<i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway
Exposure to particulate matter (PM) has been known to be one of the risk factors to cause allergic asthma, leading to development of respiratory disease. <i>Banhahubak</i>-tang tablet (BHT), a standardized Korean Medicine, is prescribed for neurasthenia, laryngopharyngitis and asthma. In...
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/9/2206 |
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| author | Yeon Kyung Nam Seong Chul Jin Mi Hye Kim La Yoon Choi Yong-Bok Lee Woong Mo Yang |
| author_facet | Yeon Kyung Nam Seong Chul Jin Mi Hye Kim La Yoon Choi Yong-Bok Lee Woong Mo Yang |
| author_sort | Yeon Kyung Nam |
| collection | DOAJ |
| description | Exposure to particulate matter (PM) has been known to be one of the risk factors to cause allergic asthma, leading to development of respiratory disease. <i>Banhahubak</i>-tang tablet (BHT), a standardized Korean Medicine, is prescribed for neurasthenia, laryngopharyngitis and asthma. In this study, we investigated therapeutic effects of BHT on airway inflammation in ovalbumin (OVA) and PM smaller than 10 μm (PM<sub>10</sub>)-induced allergic asthma mice. To establish allergic asthma with airway hyper-responsiveness by PM<sub>10</sub>, BALB/c mice were sensitized and challenged with OVA and PM<sub>10</sub>, and orally administered BHT. Histological staining was performed to assess airway remodeling. Serum and bronchoalveolar lavage fluid (BALF) was collected for measuring immunoglobulin levels and counting inflammatory cells, respectively. Expression levels of Janus kinase 1 (JAK1)/signal transducer and activator of transcription 6 (STAT6), pro-inflammatory cytokines and type 2 T-helper (Th2)-related cytokines were analyzed in vivo and in vitro models. Histopathological analysis demonstrated that BHT suppressed inflammatory cell infiltration, mucus hypersecretion and collagen deposition in the airway. BHT administration effectively decreased number of inflammatory cells in BALF. BHT reduced total serum Immunoglobulin E (IgE) and Immunoglobulin G (IgG) levels. In addition, BHT significantly inhibited the phosphorylation of JAK1 and STAT6 expressions. Release of pro-inflammatory cytokines and Th2-related cytokines were down-regulated by BHT. In conclusion, BHT mitigated airway inflammation by down-regulating pro-inflammatory and Th2-related cytokines via JAK1/STAT6 signaling. BHT might be a promising herbal medicine for preventing airway inflammation. Moreover, an intervention study among humans is needed to further evaluate the possible beneficial effects of BHT in allergic asthma. |
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| language | English |
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| spelling | doaj.art-3136c9987a8f41faa6cce1e053ce54f82023-11-19T23:50:44ZengMDPI AGMolecules1420-30492020-05-01259220610.3390/molecules25092206<i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal PathwayYeon Kyung Nam0Seong Chul Jin1Mi Hye Kim2La Yoon Choi3Yong-Bok Lee4Woong Mo Yang5Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaCollege of Pharmacy, Chonnam National University, Gwangju 61186, KoreaDepartment of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaExposure to particulate matter (PM) has been known to be one of the risk factors to cause allergic asthma, leading to development of respiratory disease. <i>Banhahubak</i>-tang tablet (BHT), a standardized Korean Medicine, is prescribed for neurasthenia, laryngopharyngitis and asthma. In this study, we investigated therapeutic effects of BHT on airway inflammation in ovalbumin (OVA) and PM smaller than 10 μm (PM<sub>10</sub>)-induced allergic asthma mice. To establish allergic asthma with airway hyper-responsiveness by PM<sub>10</sub>, BALB/c mice were sensitized and challenged with OVA and PM<sub>10</sub>, and orally administered BHT. Histological staining was performed to assess airway remodeling. Serum and bronchoalveolar lavage fluid (BALF) was collected for measuring immunoglobulin levels and counting inflammatory cells, respectively. Expression levels of Janus kinase 1 (JAK1)/signal transducer and activator of transcription 6 (STAT6), pro-inflammatory cytokines and type 2 T-helper (Th2)-related cytokines were analyzed in vivo and in vitro models. Histopathological analysis demonstrated that BHT suppressed inflammatory cell infiltration, mucus hypersecretion and collagen deposition in the airway. BHT administration effectively decreased number of inflammatory cells in BALF. BHT reduced total serum Immunoglobulin E (IgE) and Immunoglobulin G (IgG) levels. In addition, BHT significantly inhibited the phosphorylation of JAK1 and STAT6 expressions. Release of pro-inflammatory cytokines and Th2-related cytokines were down-regulated by BHT. In conclusion, BHT mitigated airway inflammation by down-regulating pro-inflammatory and Th2-related cytokines via JAK1/STAT6 signaling. BHT might be a promising herbal medicine for preventing airway inflammation. Moreover, an intervention study among humans is needed to further evaluate the possible beneficial effects of BHT in allergic asthma.https://www.mdpi.com/1420-3049/25/9/2206allergic asthmaBHTPM<sub>10</sub>airway inflammationpro-inflammatory cytokineTh2-related cytokine |
| spellingShingle | Yeon Kyung Nam Seong Chul Jin Mi Hye Kim La Yoon Choi Yong-Bok Lee Woong Mo Yang <i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway Molecules allergic asthma BHT PM<sub>10</sub> airway inflammation pro-inflammatory cytokine Th2-related cytokine |
| title | <i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway |
| title_full | <i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway |
| title_fullStr | <i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway |
| title_full_unstemmed | <i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway |
| title_short | <i>Banhahubak</i>-Tang Tablet, a Standardized Medicine Attenuates Allergic Asthma via Inhibition of Janus Kinase 1 (JAK1)/ Signal Transducer and Activator of Transcription 6 (STAT6) Signal Pathway |
| title_sort | i banhahubak i tang tablet a standardized medicine attenuates allergic asthma via inhibition of janus kinase 1 jak1 signal transducer and activator of transcription 6 stat6 signal pathway |
| topic | allergic asthma BHT PM<sub>10</sub> airway inflammation pro-inflammatory cytokine Th2-related cytokine |
| url | https://www.mdpi.com/1420-3049/25/9/2206 |
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