Detection of Some Genes Related with Virulence Factors in the Clinical Isolates of Staphylococcus aureus with Potential Effects of Some Probiotics Lactobacillus Species
Staphylococcus aureus (S. aureus) is remarkable pathogen and causative agent of hospital acquired (nosocomial) and community acquired infections. S aureus can acquire resistance to most antimicrobial agents (antibiotics). A total 295 S aureus isolates were collected from two local hospitals from v...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2019-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/detection-of-some-genes-related-with-virulence-factors-in-the-clinical-isolates-of-staphylococcus-aureus-with-potential-effects-of-some-probiotics-lactobacillus-species/ |
| Summary: | Staphylococcus aureus (S. aureus) is remarkable pathogen and causative agent of hospital acquired
(nosocomial) and community acquired infections. S aureus can acquire resistance to most antimicrobial
agents (antibiotics). A total 295 S aureus isolates were collected from two local hospitals from various
sites of body patients, such as urine, pus, sputum, wound, and blood. Patients’ ages were ranging from
5-84 years from males and females. The number of identified S aureus was 83 detected by traditional
and methods and confirmed by molecular method using 16S rRNA gene in polymerase chain reaction
(PCR). Antibiotic sensitivity profile was performed to evaluate the resistant and sensitive strains and
predominance of methicillin resistant S aureus (MRSA). A total 6 (7.3%) from all S. aureus isolates
was MRSA. MRSA isolates were multi-resistant for all antibiotics, which used in the current study.
Several genes that participate in antibiotic resistance were investigated. SasX gene was detected in 11
(12.3%) samples which was multi-resistant for many antibiotics. In addition, Furthermore, Exfoliative
toxin C and D (etC and etD) genes. The etD gene was detected in 33 (39.75%) samples, but etC gene
no detected in all of isolates. In addition, probiotic lactobacilli species (spp.) e.g. L acidophillus, and
L reuteri against different isolates of methicillin resistence S aureus from different sites of infections
observed variance in their inhibitory activity ranging from 9-17mm. In addition, lactobacilli spp. were
collected from different sites and different results showed for S aureus in culture broth and cell free
culture supernatant. |
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| ISSN: | 0973-7510 2581-690X |