In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i>
Context: <i>Salvia balansae</i> de Noé (<i>S. balansae</i>) (Lamiaceae) is known to be an important plant used in folk medicine as an herbal remedy in Algeria. Objective: The purpose of the present study was to demonstrate the phytochemical composition, antioxidant activities...
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MDPI AG
2023-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/23/7801 |
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| author | Amırat Mokhtar Tabak Souhila Bouriah Nacéra Benabdallah Amina Mohammed I. Alghonaim Mehmet Öztürk Sulaiman A. Alsalamah Mohamed Djamel Miara Fehmi Boufahja Hamdi Bendif |
| author_facet | Amırat Mokhtar Tabak Souhila Bouriah Nacéra Benabdallah Amina Mohammed I. Alghonaim Mehmet Öztürk Sulaiman A. Alsalamah Mohamed Djamel Miara Fehmi Boufahja Hamdi Bendif |
| author_sort | Amırat Mokhtar |
| collection | DOAJ |
| description | Context: <i>Salvia balansae</i> de Noé (<i>S. balansae</i>) (Lamiaceae) is known to be an important plant used in folk medicine as an herbal remedy in Algeria. Objective: The purpose of the present study was to demonstrate the phytochemical composition, antioxidant activities, enzyme inhibitory activities, and antimicrobial activities of <i>S. balansae</i> extracts. Materials and methods: A methanolic extract and a petroleum ether extract from the aerial parts of the plant were assessed for their chemical composition. HPLC-MS and HPLC-DAD assessed the content of phenols, GC-MS the fatty acid composition, and ICP-MS the mineral profiles of the plant. Additionally, we evaluated the bioactivities of <i>S. balansae</i> extracts by the DPPH, ABTS, and CUPRAC assays, including the antioxidant potential against AChE, BChE, α-amylase, and α-glucosidase for enzyme inhibition. The antibacterial and antifungal activities of the methanolic extract were determined by the disc diffusion test against several strains of bacteria and yeasts. Results: Our findings revealed that the aerial parts of <i>S. balansae</i> were rich in phytochemical components and contained large amounts of minerals. Quantitative analysis of phenolic compounds by HPLC-DAD revealed the presence of 12 compounds in three major classes, flavonoids, hydroxycinnamic acid, and phenolic acid derivatives, with 0.61, 0.45, and 0.29 mg/g of extract, respectively. Nine phenolic constituents were quantified by HPLC-MS analysis; catechin (72.5%) was the main compound, followed by myricetin (21.7%). The fatty acid composition of the <i>S. balansae</i> petroleum ether extract by GC-MS analysis was quantified. Seventeen compounds, including palmitic acid, were identified as the major fatty acids. The antioxidant activity of the <i>S. balansae</i> extracts was measured by three different methods: the methanol extract provided better results than the petroleum ether extract, and interesting values were noted for the DPPH, ABTS, and CUPRAC assays of 242.7 ± 7.44, 124.1 ± 9.70, and 222.9 ± 6.05 µg/mL, respectively. The enzyme inhibition activity of the plant could not be determined. The antimicrobial results of the methanolic extract obtained from the disc diffusion method, followed by measurements of MIC, MBC, and MFC against several bacteria and yeasts, indicated that <i>S. balansae</i> exhibited noticeable antimicrobial and antifungal activities. Conclusions: These results provided new data about the main phenolic compounds and biological activities of extracts of the aerial parts of <i>S. balansae</i>, which might be an alternative source for synthetic bioactive compounds. |
| first_indexed | 2024-03-09T01:45:45Z |
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| language | English |
| last_indexed | 2024-03-09T01:45:45Z |
| publishDate | 2023-11-01 |
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| series | Molecules |
| spelling | doaj.art-313bdaa608f146aa901ca092a041f80b2023-12-08T15:22:23ZengMDPI AGMolecules1420-30492023-11-012823780110.3390/molecules28237801In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i>Amırat Mokhtar0Tabak Souhila1Bouriah Nacéra2Benabdallah Amina3Mohammed I. Alghonaim4Mehmet Öztürk5Sulaiman A. Alsalamah6Mohamed Djamel Miara7Fehmi Boufahja8Hamdi Bendif9Institute of Veterinary Sciences, University Ibn-Khaldoun of Tiaret, Tiaret 14000, AlgeriaDepartment of Nature and Life Sciences, Faculty of Life and Nature Sciences, University of Tiaret, Tiaret 14000, AlgeriaDepartment of Nature and Life Sciences, Faculty of Life and Nature Sciences, University of Tiaret, Tiaret 14000, AlgeriaDepartment of Agronomy, Faculty of Life and Nature Sciences, University Chadli Bendjedid, El-Tarf 36000, AlgeriaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaDepartment of Chemistry, Faculty of Sciences, Muğla Sıtkı Koçman University, Muğla 48121, TürkiyeBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaDepartment of Nature and Life Sciences, Faculty of Life and Nature Sciences, University of Tiaret, Tiaret 14000, AlgeriaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaLaboratory of Ethnobotany and Natural Substances, ENS de Kouba, Algiers 16308, AlgeriaContext: <i>Salvia balansae</i> de Noé (<i>S. balansae</i>) (Lamiaceae) is known to be an important plant used in folk medicine as an herbal remedy in Algeria. Objective: The purpose of the present study was to demonstrate the phytochemical composition, antioxidant activities, enzyme inhibitory activities, and antimicrobial activities of <i>S. balansae</i> extracts. Materials and methods: A methanolic extract and a petroleum ether extract from the aerial parts of the plant were assessed for their chemical composition. HPLC-MS and HPLC-DAD assessed the content of phenols, GC-MS the fatty acid composition, and ICP-MS the mineral profiles of the plant. Additionally, we evaluated the bioactivities of <i>S. balansae</i> extracts by the DPPH, ABTS, and CUPRAC assays, including the antioxidant potential against AChE, BChE, α-amylase, and α-glucosidase for enzyme inhibition. The antibacterial and antifungal activities of the methanolic extract were determined by the disc diffusion test against several strains of bacteria and yeasts. Results: Our findings revealed that the aerial parts of <i>S. balansae</i> were rich in phytochemical components and contained large amounts of minerals. Quantitative analysis of phenolic compounds by HPLC-DAD revealed the presence of 12 compounds in three major classes, flavonoids, hydroxycinnamic acid, and phenolic acid derivatives, with 0.61, 0.45, and 0.29 mg/g of extract, respectively. Nine phenolic constituents were quantified by HPLC-MS analysis; catechin (72.5%) was the main compound, followed by myricetin (21.7%). The fatty acid composition of the <i>S. balansae</i> petroleum ether extract by GC-MS analysis was quantified. Seventeen compounds, including palmitic acid, were identified as the major fatty acids. The antioxidant activity of the <i>S. balansae</i> extracts was measured by three different methods: the methanol extract provided better results than the petroleum ether extract, and interesting values were noted for the DPPH, ABTS, and CUPRAC assays of 242.7 ± 7.44, 124.1 ± 9.70, and 222.9 ± 6.05 µg/mL, respectively. The enzyme inhibition activity of the plant could not be determined. The antimicrobial results of the methanolic extract obtained from the disc diffusion method, followed by measurements of MIC, MBC, and MFC against several bacteria and yeasts, indicated that <i>S. balansae</i> exhibited noticeable antimicrobial and antifungal activities. Conclusions: These results provided new data about the main phenolic compounds and biological activities of extracts of the aerial parts of <i>S. balansae</i>, which might be an alternative source for synthetic bioactive compounds.https://www.mdpi.com/1420-3049/28/23/7801antibacterialantioxidantanticholinesteraseantidiabeticchemical composition<i>Salvia balansae</i> |
| spellingShingle | Amırat Mokhtar Tabak Souhila Bouriah Nacéra Benabdallah Amina Mohammed I. Alghonaim Mehmet Öztürk Sulaiman A. Alsalamah Mohamed Djamel Miara Fehmi Boufahja Hamdi Bendif In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i> Molecules antibacterial antioxidant anticholinesterase antidiabetic chemical composition <i>Salvia balansae</i> |
| title | In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i> |
| title_full | In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i> |
| title_fullStr | In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i> |
| title_full_unstemmed | In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i> |
| title_short | In Vitro Antibacterial, Antioxidant, Anticholinesterase, and Antidiabetic Activities and Chemical Composition of <i>Salvia balansae</i> |
| title_sort | in vitro antibacterial antioxidant anticholinesterase and antidiabetic activities and chemical composition of i salvia balansae i |
| topic | antibacterial antioxidant anticholinesterase antidiabetic chemical composition <i>Salvia balansae</i> |
| url | https://www.mdpi.com/1420-3049/28/23/7801 |
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