Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats
Gentamicin is an aminoglycoside antibiotic commonly used to treat Gram-negative bacterial infections that possesses considerable nephrotoxicity. Oxymatrine is a phytochemical with the ability to counter gentamicin toxicity. We investigated the effects and protective mechanism of oxymatrine in rats....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/19/6209 |
| _version_ | 1797478039963041792 |
|---|---|
| author | Songyao Kang Tingting Chen Zhihui Hao Xiao Yang Mingfa Wang Zhifang Zhang Sijia Hao Fengting Lang Hongxia Hao |
| author_facet | Songyao Kang Tingting Chen Zhihui Hao Xiao Yang Mingfa Wang Zhifang Zhang Sijia Hao Fengting Lang Hongxia Hao |
| author_sort | Songyao Kang |
| collection | DOAJ |
| description | Gentamicin is an aminoglycoside antibiotic commonly used to treat Gram-negative bacterial infections that possesses considerable nephrotoxicity. Oxymatrine is a phytochemical with the ability to counter gentamicin toxicity. We investigated the effects and protective mechanism of oxymatrine in rats. The experimental groups were as follows: Control, Oxymatrine only group (100 mg/kg/d), Gentamicin only group (100 mg/kg/d), Gentamicin (100 mg/kg/d) plus Oxymatrine (100 mg/kg/d) group (<i>n</i> = 10). All rats were treated for seven continuous days. The results indicated that oxymatrine alleviated gentamicin-induced kidney injury, and decreased rats’ kidney indices and NAG (<i>N</i>-acetyl-beta-<span style="font-variant: small-caps;">d</span>-glucosaminidase), BUN (blood urea nitrogen) and CRE (creatine) serum levels. The oxymatrine-treated group sustained less histological damage. Oxymatrine also relived gentamicin-induced oxidative and nitrative stress, indicated by the increased SOD (superoxidase dismutase), GSH (glutathione) and CAT (catalase) activities and decreased MDA (malondialdehyde), iNOS (inducible nitric oxide synthase) and NO (nitric oxide) levels. Caspase-9 and -3 activities were also decreased in the oxymatrine-treated group. Oxymatrine exhibited a potent anti-inflammatory effect on gentamicin-induced kidney injury, down-regulated the Bcl-2ax and NF-κB mRNAs, and upregulated Bcl-2, HO-1 and Nrf2 mRNAs in the kidney tissue. Our investigation revealed the renal protective effect of oxymatrine in gentamicin-induced kidney injury for the first time. The effect was achieved through activation of the Nrf2/HO-1 pathways. The study underlines the potential clinical application of oxymatrine as a renal protectant agent for gentamicin therapy. |
| first_indexed | 2024-03-09T21:26:22Z |
| format | Article |
| id | doaj.art-312aa77eca7e49068e12e142d1990790 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:26:22Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-312aa77eca7e49068e12e142d19907902023-11-23T21:07:26ZengMDPI AGMolecules1420-30492022-09-012719620910.3390/molecules27196209Oxymatrine Alleviates Gentamicin-Induced Renal Injury in RatsSongyao Kang0Tingting Chen1Zhihui Hao2Xiao Yang3Mingfa Wang4Zhifang Zhang5Sijia Hao6Fengting Lang7Hongxia Hao8Chinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaChinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaChinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaChinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaQingdao University Medical Group Juxian Hospital, Qingdao 276599, ChinaCollege of Traditional Medicine, Inner Mongolia Medical University, Hohhot 010059, ChinaCollege of Traditional Medicine, Inner Mongolia Medical University, Hohhot 010059, ChinaChinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaChinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaGentamicin is an aminoglycoside antibiotic commonly used to treat Gram-negative bacterial infections that possesses considerable nephrotoxicity. Oxymatrine is a phytochemical with the ability to counter gentamicin toxicity. We investigated the effects and protective mechanism of oxymatrine in rats. The experimental groups were as follows: Control, Oxymatrine only group (100 mg/kg/d), Gentamicin only group (100 mg/kg/d), Gentamicin (100 mg/kg/d) plus Oxymatrine (100 mg/kg/d) group (<i>n</i> = 10). All rats were treated for seven continuous days. The results indicated that oxymatrine alleviated gentamicin-induced kidney injury, and decreased rats’ kidney indices and NAG (<i>N</i>-acetyl-beta-<span style="font-variant: small-caps;">d</span>-glucosaminidase), BUN (blood urea nitrogen) and CRE (creatine) serum levels. The oxymatrine-treated group sustained less histological damage. Oxymatrine also relived gentamicin-induced oxidative and nitrative stress, indicated by the increased SOD (superoxidase dismutase), GSH (glutathione) and CAT (catalase) activities and decreased MDA (malondialdehyde), iNOS (inducible nitric oxide synthase) and NO (nitric oxide) levels. Caspase-9 and -3 activities were also decreased in the oxymatrine-treated group. Oxymatrine exhibited a potent anti-inflammatory effect on gentamicin-induced kidney injury, down-regulated the Bcl-2ax and NF-κB mRNAs, and upregulated Bcl-2, HO-1 and Nrf2 mRNAs in the kidney tissue. Our investigation revealed the renal protective effect of oxymatrine in gentamicin-induced kidney injury for the first time. The effect was achieved through activation of the Nrf2/HO-1 pathways. The study underlines the potential clinical application of oxymatrine as a renal protectant agent for gentamicin therapy.https://www.mdpi.com/1420-3049/27/19/6209gentamicinnephrotoxicityoxymatrineoxidative stressNrf2/HO-1 |
| spellingShingle | Songyao Kang Tingting Chen Zhihui Hao Xiao Yang Mingfa Wang Zhifang Zhang Sijia Hao Fengting Lang Hongxia Hao Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats Molecules gentamicin nephrotoxicity oxymatrine oxidative stress Nrf2/HO-1 |
| title | Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats |
| title_full | Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats |
| title_fullStr | Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats |
| title_full_unstemmed | Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats |
| title_short | Oxymatrine Alleviates Gentamicin-Induced Renal Injury in Rats |
| title_sort | oxymatrine alleviates gentamicin induced renal injury in rats |
| topic | gentamicin nephrotoxicity oxymatrine oxidative stress Nrf2/HO-1 |
| url | https://www.mdpi.com/1420-3049/27/19/6209 |
| work_keys_str_mv | AT songyaokang oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT tingtingchen oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT zhihuihao oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT xiaoyang oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT mingfawang oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT zhifangzhang oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT sijiahao oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT fengtinglang oxymatrinealleviatesgentamicininducedrenalinjuryinrats AT hongxiahao oxymatrinealleviatesgentamicininducedrenalinjuryinrats |