Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets
ABSTRACT: The objective of this study is to investigate the beneficial effects and underlying mechanism of dietary β-mannanase supplementation on the productive performance of laying hens fed with metabolic energy (ME)-reduced diets. A total of 448 Hy-Line gray laying hens were randomly assigned to...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124001007 |
| _version_ | 1797256469555445760 |
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| author | Huawei Liu Weiyong Liu Mingming Ai Xiaojing Hao Qian Zhang Jingle Ren Kai Zhang |
| author_facet | Huawei Liu Weiyong Liu Mingming Ai Xiaojing Hao Qian Zhang Jingle Ren Kai Zhang |
| author_sort | Huawei Liu |
| collection | DOAJ |
| description | ABSTRACT: The objective of this study is to investigate the beneficial effects and underlying mechanism of dietary β-mannanase supplementation on the productive performance of laying hens fed with metabolic energy (ME)-reduced diets. A total of 448 Hy-Line gray laying hens were randomly assigned to seven groups. Each group had 8 replicates with 8 hens. The groups included a control diet (CON) with a ME of 2750 kcal/Kg, diets reduced by 100 kcal/Kg or 200 kcal/Kg ME (ME_100 or ME_200), and diets with 0.15 g/Kg or 0.2 g/Kg β-mannanase (ME_100+β-M_0.15, ME_100+β-M_0.2, ME_200+β-M_0.15, and ME_200+β-M_0.2). The productive performance, egg quality, intestinal morphology, inflammatory response, mRNA expression related to the Nuclear factor kappa B (NF-κB) and AMPK pathway, and cecum microbiome were evaluated in this study. ME-reduced diets negatively impacted the productive performance of laying hens. However, supplementation with β-mannanase improved FCR, decreased ADFI, and restored average egg weight to the level of the CON group. ME-reduced diets increased the levels of interleukin-1β (IL-1β) and IL-6 while decreasing the levels of IL-4 and IL-10 in the jejunum of laying hens. However, dietary β-mannanase supplementation improved jejunum morphology, reduced pro-inflammatory cytokine concentrations, and increased levels of anti-inflammatory factors in laying hens fed with ME-reduced diets. The mRNA levels of IL-6, IFN-γ, TLR4, MyD88, and NF-κB in the jejunum of ME-reduced diets were significantly higher than that in CON, dietary β-mannanase supplementation decreased these genes expression in laying hens fed with ME-reduced diets. Moreover, dietary β-mannanase supplementation also decreased the mRNA levels of AMPKα and AMPKγ, and increased the abundance of mTOR in the jejunum of laying hens fed with ME-reduced diets. Cecum microbiota analysis revealed that dietary β-mannanase increased the abundance of various beneficial bacteria (e.g., g_Pseudoflavonifractor, g_Butyricicoccus, and f_Lactobacillaceae) in laying hens fed with ME-reduced diets. In conclusion, dietary β-mannanase supplementation could improve the productive performance of laying hens fed with a ME-reduced diet by improving intestinal morphology, alleviating intestinal inflammation, changing energy metabolism-related signaling pathways, and increasing cecum-beneficial microbiota. |
| first_indexed | 2024-03-08T00:01:40Z |
| format | Article |
| id | doaj.art-3114f91c7ccd40cc9a1798c66e442dec |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T22:22:14Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj.art-3114f91c7ccd40cc9a1798c66e442dec2024-03-20T06:07:56ZengElsevierPoultry Science0032-57912024-04-011034103521Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy dietsHuawei Liu0Weiyong Liu1Mingming Ai2Xiaojing Hao3Qian Zhang4Jingle Ren5Kai Zhang6College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaQingdao Animal Husbandry Workstation (Qingdao Research Institute of Husbandry and Veterinary), Qingdao 266100, ChinaQingdao Animal Husbandry Workstation (Qingdao Research Institute of Husbandry and Veterinary), Qingdao 266100, ChinaQingdao Animal Husbandry Workstation (Qingdao Research Institute of Husbandry and Veterinary), Qingdao 266100, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China; Corresponding author:ABSTRACT: The objective of this study is to investigate the beneficial effects and underlying mechanism of dietary β-mannanase supplementation on the productive performance of laying hens fed with metabolic energy (ME)-reduced diets. A total of 448 Hy-Line gray laying hens were randomly assigned to seven groups. Each group had 8 replicates with 8 hens. The groups included a control diet (CON) with a ME of 2750 kcal/Kg, diets reduced by 100 kcal/Kg or 200 kcal/Kg ME (ME_100 or ME_200), and diets with 0.15 g/Kg or 0.2 g/Kg β-mannanase (ME_100+β-M_0.15, ME_100+β-M_0.2, ME_200+β-M_0.15, and ME_200+β-M_0.2). The productive performance, egg quality, intestinal morphology, inflammatory response, mRNA expression related to the Nuclear factor kappa B (NF-κB) and AMPK pathway, and cecum microbiome were evaluated in this study. ME-reduced diets negatively impacted the productive performance of laying hens. However, supplementation with β-mannanase improved FCR, decreased ADFI, and restored average egg weight to the level of the CON group. ME-reduced diets increased the levels of interleukin-1β (IL-1β) and IL-6 while decreasing the levels of IL-4 and IL-10 in the jejunum of laying hens. However, dietary β-mannanase supplementation improved jejunum morphology, reduced pro-inflammatory cytokine concentrations, and increased levels of anti-inflammatory factors in laying hens fed with ME-reduced diets. The mRNA levels of IL-6, IFN-γ, TLR4, MyD88, and NF-κB in the jejunum of ME-reduced diets were significantly higher than that in CON, dietary β-mannanase supplementation decreased these genes expression in laying hens fed with ME-reduced diets. Moreover, dietary β-mannanase supplementation also decreased the mRNA levels of AMPKα and AMPKγ, and increased the abundance of mTOR in the jejunum of laying hens fed with ME-reduced diets. Cecum microbiota analysis revealed that dietary β-mannanase increased the abundance of various beneficial bacteria (e.g., g_Pseudoflavonifractor, g_Butyricicoccus, and f_Lactobacillaceae) in laying hens fed with ME-reduced diets. In conclusion, dietary β-mannanase supplementation could improve the productive performance of laying hens fed with a ME-reduced diet by improving intestinal morphology, alleviating intestinal inflammation, changing energy metabolism-related signaling pathways, and increasing cecum-beneficial microbiota.http://www.sciencedirect.com/science/article/pii/S0032579124001007laying hensβ-Mannanaselow-energy dietinflammatory responsececum microbiota |
| spellingShingle | Huawei Liu Weiyong Liu Mingming Ai Xiaojing Hao Qian Zhang Jingle Ren Kai Zhang Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets Poultry Science laying hens β-Mannanase low-energy diet inflammatory response cecum microbiota |
| title | Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets |
| title_full | Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets |
| title_fullStr | Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets |
| title_full_unstemmed | Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets |
| title_short | Effects of β-mannanase supplementation on productive performance, inflammation, energy metabolism, and cecum microbiota composition of laying hens fed with reduced-energy diets |
| title_sort | effects of β mannanase supplementation on productive performance inflammation energy metabolism and cecum microbiota composition of laying hens fed with reduced energy diets |
| topic | laying hens β-Mannanase low-energy diet inflammatory response cecum microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124001007 |
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