Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients
<i>Helicobacter pylori</i> infection possessing East-Asian-type CagA is associated with carcinogenesis. Mongolia has the highest mortality rate from gastric cancer. Therefore, we evaluated the CagA status in the Mongolian population. High risk and gastric cancer patients were determined...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/11/5/725 |
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| author | Tegshee Tserentogtokh Boldbaatar Gantuya Phawinee Subsomwong Khasag Oyuntsetseg Dashdorj Bolor Yansan Erdene-Ochir Dashdorj Azzaya Duger Davaadorj Tomohisa Uchida Takeshi Matsuhisa Yoshio Yamaoka |
| author_facet | Tegshee Tserentogtokh Boldbaatar Gantuya Phawinee Subsomwong Khasag Oyuntsetseg Dashdorj Bolor Yansan Erdene-Ochir Dashdorj Azzaya Duger Davaadorj Tomohisa Uchida Takeshi Matsuhisa Yoshio Yamaoka |
| author_sort | Tegshee Tserentogtokh |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> infection possessing East-Asian-type CagA is associated with carcinogenesis. Mongolia has the highest mortality rate from gastric cancer. Therefore, we evaluated the CagA status in the Mongolian population. High risk and gastric cancer patients were determined using endoscopy and histological examination. <i>H. pylori</i> strains were isolated from different locations in Mongolia. The CagA subtypes (East-Asian-type or Western-type, based on sequencing of Glu-Pro-Ile-Tyr-Ala (EPIYA) segments) and <i>vacA</i> genotypes (s and m regions) were determined using PCR-based sequencing and PCR, respectively. In total, 368 patients were examined (341 gastritis, 10 peptic ulcer, and 17 gastric cancer). Sixty-two (16.8%) strains were <i>cagA</i>-negative and 306 (83.1%) were <i>cagA</i>-positive (293 Western-type, 12 East-Asian-type, and one hybrid type). All <i>cagA</i>-negative strains were isolated from gastritis patients. In the gastritis group, 78.6% (268/341) had Western-type CagA, 2.9% (10/341) had East-Asian-type, and 18.2% (61/341) were <i>cagA</i>-negative. However, all <i>H. pylori</i> from gastric cancer patients possessed Western-type CagA. Histological analyses showed that East-Asian-type CagA was the most virulent strains, followed by Western-type and <i>cagA</i>-negative strains. This finding agreed with the current consensus. <i>CagA</i>-positive strains were the most virulent type. However, the fact that different CagA types can explain the high incidence of gastric cancer might be inapplicable in Mongolia. |
| first_indexed | 2024-03-12T07:13:17Z |
| format | Article |
| id | doaj.art-311f50d89786406e90cffb0e6b078392 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T07:13:17Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-311f50d89786406e90cffb0e6b0783922023-09-02T22:58:30ZengMDPI AGCancers2072-66942019-05-0111572510.3390/cancers11050725cancers11050725Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian PatientsTegshee Tserentogtokh0Boldbaatar Gantuya1Phawinee Subsomwong2Khasag Oyuntsetseg3Dashdorj Bolor4Yansan Erdene-Ochir5Dashdorj Azzaya6Duger Davaadorj7Tomohisa Uchida8Takeshi Matsuhisa9Yoshio Yamaoka10Department of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar city 14210, MongoliaDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar city 14210, MongoliaDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu city 879-5593, JapanDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar city 14210, MongoliaDepartment of Endoscopy, National Cancer Center, Ulaanbaatar city 13370, MongoliaDepartment of General Surgery National Cancer Center, Ulaanbaatar city 13370, MongoliaDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu city 879-5593, JapanDepartment of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar city 14210, MongoliaDepartment of Molecular Pathology, Oita University Faculty of Medicine, Yufu city 879-5593, JapanDepartment of Endoscopy, Nippon Medical University Tama Nagayama Hospital, Tokyo 113-8602, JapanDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu city 879-5593, Japan<i>Helicobacter pylori</i> infection possessing East-Asian-type CagA is associated with carcinogenesis. Mongolia has the highest mortality rate from gastric cancer. Therefore, we evaluated the CagA status in the Mongolian population. High risk and gastric cancer patients were determined using endoscopy and histological examination. <i>H. pylori</i> strains were isolated from different locations in Mongolia. The CagA subtypes (East-Asian-type or Western-type, based on sequencing of Glu-Pro-Ile-Tyr-Ala (EPIYA) segments) and <i>vacA</i> genotypes (s and m regions) were determined using PCR-based sequencing and PCR, respectively. In total, 368 patients were examined (341 gastritis, 10 peptic ulcer, and 17 gastric cancer). Sixty-two (16.8%) strains were <i>cagA</i>-negative and 306 (83.1%) were <i>cagA</i>-positive (293 Western-type, 12 East-Asian-type, and one hybrid type). All <i>cagA</i>-negative strains were isolated from gastritis patients. In the gastritis group, 78.6% (268/341) had Western-type CagA, 2.9% (10/341) had East-Asian-type, and 18.2% (61/341) were <i>cagA</i>-negative. However, all <i>H. pylori</i> from gastric cancer patients possessed Western-type CagA. Histological analyses showed that East-Asian-type CagA was the most virulent strains, followed by Western-type and <i>cagA</i>-negative strains. This finding agreed with the current consensus. <i>CagA</i>-positive strains were the most virulent type. However, the fact that different CagA types can explain the high incidence of gastric cancer might be inapplicable in Mongolia.https://www.mdpi.com/2072-6694/11/5/725<i>Helicobacter pylori</i>CagAVacAgenotypinggastric cancerMongolia |
| spellingShingle | Tegshee Tserentogtokh Boldbaatar Gantuya Phawinee Subsomwong Khasag Oyuntsetseg Dashdorj Bolor Yansan Erdene-Ochir Dashdorj Azzaya Duger Davaadorj Tomohisa Uchida Takeshi Matsuhisa Yoshio Yamaoka Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients Cancers <i>Helicobacter pylori</i> CagA VacA genotyping gastric cancer Mongolia |
| title | Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients |
| title_full | Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients |
| title_fullStr | Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients |
| title_full_unstemmed | Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients |
| title_short | Western-Type <i>Helicobacter pylori</i> CagA are the Most Frequent Type in Mongolian Patients |
| title_sort | western type i helicobacter pylori i caga are the most frequent type in mongolian patients |
| topic | <i>Helicobacter pylori</i> CagA VacA genotyping gastric cancer Mongolia |
| url | https://www.mdpi.com/2072-6694/11/5/725 |
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