19.1.94069406The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell LinesUnang Supratman0Arsi Kautsari1Al Arofatus Naini2Sandra Amalia Riyadi3Tri Mayanti4Harizon Harizon5Sofa Fajriah6Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaFaculty of Teacher Training and Education, Universitas Jambi, Mendalo Indah, Jambi 36361, IndonesiaResearch Center for Chemistry, Indonesian Science Institute, Serpong 15311, South Tangerang, IndonesiaSesquiterpenoids belong to a group of terpenoid compounds with interesting structures that are abundant in natural products especially in higher plants. Sesquiterpenoids have a wide variety of bioactivities with great potential cytotoxic activity. The species Dysoxylum excelsum belongs to the Meliaceae family known as higher plant, but only a few sesquiterpenoids have been reported particularly for their cytotoxic activity. Therefore, this research aims to isolate and elucidate the sesquiterpenoids structure from D. excelsum stem bark and examines their cytotoxicity against HeLa cervical cancer cells. Through various column chromatography separations, four known sesquiterpenes namely β-caryophyllene oxide (1), caryophyllenol II (2), humulene dioxide A (3), and guai-6-en-10β-ol (4) were acquired from the n-hexane extract. Compounds 1-4 were isolated for the first time from D. excelsum species. The sesquiterpenoid structures were elucidated according to Nuclear Magnetic Resonance, Infrared, and HR-TOF-MS analysis. The cytotoxicity compounds 1-4 was determined against HeLa cervical cancer cells by examination with the PrestoBlue method and compound 3 exhibited the most potent cytotoxicity with an IC50 value of 160.74 µM. Keywords: cytotoxic activity, Dysoxylum excelsum, HeLa, Meliaceae, sesquiterpenoid.http://jos.unsoed.ac.id/index.php/jm/article/view/9406 |
| spellingShingle | Unang Supratman Arsi Kautsari Al Arofatus Naini Sandra Amalia Riyadi Tri Mayanti Harizon Harizon Sofa Fajriah The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines Molekul |
| title | The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines |
| title_full | The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines |
| title_fullStr | The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines |
| title_full_unstemmed | The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines |
| title_short | The Sesquiterpenoids from the Stem Bark of Dysoxylum excelsum and Their Cytotoxic Activities against HeLa Cancer Cell Lines |
| title_sort | sesquiterpenoids from the stem bark of dysoxylum excelsum and their cytotoxic activities against hela cancer cell lines |
| url | http://jos.unsoed.ac.id/index.php/jm/article/view/9406 |
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