The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction
The essential components of splicing are the splicing factors accumulated in nuclear speckles; thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (<i>poly (ADP-ribose) pol...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/2/297 |
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| author | Soňa Legartová Paolo Fagherazzi Lenka Stixová Aleš Kovařík Ivan Raška Eva Bártová |
| author_facet | Soňa Legartová Paolo Fagherazzi Lenka Stixová Aleš Kovařík Ivan Raška Eva Bártová |
| author_sort | Soňa Legartová |
| collection | DOAJ |
| description | The essential components of splicing are the splicing factors accumulated in nuclear speckles; thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (<i>poly (ADP-ribose) polymerase</i>), and more pronouncedly inhibitors of RNA polymerases, caused DNA damage and increased the SC-35 protein level. Interestingly, nuclear blebs, induced by PARP inhibitor and observed in A-type lamin-depleted or senescent cells, were positive on both the SC-35 protein and another component of the spliceosome, SRRM2. In the interphase cell nuclei, SC-35 interacted with the phosphorylated form of RNAP II, which was A-type lamin-dependent. In mitotic cells, especially in telophase, the SC-35 protein formed a well-visible ring in the cytoplasmic fraction and colocalized with β-catenin, associated with the plasma membrane. The antibody against the SRRM2 protein showed that nuclear speckles are already established in the cytoplasm of the late telophase and at the stage of early cytokinesis. In addition, we observed the occurrence of splicing factors in the nuclear blebs and micronuclei, which are also sites of both transcription and splicing. This conclusion supports the fact that splicing proceeds transcriptionally. According to our data, this process is A-type lamin-dependent. Lamin depletion also reduces the interaction between SC-35 and β-catenin in mitotic cells. |
| first_indexed | 2024-03-09T06:11:21Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T06:11:21Z |
| publishDate | 2021-02-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-3128938e4e7444a7a59911331cdba6df2023-12-03T11:57:48ZengMDPI AGCells2073-44092021-02-0110229710.3390/cells10020297The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This InteractionSoňa Legartová0Paolo Fagherazzi1Lenka Stixová2Aleš Kovařík3Ivan Raška4Eva Bártová5Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech RepublicInstitute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech RepublicInstitute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech RepublicInstitute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic1st Faculty of Medicine, Charles University, Albertov 4, 128 00 Praha, Czech RepublicInstitute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech RepublicThe essential components of splicing are the splicing factors accumulated in nuclear speckles; thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (<i>poly (ADP-ribose) polymerase</i>), and more pronouncedly inhibitors of RNA polymerases, caused DNA damage and increased the SC-35 protein level. Interestingly, nuclear blebs, induced by PARP inhibitor and observed in A-type lamin-depleted or senescent cells, were positive on both the SC-35 protein and another component of the spliceosome, SRRM2. In the interphase cell nuclei, SC-35 interacted with the phosphorylated form of RNAP II, which was A-type lamin-dependent. In mitotic cells, especially in telophase, the SC-35 protein formed a well-visible ring in the cytoplasmic fraction and colocalized with β-catenin, associated with the plasma membrane. The antibody against the SRRM2 protein showed that nuclear speckles are already established in the cytoplasm of the late telophase and at the stage of early cytokinesis. In addition, we observed the occurrence of splicing factors in the nuclear blebs and micronuclei, which are also sites of both transcription and splicing. This conclusion supports the fact that splicing proceeds transcriptionally. According to our data, this process is A-type lamin-dependent. Lamin depletion also reduces the interaction between SC-35 and β-catenin in mitotic cells.https://www.mdpi.com/2073-4409/10/2/297splicingSC-35PARP inhibitorRNA pol II |
| spellingShingle | Soňa Legartová Paolo Fagherazzi Lenka Stixová Aleš Kovařík Ivan Raška Eva Bártová The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction Cells splicing SC-35 PARP inhibitor RNA pol II |
| title | The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction |
| title_full | The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction |
| title_fullStr | The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction |
| title_full_unstemmed | The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction |
| title_short | The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction |
| title_sort | sc 35 splicing factor interacts with rna pol ii and a type lamin depletion weakens this interaction |
| topic | splicing SC-35 PARP inhibitor RNA pol II |
| url | https://www.mdpi.com/2073-4409/10/2/297 |
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