F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans.
Fungal growth and development are coordinated with specific secondary metabolism. This coordination requires 8 of 74 F-box proteins of the filamentous fungus Aspergillus nidulans. F-box proteins recognize primed substrates for ubiquitination by Skp1-Cul1-Fbx (SCF) E3 ubiquitin RING ligases and degra...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-12-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010502 |
| _version_ | 1797973905414029312 |
|---|---|
| author | Özlem Sarikaya Bayram Özgür Bayram Betim Karahoda Cindy Meister Anna M Köhler Sabine Thieme Nadia Elramli Dean Frawley Jamie McGowan David A Fitzpatrick Kerstin Schmitt Leandro Jose de Assis Oliver Valerius Gustavo H Goldman Gerhard H Braus |
| author_facet | Özlem Sarikaya Bayram Özgür Bayram Betim Karahoda Cindy Meister Anna M Köhler Sabine Thieme Nadia Elramli Dean Frawley Jamie McGowan David A Fitzpatrick Kerstin Schmitt Leandro Jose de Assis Oliver Valerius Gustavo H Goldman Gerhard H Braus |
| author_sort | Özlem Sarikaya Bayram |
| collection | DOAJ |
| description | Fungal growth and development are coordinated with specific secondary metabolism. This coordination requires 8 of 74 F-box proteins of the filamentous fungus Aspergillus nidulans. F-box proteins recognize primed substrates for ubiquitination by Skp1-Cul1-Fbx (SCF) E3 ubiquitin RING ligases and degradation by the 26S proteasome. 24 F-box proteins are found in the nuclear fraction as part of SCFs during vegetative growth. 43 F-box proteins interact with SCF proteins during growth, development or stress. 45 F-box proteins are associated with more than 700 proteins that have mainly regulatory roles. This corroborates that accurate surveillance of protein stability is prerequisite for organizing multicellular fungal development. Fbx23 combines subcellular location and protein stability control, illustrating the complexity of F-box mediated regulation during fungal development. Fbx23 interacts with epigenetic methyltransferase VipC which interacts with fungal NF-κB-like velvet domain regulator VeA that coordinates fungal development with secondary metabolism. Fbx23 prevents nuclear accumulation of methyltransferase VipC during early development. These results suggest that in addition to their role in protein degradation, F-box proteins also control subcellular accumulations of key regulatory proteins for fungal development. |
| first_indexed | 2024-04-11T04:11:37Z |
| format | Article |
| id | doaj.art-3115c33e38be48dc882ac0bdb967cd50 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-11T04:11:37Z |
| publishDate | 2022-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-3115c33e38be48dc882ac0bdb967cd502023-01-01T05:31:58ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-12-011812e101050210.1371/journal.pgen.1010502F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans.Özlem Sarikaya BayramÖzgür BayramBetim KarahodaCindy MeisterAnna M KöhlerSabine ThiemeNadia ElramliDean FrawleyJamie McGowanDavid A FitzpatrickKerstin SchmittLeandro Jose de AssisOliver ValeriusGustavo H GoldmanGerhard H BrausFungal growth and development are coordinated with specific secondary metabolism. This coordination requires 8 of 74 F-box proteins of the filamentous fungus Aspergillus nidulans. F-box proteins recognize primed substrates for ubiquitination by Skp1-Cul1-Fbx (SCF) E3 ubiquitin RING ligases and degradation by the 26S proteasome. 24 F-box proteins are found in the nuclear fraction as part of SCFs during vegetative growth. 43 F-box proteins interact with SCF proteins during growth, development or stress. 45 F-box proteins are associated with more than 700 proteins that have mainly regulatory roles. This corroborates that accurate surveillance of protein stability is prerequisite for organizing multicellular fungal development. Fbx23 combines subcellular location and protein stability control, illustrating the complexity of F-box mediated regulation during fungal development. Fbx23 interacts with epigenetic methyltransferase VipC which interacts with fungal NF-κB-like velvet domain regulator VeA that coordinates fungal development with secondary metabolism. Fbx23 prevents nuclear accumulation of methyltransferase VipC during early development. These results suggest that in addition to their role in protein degradation, F-box proteins also control subcellular accumulations of key regulatory proteins for fungal development.https://doi.org/10.1371/journal.pgen.1010502 |
| spellingShingle | Özlem Sarikaya Bayram Özgür Bayram Betim Karahoda Cindy Meister Anna M Köhler Sabine Thieme Nadia Elramli Dean Frawley Jamie McGowan David A Fitzpatrick Kerstin Schmitt Leandro Jose de Assis Oliver Valerius Gustavo H Goldman Gerhard H Braus F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. PLoS Genetics |
| title | F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. |
| title_full | F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. |
| title_fullStr | F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. |
| title_full_unstemmed | F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. |
| title_short | F-box receptor mediated control of substrate stability and subcellular location organizes cellular development of Aspergillus nidulans. |
| title_sort | f box receptor mediated control of substrate stability and subcellular location organizes cellular development of aspergillus nidulans |
| url | https://doi.org/10.1371/journal.pgen.1010502 |
| work_keys_str_mv | AT ozlemsarikayabayram fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT ozgurbayram fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT betimkarahoda fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT cindymeister fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT annamkohler fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT sabinethieme fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT nadiaelramli fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT deanfrawley fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT jamiemcgowan fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT davidafitzpatrick fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT kerstinschmitt fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT leandrojosedeassis fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT olivervalerius fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT gustavohgoldman fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans AT gerhardhbraus fboxreceptormediatedcontrolofsubstratestabilityandsubcellularlocationorganizescellulardevelopmentofaspergillusnidulans |