Endoglycan plays a role in axon guidance by modulating cell adhesion
Axon navigation depends on the interactions between guidance molecules along the trajectory and specific receptors on the growth cone. However, our in vitro and in vivo studies on the role of Endoglycan demonstrate that in addition to specific guidance cue – receptor interactions, axon guidance depe...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/64767 |
| _version_ | 1811181021969776640 |
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| author | Thomas Baeriswyl Alexandre Dumoulin Martina Schaettin Georgia Tsapara Vera Niederkofler Denise Helbling Evelyn Avilés Jeannine A Frei Nicole H Wilson Matthias Gesemann Beat Kunz Esther T Stoeckli |
| author_facet | Thomas Baeriswyl Alexandre Dumoulin Martina Schaettin Georgia Tsapara Vera Niederkofler Denise Helbling Evelyn Avilés Jeannine A Frei Nicole H Wilson Matthias Gesemann Beat Kunz Esther T Stoeckli |
| author_sort | Thomas Baeriswyl |
| collection | DOAJ |
| description | Axon navigation depends on the interactions between guidance molecules along the trajectory and specific receptors on the growth cone. However, our in vitro and in vivo studies on the role of Endoglycan demonstrate that in addition to specific guidance cue – receptor interactions, axon guidance depends on fine-tuning of cell-cell adhesion. Endoglycan, a sialomucin, plays a role in axon guidance in the central nervous system of chicken embryos, but it is neither an axon guidance cue nor a receptor. Rather, Endoglycan acts as a negative regulator of molecular interactions based on evidence from in vitro experiments demonstrating reduced adhesion of growth cones. In the absence of Endoglycan, commissural axons fail to properly navigate the midline of the spinal cord. Taken together, our in vivo and in vitro results support the hypothesis that Endoglycan acts as a negative regulator of cell-cell adhesion in commissural axon guidance. |
| first_indexed | 2024-04-11T09:11:21Z |
| format | Article |
| id | doaj.art-3135680495e24540ae49470517d4f7fe |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:11:21Z |
| publishDate | 2021-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3135680495e24540ae49470517d4f7fe2022-12-22T04:32:29ZengeLife Sciences Publications LtdeLife2050-084X2021-03-011010.7554/eLife.64767Endoglycan plays a role in axon guidance by modulating cell adhesionThomas Baeriswyl0Alexandre Dumoulin1https://orcid.org/0000-0002-2420-6877Martina Schaettin2Georgia Tsapara3Vera Niederkofler4Denise Helbling5Evelyn Avilés6Jeannine A Frei7Nicole H Wilson8Matthias Gesemann9Beat Kunz10Esther T Stoeckli11https://orcid.org/0000-0002-8485-0648Department of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandDepartment of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Zurich, SwitzerlandAxon navigation depends on the interactions between guidance molecules along the trajectory and specific receptors on the growth cone. However, our in vitro and in vivo studies on the role of Endoglycan demonstrate that in addition to specific guidance cue – receptor interactions, axon guidance depends on fine-tuning of cell-cell adhesion. Endoglycan, a sialomucin, plays a role in axon guidance in the central nervous system of chicken embryos, but it is neither an axon guidance cue nor a receptor. Rather, Endoglycan acts as a negative regulator of molecular interactions based on evidence from in vitro experiments demonstrating reduced adhesion of growth cones. In the absence of Endoglycan, commissural axons fail to properly navigate the midline of the spinal cord. Taken together, our in vivo and in vitro results support the hypothesis that Endoglycan acts as a negative regulator of cell-cell adhesion in commissural axon guidance.https://elifesciences.org/articles/64767sialomucincommissural axonslive imagingPodocalyxin-like 2floor plate |
| spellingShingle | Thomas Baeriswyl Alexandre Dumoulin Martina Schaettin Georgia Tsapara Vera Niederkofler Denise Helbling Evelyn Avilés Jeannine A Frei Nicole H Wilson Matthias Gesemann Beat Kunz Esther T Stoeckli Endoglycan plays a role in axon guidance by modulating cell adhesion eLife sialomucin commissural axons live imaging Podocalyxin-like 2 floor plate |
| title | Endoglycan plays a role in axon guidance by modulating cell adhesion |
| title_full | Endoglycan plays a role in axon guidance by modulating cell adhesion |
| title_fullStr | Endoglycan plays a role in axon guidance by modulating cell adhesion |
| title_full_unstemmed | Endoglycan plays a role in axon guidance by modulating cell adhesion |
| title_short | Endoglycan plays a role in axon guidance by modulating cell adhesion |
| title_sort | endoglycan plays a role in axon guidance by modulating cell adhesion |
| topic | sialomucin commissural axons live imaging Podocalyxin-like 2 floor plate |
| url | https://elifesciences.org/articles/64767 |
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