High-Resolution Bioassay Profiling with Complemented Sensitivity and Resolution for Pancreatic Lipase Inhibitor Screening

High-Resolution Bioassay Profiling with Complemented Sensitivity and Resolution for Pancreatic Lipase Inhibitor Screening

How to rapidly and accurately screen bioactive components from complex natural products remains a major challenge. In this study, a screening platform for pancreatic lipase (PL) inhibitors was established by combining magnetic beads-based ligand fishing and high-resolution bioassay profiling. This p...

Full description

Bibliographic Details
Main Authors:	Jingyi Jian, Jiaming Yuan, Yu Fan, Jincai Wang, Tingting Zhang, Jeroen Kool, Zhengjin Jiang
Format:	Article
Language:	English
Published:	MDPI AG 2022-10-01
Series:	Molecules
Subjects:	high-resolution bioassay profiling magnetic beads-based ligand fishing high-performance liquid chromatography pancreatic lipase inhibitor screening
Online Access:	https://www.mdpi.com/1420-3049/27/20/6923

Similar Items

Enantioselective Chromatographic Separation and Lipase Catalyzed Asymmetric Resolution of Biologically Important Chiral Amines
by: Mohammed Farrag El-Behairy, et al.
Published: (2021-09-01)

Resolution of Racemic Acids, Esters and Amines by Candida rugosa Lipase in Slightly Hydrated Organic Media
by: Andrés R. Alcántara, et al.
Published: (2004-01-01)

Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review
by: Miroslav Pohanka
Published: (2019-02-01)

Lipase-Catalyzed Kinetic Resolution of Aryltrimethylsilyl Chiral Alcohols
by: Leandro H. Andrade, et al.
Published: (2011-11-01)

Role of amylase and lipase in diagnosis of acute pancreatitis
by: Lavanya, et al.
Published: (2018-01-01)

Evaluation of pancreatic lipase and gamma-glutamyl transferase in acute pancreatitis
by: Ahmed Abdulkadir Baban
Published: (2020-12-01)

Progression of lipase activity and pancreatic lipase immunoreactivity in dogs hospitalized for acute pancreatitis and correlation with clinical features
by: Claudia Cueni, et al.
Published: (2023-01-01)

Rapid Screening of Lipase Inhibitors in <em>Scutellaria</em><em> baicalensis</em> by Using Porcine Pancreatic Lipase Immobilized on Magnetic Core–Shell Metal–Organic Frameworks
by: Jinfang Xu, et al.
Published: (2022-05-01)

Solvent effect in lipase-catalyzed racemate resolution/
by: 241971 Anthonsen, Thorleif, et al.

Kinetic resolution of secondary alcohols with commercial lipases/
by: 394832 Sonnet, Philip E., et al.

Pancreatic lipase inhibitory activity of selected pharmaceutical agents
by: Hamdan Imad I., et al.
Published: (2019-03-01)

Efficient screening of pancreatic lipase inhibitors from cod meat hydrolysate through ligand fishing strategy
by: Yongqi Tian, et al.
Published: (2022-08-01)

Enantiodivergent Synthesis of Halofuginone by Candida antarctica Lipase B (CAL-B)-Catalyzed Kinetic Resolution in Cyclopentyl Methyl Ether (CPME)
by: Elisa De Marchi, et al.
Published: (2021-06-01)

Progress on the Stereoselective Synthesis of Chiral Molecules Based on Metal-Catalyzed Dynamic Kinetic Resolution of Alcohols with Lipases
by: Raffaella Ferraccioli
Published: (2021-09-01)

Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
by: Josilene Chaves Ruela Corrêa, et al.
Published: (2012-01-01)

Kinetic resolution of amino acid esters catalyzed by lipases/
by: 372796 Houng, J.

Preoperative assessment of peripheral vascular invasion of pancreatic ductal adenocarcinoma based on high-resolution MRI
by: Xiaoqi Zhou, et al.
Published: (2023-11-01)

Lipase activity in the human aorta
by: Kiyoshi Hayase, et al.
Published: (1970-05-01)

Stereoselective Synthesis of Terpenoids through Lipase-Mediated Resolution Approaches
by: Stefano Serra, et al.
Published: (2020-05-01)

Chalcones as Potential Inhibitors of Pancreatic Lipase
by: Sílvia Rocha, et al.
Published: (2022-11-01)

Targeted Selecting of Bioassay Methods as an Alternative to the “Battery of Bioassays”
by: Anna Olkova
Published: (2022-03-01)

Screening of supports for immobilization of commercial porcine pancreatic lipase
by: Robison Scherer, et al.
Published: (2011-12-01)

Structure and Function of Pancreatic Lipase-Related Protein 2 and Its Relationship With Pathological States
by: Guoying Zhu, et al.
Published: (2021-07-01)

Screening for pancreatic lipase inhibitors: evaluating assay conditions using p-nitrophenyl palmitate as substrate
by: Cam-Van T. Vo, et al.
Published: (2022-12-01)

Correlation between Levels of Serum Amylase, Lipase and Triglyceride in Acute Pancreatitis Patients
by: Gunalan Govindarajan, et al.
Published: (2015-03-01)

Functional Imaging and Inhibitor Screening of Human Pancreatic Lipase by a Resorufin-Based Fluorescent Probe
by: Fan-Bin Hou, et al.
Published: (2023-02-01)

Lipase-Catalyzed Kinetic Resolution of Alcohols as Intermediates for the Synthesis of Heart Rate Reducing Agent Ivabradine
by: Anna Morandini, et al.
Published: (2021-01-01)

Relação lipase/amilase nas pancreatites agudas de causa biliar e nas pancreatites agudas/crônicas agudizadas de causa alcoólica Lipase/amylase ratio in biliary acute pancreatitis and alcoholic acute/acutized chronic pancreatitis
by: Ricardo Custódio Pacheco, et al.
Published: (2007-03-01)

Audit of amylase and lipase requests in suspected acute pancreatitis and cost implications, South Africa
by: Annie E. Cook, et al.
Published: (2022-09-01)

Serum amylase and lipase levels for prediction of postendoscopic retrograde cholangiopancreatography pancreatitis
by: Mohammad Minakari, et al.
Published: (2018-01-01)

Chemoenzymatic Stereoselective Synthesis of <i>trans</i>-Flavan-4-ols via Lipase-Catalyzed Kinetic Resolutions
by: Martín Soto, et al.
Published: (2021-10-01)

Raised Amylase/Lipase levels in Enteric Fever: Prognostic marker or a sign of Pancreatitis? – Case Report
by: Mayank Kapoor, et al.
Published: (2021-01-01)

Histopathological findings and canine pancreatic lipase immunoreactivity in normal dogs and dogs with inflammatory and neoplastic diseases of the pancreas
by: Heike Aupperle‐Lellbach, et al.
Published: (2020-05-01)

Retrospective Analysis of Acute Pancreatitis Cases: Diagnostic accuracy of Amylase or Lipase Alone
by: Gülşah Çıkrıkçı Işık, et al.
Published: (2021-03-01)

Exploring Aurone Derivatives as Potential Human Pancreatic Lipase Inhibitors through Molecular Docking and Molecular Dynamics Simulations
by: Phuong Thuy Viet Nguyen, et al.
Published: (2020-10-01)

Effective Biodegradation of Mycotoxin Patulin by Porcine Pancreatic Lipase
by: Bingjie Liu, et al.
Published: (2018-04-01)

Surface display of pancreatic lipase inhibitor peptides by engineered Saccharomyces boulardii: Potential as an anti-obesity probiotic
by: Mahsa Nayebhashemi, et al.
Published: (2023-03-01)

Immobilization of Thermomyces lanuginosus lipase through isocyanide-based multi component reaction on multi-walled carbon nanotube: application for kinetic resolution of rac-ibuprofen
by: Mohamad Reza Safarpoor Moguei, et al.
Published: (2022-09-01)

New Applications of High-Resolution Analytical Methods to Study Trace Organic Compounds in Extraterrestrial Materials
by: Hiroshi Naraoka, et al.
Published: (2019-07-01)

Resolution of D, L-menthol by interesterification with triacetin using the free and immobilized lipase of candida sylindracea /
by: 325836 Lokotsch, Wolfram, et al.