Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain
Yellow mealworm (Tenebrio molitor) is a supplementary protein source for food and feed and represents a promising solution to manage grain contaminated with Aflatoxin B1 (AFB1). In this study, AFB1 present in different concentrations in wheat bran was treated and removed via bioconversion by yellow...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322007758 |
| _version_ | 1811214705072537600 |
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| author | Dandan Zhao Hui Xie Lei Gao Jian Zhang Yan Li Guotao Mao Hongsen Zhang Fengqin Wang Su Shiung Lam Andong Song |
| author_facet | Dandan Zhao Hui Xie Lei Gao Jian Zhang Yan Li Guotao Mao Hongsen Zhang Fengqin Wang Su Shiung Lam Andong Song |
| author_sort | Dandan Zhao |
| collection | DOAJ |
| description | Yellow mealworm (Tenebrio molitor) is a supplementary protein source for food and feed and represents a promising solution to manage grain contaminated with Aflatoxin B1 (AFB1). In this study, AFB1 present in different concentrations in wheat bran was treated and removed via bioconversion by yellow mealworm of different instars, with emphasis on the bioconversion performance and metabolism of AFB1. Upon application of wheat bran spiked with 100 μg/kg AFB1 to 5th–6th instar yellow mealworms, the conversion rate of AFB1 was up to 87.85 %. Low level of AFB1 (< 2 μg/kg) was accumulated in the larval bodies, and the survival rate, development and nutrition contents of yellow mealworm were not significantly affected. It was revealed that 1 kg of wheat bran contaminated with AFB1 increased the weight of yellow mealworms from 138 g to 469 g, containing approximately 103 g of protein. The bioconversion of AFB1 by yellow mealworms led to generation of 13 metabolites in the frass and 3 metabolites in the larvae. AFB1 was detoxicated and removed via phase I metabolism comprising reduction, dehydrogenation, hydration, demethylation, hydroxylation, decarbonylation and ketoreduction, followed by phase II metabolism involving conjugation of amino acid, glucoside and glutathione (GSH). The toxicity of AFB1 metabolites was deemed lower than that of AFB1 according to their structures. This study provides a sustainable approach and theoretical foundation on using yellow mealworms for cleaner grain contamination management and valuable larval protein production via bioconversion of food and feed contaminated by AFB1. |
| first_indexed | 2024-04-12T06:09:11Z |
| format | Article |
| id | doaj.art-3130e4c8817441bf8458e9e284fdf833 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T06:09:11Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-3130e4c8817441bf8458e9e284fdf8332022-12-22T03:44:45ZengElsevierEcotoxicology and Environmental Safety0147-65132022-09-01242113935Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grainDandan Zhao0Hui Xie1Lei Gao2Jian Zhang3Yan Li4Guotao Mao5Hongsen Zhang6Fengqin Wang7Su Shiung Lam8Andong Song9College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR China; The Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR ChinaSchool of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR China; The Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR China; The Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR China; The Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, PR ChinaPyrolysis Technology Research Group, Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Sustainability Cluster, School of Engineering, University of Petroleum & Energy Studies, Dehradun, Uttarakhand 248007, IndiaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR China; The Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, Henan Agricultural University, Zhengzhou 450002, PR China; Correspondence to: College of Life Science, Henan Agricultural University, Nongye Road No. 63, Zhengzhou 450002, China.Yellow mealworm (Tenebrio molitor) is a supplementary protein source for food and feed and represents a promising solution to manage grain contaminated with Aflatoxin B1 (AFB1). In this study, AFB1 present in different concentrations in wheat bran was treated and removed via bioconversion by yellow mealworm of different instars, with emphasis on the bioconversion performance and metabolism of AFB1. Upon application of wheat bran spiked with 100 μg/kg AFB1 to 5th–6th instar yellow mealworms, the conversion rate of AFB1 was up to 87.85 %. Low level of AFB1 (< 2 μg/kg) was accumulated in the larval bodies, and the survival rate, development and nutrition contents of yellow mealworm were not significantly affected. It was revealed that 1 kg of wheat bran contaminated with AFB1 increased the weight of yellow mealworms from 138 g to 469 g, containing approximately 103 g of protein. The bioconversion of AFB1 by yellow mealworms led to generation of 13 metabolites in the frass and 3 metabolites in the larvae. AFB1 was detoxicated and removed via phase I metabolism comprising reduction, dehydrogenation, hydration, demethylation, hydroxylation, decarbonylation and ketoreduction, followed by phase II metabolism involving conjugation of amino acid, glucoside and glutathione (GSH). The toxicity of AFB1 metabolites was deemed lower than that of AFB1 according to their structures. This study provides a sustainable approach and theoretical foundation on using yellow mealworms for cleaner grain contamination management and valuable larval protein production via bioconversion of food and feed contaminated by AFB1.http://www.sciencedirect.com/science/article/pii/S0147651322007758Aflatoxin B1Tenebrio molitorBioconversionDetoxicationMetabolic pathwayLarval protein |
| spellingShingle | Dandan Zhao Hui Xie Lei Gao Jian Zhang Yan Li Guotao Mao Hongsen Zhang Fengqin Wang Su Shiung Lam Andong Song Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain Ecotoxicology and Environmental Safety Aflatoxin B1 Tenebrio molitor Bioconversion Detoxication Metabolic pathway Larval protein |
| title | Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain |
| title_full | Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain |
| title_fullStr | Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain |
| title_full_unstemmed | Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain |
| title_short | Detoxication and bioconversion of aflatoxin B1 by yellow mealworms (Tenebrio molitor): A sustainable approach for valuable larval protein production from contaminated grain |
| title_sort | detoxication and bioconversion of aflatoxin b1 by yellow mealworms tenebrio molitor a sustainable approach for valuable larval protein production from contaminated grain |
| topic | Aflatoxin B1 Tenebrio molitor Bioconversion Detoxication Metabolic pathway Larval protein |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322007758 |
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