Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice
Objective(s): Kaempferide (Ka), a major natural active component of Tagetes erecta L, has numerous pharmacological effects such as anti-obesity, anticancer, and anti-hypertension. However, there is no clear evidence that Ka is directly related to inflammation and oxidative stress in obese mice. We a...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-04-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_17744_d8277b2de3faa8d72fbce6f30bf69d66.pdf |
| _version_ | 1818886190929018880 |
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| author | Heng Tang Qingfu Zeng Nina Ren Yunjie Wei Quan He Ming Chen Peng Pu |
| author_facet | Heng Tang Qingfu Zeng Nina Ren Yunjie Wei Quan He Ming Chen Peng Pu |
| author_sort | Heng Tang |
| collection | DOAJ |
| description | Objective(s): Kaempferide (Ka), a major natural active component of Tagetes erecta L, has numerous pharmacological effects such as anti-obesity, anticancer, and anti-hypertension. However, there is no clear evidence that Ka is directly related to inflammation and oxidative stress in obese mice. We aimed to explore the effects of Ka on inflammation and oxidative stress and its mechanism.Materials and Methods: The obese mice were induced by a high-fat diet (HFD). The anti-obesity effect was tested by liver and body weight, liver and adiposity index, and white adipose tissue. Blood sample analysis was used to detect the hypolipidemic and hypoglycemic effects. The anti-oxidation effect was assessed using GSH, SOD, MDA, CAT, T-AOC, and other indicators. The anti-inflammatory effect was assessed using TNF-α, MCP-1, and Adiponectin. Western blot and Real-Time PCR were used to evaluate the related signaling pathways.Results: Obesity, glycolipid metabolism disorder, inflammation, and oxidative stress developed in HFD mice. These changes can be effectively alleviated by Ka treatment for 16 weeks. Further studies have suggested that these beneficial effects of Ka may be associated with inhibition of the TLR4/IκBα/NF-κB signaling pathways. Conclusion: Ka possesses important anti-obesity, hypoglycemic, and hypolipidemic effects. The mechanism may be causally associated with the TLR4/IκBα/NF-κB signaling pathway, which improves inflammation and oxidative stress. |
| first_indexed | 2024-12-19T16:17:24Z |
| format | Article |
| id | doaj.art-31143508b203422fbed828ca32123af7 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-19T16:17:24Z |
| publishDate | 2021-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-31143508b203422fbed828ca32123af72022-12-21T20:14:35ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-04-0124449349810.22038/ijbms.2021.52690.1189217744Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese miceHeng Tang0Qingfu Zeng1Nina Ren2Yunjie Wei3Quan He4Ming Chen5Peng Pu6Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR ChinaDepartment of vascular surgery, The Second Affiliated Hospital of Nanchang UniversityGuangdong Online Hospital Clinic, Guangdong Second Provincial People’s Hospital, Guangzhou 510317, PR ChinaTaihe Hospital Shiyan, Hubei, PR ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR ChinaDepartment of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR ChinaObjective(s): Kaempferide (Ka), a major natural active component of Tagetes erecta L, has numerous pharmacological effects such as anti-obesity, anticancer, and anti-hypertension. However, there is no clear evidence that Ka is directly related to inflammation and oxidative stress in obese mice. We aimed to explore the effects of Ka on inflammation and oxidative stress and its mechanism.Materials and Methods: The obese mice were induced by a high-fat diet (HFD). The anti-obesity effect was tested by liver and body weight, liver and adiposity index, and white adipose tissue. Blood sample analysis was used to detect the hypolipidemic and hypoglycemic effects. The anti-oxidation effect was assessed using GSH, SOD, MDA, CAT, T-AOC, and other indicators. The anti-inflammatory effect was assessed using TNF-α, MCP-1, and Adiponectin. Western blot and Real-Time PCR were used to evaluate the related signaling pathways.Results: Obesity, glycolipid metabolism disorder, inflammation, and oxidative stress developed in HFD mice. These changes can be effectively alleviated by Ka treatment for 16 weeks. Further studies have suggested that these beneficial effects of Ka may be associated with inhibition of the TLR4/IκBα/NF-κB signaling pathways. Conclusion: Ka possesses important anti-obesity, hypoglycemic, and hypolipidemic effects. The mechanism may be causally associated with the TLR4/IκBα/NF-κB signaling pathway, which improves inflammation and oxidative stress.https://ijbms.mums.ac.ir/article_17744_d8277b2de3faa8d72fbce6f30bf69d66.pdfantiinflammatory antioxidation kaempferide obesity tlr4 |
| spellingShingle | Heng Tang Qingfu Zeng Nina Ren Yunjie Wei Quan He Ming Chen Peng Pu Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice Iranian Journal of Basic Medical Sciences anti inflammatory anti oxidation kaempferide obesity tlr4 |
| title | Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice |
| title_full | Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice |
| title_fullStr | Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice |
| title_full_unstemmed | Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice |
| title_short | Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice |
| title_sort | kaempferide improves oxidative stress and inflammation by inhibiting the tlr4 iκbα nf κb pathway in obese mice |
| topic | anti inflammatory anti oxidation kaempferide obesity tlr4 |
| url | https://ijbms.mums.ac.ir/article_17744_d8277b2de3faa8d72fbce6f30bf69d66.pdf |
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