Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)

Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)

Abstract Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared. Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeti...

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Bibliographic Details
Main Authors: Seinen Chow, Nobuaki Suzuki, Yoji Nakamura, Motoshige Yasuike, Kenji Saitoh, Takashi Yanagimoto
Format: Article
Language:English
Published: Islamic Azad University​ 2017-01-01
Series:International Aquatic Research
Subjects:
Thunnus orientalis
Four calmodulin genes
Locus-specific primers
Cross-species amplification
DNA marker
Online Access:http://link.springer.com/article/10.1007/s40071-017-0155-4
Description
Summary:Abstract Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared. Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeting introns were designed. These primer pairs amplified expected size of fragments in five congeneric tuna species, and utility of these primer pairs was investigated using distant fish species.
ISSN:2008-4935
2008-6970

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