Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering
In this work, the polycaprolactone (PCL) scaffold with specific surface morphology was prepared by cryogenic printing technique. Biocompatible hydroxyapatite (HA) was added to the PCL matrix to enhance the surface properties and bioactivity of the scaffolds. The effects of HA on the mechanical stren...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127521002641 |
| _version_ | 1818595552442449920 |
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| author | Yihan Li Zehao Yu Fanrong Ai Chunxuan Wu Kui Zhou Chuanliang Cao Wenchao Li |
| author_facet | Yihan Li Zehao Yu Fanrong Ai Chunxuan Wu Kui Zhou Chuanliang Cao Wenchao Li |
| author_sort | Yihan Li |
| collection | DOAJ |
| description | In this work, the polycaprolactone (PCL) scaffold with specific surface morphology was prepared by cryogenic printing technique. Biocompatible hydroxyapatite (HA) was added to the PCL matrix to enhance the surface properties and bioactivity of the scaffolds. The effects of HA on the mechanical strength and surface morphology were studied systematically. It was found that the extra surface morphology of filament enhanced the exposure of the HA particles, which induced a positive synergistic effect on surface properties. The wettability and bio-mineralization were significantly improved, and the protein adsorption capacity was 4.5 and 3.9 times than the ones of sample with 20%HA and smooth surface, respectively. Rat bone mesenchymal stem cells (rBMSCs) were used as the cell model to evaluate the cellular response to the specific surface of the scaffolds. The results showed that the scaffolds with 20–40% HA and extra surface had better proliferation ratio. The ALP assay also demonstrated that the synergistic effect was beneficial to cell osteogenic differentiation, and the ALP level was significantly higher than that of pure PCL and smooth PCL/HA composite scaffold samples at the 14th day. Therefore, the PCL/HA composite scaffold with extra surface morphology had better potential for bone tissue engineering. |
| first_indexed | 2024-12-16T11:17:50Z |
| format | Article |
| id | doaj.art-311d10b6a05548488f272f5e5e846882 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-16T11:17:50Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-311d10b6a05548488f272f5e5e8468822022-12-21T22:33:33ZengElsevierMaterials & Design0264-12752021-07-01205109712Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineeringYihan Li0Zehao Yu1Fanrong Ai2Chunxuan Wu3Kui Zhou4Chuanliang Cao5Wenchao Li6The First Clinical Medical School, Nanchang University, Nanchang, Jiangxi 330006, ChinaThe First Clinical Medical School, Nanchang University, Nanchang, Jiangxi 330006, ChinaSchool of Mechanic & Electronic Engineering, Nanchang University, Nanchang, Jiangxi 330031, ChinaThe Second Clinical Medical School, Nanchang University, Nanchang, Jiangxi 330006, ChinaSchool of Mechanic & Electronic Engineering, Nanchang University, Nanchang, Jiangxi 330031, ChinaSchool of Mechanic & Electronic Engineering, Nanchang University, Nanchang, Jiangxi 330031, ChinaSchool of Mechanic & Electronic Engineering, Nanchang University, Nanchang, Jiangxi 330031, China; Corresponding author.In this work, the polycaprolactone (PCL) scaffold with specific surface morphology was prepared by cryogenic printing technique. Biocompatible hydroxyapatite (HA) was added to the PCL matrix to enhance the surface properties and bioactivity of the scaffolds. The effects of HA on the mechanical strength and surface morphology were studied systematically. It was found that the extra surface morphology of filament enhanced the exposure of the HA particles, which induced a positive synergistic effect on surface properties. The wettability and bio-mineralization were significantly improved, and the protein adsorption capacity was 4.5 and 3.9 times than the ones of sample with 20%HA and smooth surface, respectively. Rat bone mesenchymal stem cells (rBMSCs) were used as the cell model to evaluate the cellular response to the specific surface of the scaffolds. The results showed that the scaffolds with 20–40% HA and extra surface had better proliferation ratio. The ALP assay also demonstrated that the synergistic effect was beneficial to cell osteogenic differentiation, and the ALP level was significantly higher than that of pure PCL and smooth PCL/HA composite scaffold samples at the 14th day. Therefore, the PCL/HA composite scaffold with extra surface morphology had better potential for bone tissue engineering.http://www.sciencedirect.com/science/article/pii/S0264127521002641Cryogenic printingPolycaprolactoneHydroxyapatiteSpecific surface morphologyBone tissue engineering |
| spellingShingle | Yihan Li Zehao Yu Fanrong Ai Chunxuan Wu Kui Zhou Chuanliang Cao Wenchao Li Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering Materials & Design Cryogenic printing Polycaprolactone Hydroxyapatite Specific surface morphology Bone tissue engineering |
| title | Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering |
| title_full | Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering |
| title_fullStr | Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering |
| title_full_unstemmed | Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering |
| title_short | Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering |
| title_sort | characterization and evaluation of polycaprolactone hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering |
| topic | Cryogenic printing Polycaprolactone Hydroxyapatite Specific surface morphology Bone tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S0264127521002641 |
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