Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease
Prior studies reported inconsistent results on the altered gut microbial composition in patients with Crohn’s disease (CD), likely under the influences of many confounding factors including genetic, life style and environmental variations among different study cohorts. This study aims to examine the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.947313/full |
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| author | Jun Hu Jun Hu Jun Hu Sijing Cheng Sijing Cheng Sijing Cheng Sijing Cheng Jiayin Yao Jiayin Yao Jiayin Yao Xutao Lin Xutao Lin Xutao Lin Yichen Li Yichen Li Yichen Li Wenxia Wang Wenxia Wang Wenxia Wang Jingrong Weng Jingrong Weng Jingrong Weng Yifeng Zou Yifeng Zou Yifeng Zou Lixin Zhu Lixin Zhu Lixin Zhu Min Zhi Min Zhi Min Zhi |
| author_facet | Jun Hu Jun Hu Jun Hu Sijing Cheng Sijing Cheng Sijing Cheng Sijing Cheng Jiayin Yao Jiayin Yao Jiayin Yao Xutao Lin Xutao Lin Xutao Lin Yichen Li Yichen Li Yichen Li Wenxia Wang Wenxia Wang Wenxia Wang Jingrong Weng Jingrong Weng Jingrong Weng Yifeng Zou Yifeng Zou Yifeng Zou Lixin Zhu Lixin Zhu Lixin Zhu Min Zhi Min Zhi Min Zhi |
| author_sort | Jun Hu |
| collection | DOAJ |
| description | Prior studies reported inconsistent results on the altered gut microbial composition in patients with Crohn’s disease (CD), likely under the influences of many confounding factors including genetic, life style and environmental variations among different study cohorts. This study aims to examine the gut microbiota of CD patients with particular efforts to minimize the impact of the confounding factors. For this purpose, the healthy relatives of the patients were enrolled as control subjects so that the paired study subjects may have similar genetic background, dietary habits, and household environment. The fecal microbiota of the study subjects were examined by 16S rRNA sequencing. After the identification of the differential bacterial genera, multivariate regression analysis was performed to adjust the results for the impact of confounding factors. We found that the microbiota of the CD patients were featured with reduced short chain fatty acid (SCFA) producing bacteria and elevated opportunistic pathogen Escherichia-Shigella. Correlation analysis indicated that the elevation in Escherichia-Shigella and the reduction in SCFA-producing bacteria usually occur simultaneously. These differential genera exhibited a high capacity in distinguishing between CD and healthy controls achieving an area under curve of 0.89, and were correlated with the changes in inflammation related blood biochemical markers. Consistent with the reduction in SCFA-producing bacteria in CD, metabolomics analysis revealed decreased blood level of SCFAs in the patients. The differential genera identified in this study demonstrated outstanding capability to serve as diagnosis markers for CD and are potential targets for intervention. |
| first_indexed | 2024-12-10T19:46:26Z |
| format | Article |
| id | doaj.art-313899c5c80b494e83a808b3fa71186a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T19:46:26Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-313899c5c80b494e83a808b3fa71186a2022-12-22T01:35:52ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-08-011310.3389/fimmu.2022.947313947313Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s diseaseJun Hu0Jun Hu1Jun Hu2Sijing Cheng3Sijing Cheng4Sijing Cheng5Sijing Cheng6Jiayin Yao7Jiayin Yao8Jiayin Yao9Xutao Lin10Xutao Lin11Xutao Lin12Yichen Li13Yichen Li14Yichen Li15Wenxia Wang16Wenxia Wang17Wenxia Wang18Jingrong Weng19Jingrong Weng20Jingrong Weng21Yifeng Zou22Yifeng Zou23Yifeng Zou24Lixin Zhu25Lixin Zhu26Lixin Zhu27Min Zhi28Min Zhi29Min Zhi30Department of Gastroenterology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaSchool of Medicine, Sun Yat-sen University, Shenzhen, ChinaDepartment of Gastroenterology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastroenterology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaGuangdong Institute of Gastroenterology, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaPrior studies reported inconsistent results on the altered gut microbial composition in patients with Crohn’s disease (CD), likely under the influences of many confounding factors including genetic, life style and environmental variations among different study cohorts. This study aims to examine the gut microbiota of CD patients with particular efforts to minimize the impact of the confounding factors. For this purpose, the healthy relatives of the patients were enrolled as control subjects so that the paired study subjects may have similar genetic background, dietary habits, and household environment. The fecal microbiota of the study subjects were examined by 16S rRNA sequencing. After the identification of the differential bacterial genera, multivariate regression analysis was performed to adjust the results for the impact of confounding factors. We found that the microbiota of the CD patients were featured with reduced short chain fatty acid (SCFA) producing bacteria and elevated opportunistic pathogen Escherichia-Shigella. Correlation analysis indicated that the elevation in Escherichia-Shigella and the reduction in SCFA-producing bacteria usually occur simultaneously. These differential genera exhibited a high capacity in distinguishing between CD and healthy controls achieving an area under curve of 0.89, and were correlated with the changes in inflammation related blood biochemical markers. Consistent with the reduction in SCFA-producing bacteria in CD, metabolomics analysis revealed decreased blood level of SCFAs in the patients. The differential genera identified in this study demonstrated outstanding capability to serve as diagnosis markers for CD and are potential targets for intervention.https://www.frontiersin.org/articles/10.3389/fimmu.2022.947313/fullgut microbiomeinflammatory bowel diseaseshort chain fatty acidmultivariate regressionmetabolomics |
| spellingShingle | Jun Hu Jun Hu Jun Hu Sijing Cheng Sijing Cheng Sijing Cheng Sijing Cheng Jiayin Yao Jiayin Yao Jiayin Yao Xutao Lin Xutao Lin Xutao Lin Yichen Li Yichen Li Yichen Li Wenxia Wang Wenxia Wang Wenxia Wang Jingrong Weng Jingrong Weng Jingrong Weng Yifeng Zou Yifeng Zou Yifeng Zou Lixin Zhu Lixin Zhu Lixin Zhu Min Zhi Min Zhi Min Zhi Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease Frontiers in Immunology gut microbiome inflammatory bowel disease short chain fatty acid multivariate regression metabolomics |
| title | Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease |
| title_full | Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease |
| title_fullStr | Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease |
| title_full_unstemmed | Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease |
| title_short | Correlation between altered gut microbiota and elevated inflammation markers in patients with Crohn’s disease |
| title_sort | correlation between altered gut microbiota and elevated inflammation markers in patients with crohn s disease |
| topic | gut microbiome inflammatory bowel disease short chain fatty acid multivariate regression metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.947313/full |
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