Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae)
As part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH· (2,2-diphenyl-1-picrylhydrazyl-hydrate) and ABTS·+ [2,2'-azinobis(3-ethylenebenz...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/18/2/2376 |
| _version_ | 1818883324422127616 |
|---|---|
| author | Dulce Helena Siqueira Silva Vanderlan da Silva Bolzani Marcos Marçal Ferreira Queiroz Scheila Cristina Gutkoski Skrzek Luis Octávio Regasini Aline Coqueiro |
| author_facet | Dulce Helena Siqueira Silva Vanderlan da Silva Bolzani Marcos Marçal Ferreira Queiroz Scheila Cristina Gutkoski Skrzek Luis Octávio Regasini Aline Coqueiro |
| author_sort | Dulce Helena Siqueira Silva |
| collection | DOAJ |
| description | As part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH· (2,2-diphenyl-1-picrylhydrazyl-hydrate) and ABTS·+ [2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC50 of 3.5 ± 0.3 and 4.4 ± 0.2 μg mL−1 for DPPH· and 6.6 ± 0.4 and 3.1 ± 0.1 μg mL−1 for ABTS·+, respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O-b-glucoside (3), and quercetin-3-O-b-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported. |
| first_indexed | 2024-12-19T15:31:51Z |
| format | Article |
| id | doaj.art-313af8fc2e574925b7ead7c2060966f1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T15:31:51Z |
| publishDate | 2013-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-313af8fc2e574925b7ead7c2060966f12022-12-21T20:15:43ZengMDPI AGMolecules1420-30492013-02-011822376238510.3390/molecules18022376Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae)Dulce Helena Siqueira SilvaVanderlan da Silva BolzaniMarcos Marçal Ferreira QueirozScheila Cristina Gutkoski SkrzekLuis Octávio RegasiniAline CoqueiroAs part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH· (2,2-diphenyl-1-picrylhydrazyl-hydrate) and ABTS·+ [2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC50 of 3.5 ± 0.3 and 4.4 ± 0.2 μg mL−1 for DPPH· and 6.6 ± 0.4 and 3.1 ± 0.1 μg mL−1 for ABTS·+, respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O-b-glucoside (3), and quercetin-3-O-b-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported.http://www.mdpi.com/1420-3049/18/2/2376Kielmeyera variabilisClusiaceaeantioxidantfree radical scavenging activityDPPH·ABTS·+flavonoidflavonol |
| spellingShingle | Dulce Helena Siqueira Silva Vanderlan da Silva Bolzani Marcos Marçal Ferreira Queiroz Scheila Cristina Gutkoski Skrzek Luis Octávio Regasini Aline Coqueiro Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae) Molecules Kielmeyera variabilis Clusiaceae antioxidant free radical scavenging activity DPPH· ABTS·+ flavonoid flavonol |
| title | Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae) |
| title_full | Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae) |
| title_fullStr | Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae) |
| title_full_unstemmed | Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae) |
| title_short | Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae) |
| title_sort | free radical scavenging activity of kielmeyera variabilis clusiaceae |
| topic | Kielmeyera variabilis Clusiaceae antioxidant free radical scavenging activity DPPH· ABTS·+ flavonoid flavonol |
| url | http://www.mdpi.com/1420-3049/18/2/2376 |
| work_keys_str_mv | AT dulcehelenasiqueirasilva freeradicalscavengingactivityofkielmeyeravariabilisclusiaceae AT vanderlandasilvabolzani freeradicalscavengingactivityofkielmeyeravariabilisclusiaceae AT marcosmarcalferreiraqueiroz freeradicalscavengingactivityofkielmeyeravariabilisclusiaceae AT scheilacristinagutkoskiskrzek freeradicalscavengingactivityofkielmeyeravariabilisclusiaceae AT luisoctavioregasini freeradicalscavengingactivityofkielmeyeravariabilisclusiaceae AT alinecoqueiro freeradicalscavengingactivityofkielmeyeravariabilisclusiaceae |