Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017
Background: Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the major causative agents of hand, foot and mouth disease (HFMD) worldwide, particularly in the Asia-Pacific region. Several strains have emerged, circulated, and faded out over time in recent decades. This study investigated...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-03-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971219300141 |
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| author | Pirom Noisumdaeng Achareeya Korkusol Jarunee Prasertsopon Kantima Sangsiriwut Kulkanya Chokephaibulkit Anek Mungaomklang Arunee Thitithanyanont Rome Buathong Ratigorn Guntapong Pilaipan Puthavathana |
| author_facet | Pirom Noisumdaeng Achareeya Korkusol Jarunee Prasertsopon Kantima Sangsiriwut Kulkanya Chokephaibulkit Anek Mungaomklang Arunee Thitithanyanont Rome Buathong Ratigorn Guntapong Pilaipan Puthavathana |
| author_sort | Pirom Noisumdaeng |
| collection | DOAJ |
| description | Background: Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the major causative agents of hand, foot and mouth disease (HFMD) worldwide, particularly in the Asia-Pacific region. Several strains have emerged, circulated, and faded out over time in recent decades. This study investigated the EV-A71 and CV-A16 circulating strains and replacement of genotypes/subgenotypes in Thailand during the years 2000–2017. Methods: The complete VP1 regions of 92 enteroviruses obtained from 90 HFMD patients, one asymptomatic adult contact case, and one encephalitic case were sequenced and investigated for serotypes, genotypes, and subgenotypes using a phylogenetic analysis. Results: The 92 enterovirus isolates were identified as 67 (72.8%) EV-A71 strains comprising subgenotypes B4, B5, C1, C2, C4a, C4b and C5, and 25 (27.2%) CV-A16 strains comprising subgenotypes B1a and B1b. Genotypic/subgenotypic replacements were evidenced during the study period. EV-A71 B5 and C4a have been the major circulating strains in Thailand for more than a decade, and CV-A16 B1a has been circulating for almost two decades. Conclusions: This study provides chronological data on the molecular epidemiology of EV-A71 and CV-A16 subgenotypes in Thailand. Subgenotypic replacement frequently occurred with EV-A71, but not CV-A16. Monitoring for viral genetic and subgenotypic changes is important for molecular diagnosis, vaccine selection, and vaccine development. Keywords: Hand, foot and mouth disease, Enterovirus A71, Coxsackievirus A16, VP1 sequence, Molecular epidemiology, Genotypic/subgenotypic replacement |
| first_indexed | 2024-12-21T06:33:36Z |
| format | Article |
| id | doaj.art-3133b11f5a0740a5bdc3225c6efcd96b |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-21T06:33:36Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-3133b11f5a0740a5bdc3225c6efcd96b2022-12-21T19:12:56ZengElsevierInternational Journal of Infectious Diseases1201-97122019-03-01808491Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017Pirom Noisumdaeng0Achareeya Korkusol1Jarunee Prasertsopon2Kantima Sangsiriwut3Kulkanya Chokephaibulkit4Anek Mungaomklang5Arunee Thitithanyanont6Rome Buathong7Ratigorn Guntapong8Pilaipan Puthavathana9Faculty of Public Health, Thammasat University (Rangsit Center), Khlong Luang, Pathum Thani 12121, ThailandDepartment of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, ThailandCenter for Research and Innovation, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, ThailandDepartment of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, ThailandDepartment of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, ThailandDebaratana Nakhon Ratchasima Hospital, Ministry of Public Health, Nakhon Ratchasima 30280, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandBureau of Epidemiology, Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, ThailandNational Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandDepartment of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noi, Bangkok 10700, Thailand; Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand; Corresponding author at: Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand.Background: Enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16) are the major causative agents of hand, foot and mouth disease (HFMD) worldwide, particularly in the Asia-Pacific region. Several strains have emerged, circulated, and faded out over time in recent decades. This study investigated the EV-A71 and CV-A16 circulating strains and replacement of genotypes/subgenotypes in Thailand during the years 2000–2017. Methods: The complete VP1 regions of 92 enteroviruses obtained from 90 HFMD patients, one asymptomatic adult contact case, and one encephalitic case were sequenced and investigated for serotypes, genotypes, and subgenotypes using a phylogenetic analysis. Results: The 92 enterovirus isolates were identified as 67 (72.8%) EV-A71 strains comprising subgenotypes B4, B5, C1, C2, C4a, C4b and C5, and 25 (27.2%) CV-A16 strains comprising subgenotypes B1a and B1b. Genotypic/subgenotypic replacements were evidenced during the study period. EV-A71 B5 and C4a have been the major circulating strains in Thailand for more than a decade, and CV-A16 B1a has been circulating for almost two decades. Conclusions: This study provides chronological data on the molecular epidemiology of EV-A71 and CV-A16 subgenotypes in Thailand. Subgenotypic replacement frequently occurred with EV-A71, but not CV-A16. Monitoring for viral genetic and subgenotypic changes is important for molecular diagnosis, vaccine selection, and vaccine development. Keywords: Hand, foot and mouth disease, Enterovirus A71, Coxsackievirus A16, VP1 sequence, Molecular epidemiology, Genotypic/subgenotypic replacementhttp://www.sciencedirect.com/science/article/pii/S1201971219300141 |
| spellingShingle | Pirom Noisumdaeng Achareeya Korkusol Jarunee Prasertsopon Kantima Sangsiriwut Kulkanya Chokephaibulkit Anek Mungaomklang Arunee Thitithanyanont Rome Buathong Ratigorn Guntapong Pilaipan Puthavathana Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017 International Journal of Infectious Diseases |
| title | Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017 |
| title_full | Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017 |
| title_fullStr | Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017 |
| title_full_unstemmed | Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017 |
| title_short | Longitudinal study on enterovirus A71 and coxsackievirus A16 genotype/subgenotype replacements in hand, foot and mouth disease patients in Thailand, 2000–2017 |
| title_sort | longitudinal study on enterovirus a71 and coxsackievirus a16 genotype subgenotype replacements in hand foot and mouth disease patients in thailand 2000 2017 |
| url | http://www.sciencedirect.com/science/article/pii/S1201971219300141 |
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