New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress
Introduction: Antifreeze peptides regulate the physiological functions of frozen cells and even their apoptosis; however, the mechanisms by which antifreeze peptides regulate these processes remain unclear, although the interactions between cell membranes and ice are well known to be important in th...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Advanced Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209012322200114X |
| _version_ | 1797894382471348224 |
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| author | Xu Chen Jinhong Wu Fujia Yang Mi Zhou Ruibin Wang Jianlian Huang Yuzhi Rong Jianhua Liu Shaoyun Wang |
| author_facet | Xu Chen Jinhong Wu Fujia Yang Mi Zhou Ruibin Wang Jianlian Huang Yuzhi Rong Jianhua Liu Shaoyun Wang |
| author_sort | Xu Chen |
| collection | DOAJ |
| description | Introduction: Antifreeze peptides regulate the physiological functions of frozen cells and even their apoptosis; however, the mechanisms by which antifreeze peptides regulate these processes remain unclear, although the interactions between cell membranes and ice are well known to be important in this process. Objectives: Our study aims to investigate how antifreeze peptides regulate cell physiological functions during the freezing process. Methods: We investigated the cryoprotective effect of rsfAFP on the physiological functions of S. thermophilus under freezing stress by measuring cellular metabolism activity, intracellular enzyme activity, cell membrane characterization, and cell apoptosis. The mechanism by which rsfAFP impacts S. thermophilus physiological functions under freezing stress was investigated using multispectral techniques and cryo-TEM. Results: We show that a recombinant antifreeze peptide (rsfAFP) interacts with the extracellular capsular polysaccharides and peptidoglycan of Streptococcus thermophilus and ice to cover the outer layer of the membrane, forming a dense protective layer that regulates the molecular structure of extracellular ice crystals, which results in reduced extracellular membrane damage, depressed apoptosis and increased intracellular metabolic activity. This interaction mechanism was indicated by the fact that S. thermophilus better maintained its permeability barrier, membrane fluidity, membrane structural integrity, and cytoplasmic membrane potential during freezing stress with rsfAFP treatment. Conclusion: These results provide new insights into the mechanism by which rsfAFP regulates frozen cell physiological functions and apoptosis under freezing stress. |
| first_indexed | 2024-04-10T07:08:08Z |
| format | Article |
| id | doaj.art-31149bea2b6e408ebc1ef8e10917a8bc |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-04-10T07:08:08Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-31149bea2b6e408ebc1ef8e10917a8bc2023-02-27T04:07:03ZengElsevierJournal of Advanced Research2090-12322023-03-0145127140New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stressXu Chen0Jinhong Wu1Fujia Yang2Mi Zhou3Ruibin Wang4Jianlian Huang5Yuzhi Rong6Jianhua Liu7Shaoyun Wang8School of Mechanical Science & Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, ChinaDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, ChinaDepartment of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaInstrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, ChinaKey Laboratory of Refrigeration and Conditioning Aquatic Products Processing of Ministry of Agriculture and Rural Affairs, Xiamen 361022, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaInternational Faculty of Applied Technology, Yibin University, Yibin, Sichuan 644000, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China; Corresponding author.Introduction: Antifreeze peptides regulate the physiological functions of frozen cells and even their apoptosis; however, the mechanisms by which antifreeze peptides regulate these processes remain unclear, although the interactions between cell membranes and ice are well known to be important in this process. Objectives: Our study aims to investigate how antifreeze peptides regulate cell physiological functions during the freezing process. Methods: We investigated the cryoprotective effect of rsfAFP on the physiological functions of S. thermophilus under freezing stress by measuring cellular metabolism activity, intracellular enzyme activity, cell membrane characterization, and cell apoptosis. The mechanism by which rsfAFP impacts S. thermophilus physiological functions under freezing stress was investigated using multispectral techniques and cryo-TEM. Results: We show that a recombinant antifreeze peptide (rsfAFP) interacts with the extracellular capsular polysaccharides and peptidoglycan of Streptococcus thermophilus and ice to cover the outer layer of the membrane, forming a dense protective layer that regulates the molecular structure of extracellular ice crystals, which results in reduced extracellular membrane damage, depressed apoptosis and increased intracellular metabolic activity. This interaction mechanism was indicated by the fact that S. thermophilus better maintained its permeability barrier, membrane fluidity, membrane structural integrity, and cytoplasmic membrane potential during freezing stress with rsfAFP treatment. Conclusion: These results provide new insights into the mechanism by which rsfAFP regulates frozen cell physiological functions and apoptosis under freezing stress.http://www.sciencedirect.com/science/article/pii/S209012322200114XAntifreeze peptideStreptococcus thermophilusPhysiological functionMembrane structureCryoprotective mechanism |
| spellingShingle | Xu Chen Jinhong Wu Fujia Yang Mi Zhou Ruibin Wang Jianlian Huang Yuzhi Rong Jianhua Liu Shaoyun Wang New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress Journal of Advanced Research Antifreeze peptide Streptococcus thermophilus Physiological function Membrane structure Cryoprotective mechanism |
| title | New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress |
| title_full | New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress |
| title_fullStr | New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress |
| title_full_unstemmed | New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress |
| title_short | New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress |
| title_sort | new insight into the mechanism by which antifreeze peptides regulate the physiological function of streptococcus thermophilus subjected to freezing stress |
| topic | Antifreeze peptide Streptococcus thermophilus Physiological function Membrane structure Cryoprotective mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S209012322200114X |
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