A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification
Summary: Based on high-throughput transcriptomic sequencing, SNHG3 was among the most highly expressed long noncoding RNAs in calcific aortic valve disease. SNHG3 upregulation was verified in human and mouse calcified aortic valves. Moreover, in vivo and in vitro studies showed SNHG3 silencing marke...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | JACC: Basic to Translational Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452302X22002297 |
| _version_ | 1811208535105601536 |
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| author | Long Chen, MD Hanning Liu, MD, PhD Cheng Sun, MD, PhD Jianqiu Pei, PhD Jun Li, MD Yue Li, PhD Ke Wei, MD, PhD Xiaoyi Wang, MD Peng Wang, MD Fangzhou Li, MD Shujie Gai, MD, PhD Yan Zhao, MD Zhe Zheng, MD, PhD |
| author_facet | Long Chen, MD Hanning Liu, MD, PhD Cheng Sun, MD, PhD Jianqiu Pei, PhD Jun Li, MD Yue Li, PhD Ke Wei, MD, PhD Xiaoyi Wang, MD Peng Wang, MD Fangzhou Li, MD Shujie Gai, MD, PhD Yan Zhao, MD Zhe Zheng, MD, PhD |
| author_sort | Long Chen, MD |
| collection | DOAJ |
| description | Summary: Based on high-throughput transcriptomic sequencing, SNHG3 was among the most highly expressed long noncoding RNAs in calcific aortic valve disease. SNHG3 upregulation was verified in human and mouse calcified aortic valves. Moreover, in vivo and in vitro studies showed SNHG3 silencing markedly ameliorated aortic valve calcification. In-depth functional assays showed SNHG3 physically interacted with polycomb repressive complex 2 to suppress the H3K27 trimethylation BMP2 locus, which in turn activated BMP2 expression and signaling pathways. Taken together, SNHG3 promoted aortic valve calcification by upregulating BMP2, which might be a novel therapeutic target in human calcific aortic valve disease. |
| first_indexed | 2024-04-12T04:24:14Z |
| format | Article |
| id | doaj.art-3129fe2e76f44c7a89427c5cf1419cbe |
| institution | Directory Open Access Journal |
| issn | 2452-302X |
| language | English |
| last_indexed | 2024-04-12T04:24:14Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JACC: Basic to Translational Science |
| spelling | doaj.art-3129fe2e76f44c7a89427c5cf1419cbe2022-12-22T03:48:09ZengElsevierJACC: Basic to Translational Science2452-302X2022-09-0179899914A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve CalcificationLong Chen, MD0Hanning Liu, MD, PhD1Cheng Sun, MD, PhD2Jianqiu Pei, PhD3Jun Li, MD4Yue Li, PhD5Ke Wei, MD, PhD6Xiaoyi Wang, MD7Peng Wang, MD8Fangzhou Li, MD9Shujie Gai, MD, PhD10Yan Zhao, MD11Zhe Zheng, MD, PhD12Department of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaDepartment of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaAddress for correspondence: Dr Zhe Zheng, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 167 Beilishi Road, Xicheng District, Beijing, PR China.; Department of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Disease, China and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of ChinaSummary: Based on high-throughput transcriptomic sequencing, SNHG3 was among the most highly expressed long noncoding RNAs in calcific aortic valve disease. SNHG3 upregulation was verified in human and mouse calcified aortic valves. Moreover, in vivo and in vitro studies showed SNHG3 silencing markedly ameliorated aortic valve calcification. In-depth functional assays showed SNHG3 physically interacted with polycomb repressive complex 2 to suppress the H3K27 trimethylation BMP2 locus, which in turn activated BMP2 expression and signaling pathways. Taken together, SNHG3 promoted aortic valve calcification by upregulating BMP2, which might be a novel therapeutic target in human calcific aortic valve disease.http://www.sciencedirect.com/science/article/pii/S2452302X22002297BMP2 pathwaycalcific aortic valve diseaselong noncoding RNAosteoblast differentiationtherapeutic target |
| spellingShingle | Long Chen, MD Hanning Liu, MD, PhD Cheng Sun, MD, PhD Jianqiu Pei, PhD Jun Li, MD Yue Li, PhD Ke Wei, MD, PhD Xiaoyi Wang, MD Peng Wang, MD Fangzhou Li, MD Shujie Gai, MD, PhD Yan Zhao, MD Zhe Zheng, MD, PhD A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification JACC: Basic to Translational Science BMP2 pathway calcific aortic valve disease long noncoding RNA osteoblast differentiation therapeutic target |
| title | A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification |
| title_full | A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification |
| title_fullStr | A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification |
| title_full_unstemmed | A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification |
| title_short | A Novel LncRNA SNHG3 Promotes Osteoblast Differentiation Through BMP2 Upregulation in Aortic Valve Calcification |
| title_sort | novel lncrna snhg3 promotes osteoblast differentiation through bmp2 upregulation in aortic valve calcification |
| topic | BMP2 pathway calcific aortic valve disease long noncoding RNA osteoblast differentiation therapeutic target |
| url | http://www.sciencedirect.com/science/article/pii/S2452302X22002297 |
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