Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice.
Entamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. There is currently no vaccine against this parasite. We have previously shown that intracecal inoculation of E. histolytica trophozoites leads to chronic and non-healing cecitis in...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3843725?pdf=render |
| _version_ | 1818840964369743872 |
|---|---|
| author | Chikako Shimokawa Richard Culleton Takashi Imai Kazutomo Suzue Makoto Hirai Tomoyo Taniguchi Seiki Kobayashi Hajime Hisaeda Shinjiro Hamano |
| author_facet | Chikako Shimokawa Richard Culleton Takashi Imai Kazutomo Suzue Makoto Hirai Tomoyo Taniguchi Seiki Kobayashi Hajime Hisaeda Shinjiro Hamano |
| author_sort | Chikako Shimokawa |
| collection | DOAJ |
| description | Entamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. There is currently no vaccine against this parasite. We have previously shown that intracecal inoculation of E. histolytica trophozoites leads to chronic and non-healing cecitis in mice. Entamoeba moshkovskii, a closely related amoeba, also causes diarrhea and other intestinal disorders in this model. Here, we investigated the effect of infection followed by drug-cure of these species on the induction of immunity against homologous or heterologous species challenge. Mice were infected with E. histolytica or E. moshkovskii and treated with metronidazole 14 days later. Re-challenge with E. histolytica or E. moshkovskii was conducted seven or 28 days following confirmation of the clearance of amoebae, and the degree of protection compared to non-exposed control mice was evaluated. We show that primary infection with these amoebae induces a species-specific immune response which protects against challenge with the homologous, but not a heterologous species. These findings pave the way, therefore, for the identification of novel amoebae antigens that may become the targets of vaccines and provide a useful platform to investigate host protective immunity to Entamoeba infections. |
| first_indexed | 2024-12-19T04:18:33Z |
| format | Article |
| id | doaj.art-311d7872727741e0a8a7750094cce9c9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T04:18:33Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-311d7872727741e0a8a7750094cce9c92022-12-21T20:36:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e8202510.1371/journal.pone.0082025Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice.Chikako ShimokawaRichard CulletonTakashi ImaiKazutomo SuzueMakoto HiraiTomoyo TaniguchiSeiki KobayashiHajime HisaedaShinjiro HamanoEntamoeba histolytica, the parasitic amoeba responsible for amoebiasis, causes approximately 100,000 deaths every year. There is currently no vaccine against this parasite. We have previously shown that intracecal inoculation of E. histolytica trophozoites leads to chronic and non-healing cecitis in mice. Entamoeba moshkovskii, a closely related amoeba, also causes diarrhea and other intestinal disorders in this model. Here, we investigated the effect of infection followed by drug-cure of these species on the induction of immunity against homologous or heterologous species challenge. Mice were infected with E. histolytica or E. moshkovskii and treated with metronidazole 14 days later. Re-challenge with E. histolytica or E. moshkovskii was conducted seven or 28 days following confirmation of the clearance of amoebae, and the degree of protection compared to non-exposed control mice was evaluated. We show that primary infection with these amoebae induces a species-specific immune response which protects against challenge with the homologous, but not a heterologous species. These findings pave the way, therefore, for the identification of novel amoebae antigens that may become the targets of vaccines and provide a useful platform to investigate host protective immunity to Entamoeba infections.http://europepmc.org/articles/PMC3843725?pdf=render |
| spellingShingle | Chikako Shimokawa Richard Culleton Takashi Imai Kazutomo Suzue Makoto Hirai Tomoyo Taniguchi Seiki Kobayashi Hajime Hisaeda Shinjiro Hamano Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. PLoS ONE |
| title | Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. |
| title_full | Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. |
| title_fullStr | Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. |
| title_full_unstemmed | Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. |
| title_short | Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice. |
| title_sort | species specific immunity induced by infection with entamoeba histolytica and entamoeba moshkovskii in mice |
| url | http://europepmc.org/articles/PMC3843725?pdf=render |
| work_keys_str_mv | AT chikakoshimokawa speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT richardculleton speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT takashiimai speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT kazutomosuzue speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT makotohirai speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT tomoyotaniguchi speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT seikikobayashi speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT hajimehisaeda speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice AT shinjirohamano speciesspecificimmunityinducedbyinfectionwithentamoebahistolyticaandentamoebamoshkovskiiinmice |