Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry
Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera <i>Staphylococcus, Lactococcus, Enterococcus, Microbacteri...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/21/5143 |
| _version_ | 1797548796931997696 |
|---|---|
| author | Petra Jančová Vendula Pachlová Erika Čechová Karolína Cedidlová Jana Šerá Hana Pištěková František Buňka Leona Buňková |
| author_facet | Petra Jančová Vendula Pachlová Erika Čechová Karolína Cedidlová Jana Šerá Hana Pištěková František Buňka Leona Buňková |
| author_sort | Petra Jančová |
| collection | DOAJ |
| description | Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera <i>Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella</i> and <i>Enterobacter</i> were identified by the MALDI-TOF MS and biochemical methods. The <i>in vitro</i> produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV–Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L<sup>−1</sup>. Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored. |
| first_indexed | 2024-03-10T15:04:52Z |
| format | Article |
| id | doaj.art-312aad27f3b0488580df20b7f4cd2161 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:04:52Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-312aad27f3b0488580df20b7f4cd21612023-11-20T19:50:20ZengMDPI AGMolecules1420-30492020-11-012521514310.3390/molecules25215143Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy IndustryPetra Jančová0Vendula Pachlová1Erika Čechová2Karolína Cedidlová3Jana Šerá4Hana Pištěková5František Buňka6Leona Buňková7Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicDepartment of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicDepartment of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicDepartment of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicDepartment of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicDepartment of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicFood Research Laboratory, Department of Logistics, Faculty of Military Leadership, University of Defence, Kounicova 65, 662 10 Brno, Czech RepublicDepartment of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 760 01 Zlín, Czech RepublicOut of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera <i>Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella</i> and <i>Enterobacter</i> were identified by the MALDI-TOF MS and biochemical methods. The <i>in vitro</i> produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV–Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L<sup>−1</sup>. Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored.https://www.mdpi.com/1420-3049/25/21/5143wastewaterbacteriadecarboxylase activitybiogenic amines |
| spellingShingle | Petra Jančová Vendula Pachlová Erika Čechová Karolína Cedidlová Jana Šerá Hana Pištěková František Buňka Leona Buňková Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry Molecules wastewater bacteria decarboxylase activity biogenic amines |
| title | Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry |
| title_full | Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry |
| title_fullStr | Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry |
| title_full_unstemmed | Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry |
| title_short | Occurrence of Biogenic Amines Producers in the Wastewater of the Dairy Industry |
| title_sort | occurrence of biogenic amines producers in the wastewater of the dairy industry |
| topic | wastewater bacteria decarboxylase activity biogenic amines |
| url | https://www.mdpi.com/1420-3049/25/21/5143 |
| work_keys_str_mv | AT petrajancova occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT vendulapachlova occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT erikacechova occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT karolinacedidlova occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT janasera occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT hanapistekova occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT frantisekbunka occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry AT leonabunkova occurrenceofbiogenicaminesproducersinthewastewaterofthedairyindustry |