Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease
Abstract 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immunosorbent assay in DNA from post-mortem c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-02-01
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| Series: | npj Parkinson's Disease |
| Online Access: | https://doi.org/10.1038/s41531-017-0007-3 |
| _version_ | 1827614932017872896 |
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| author | Reinhard Stöger Paula J. Scaife Freya Shephard Lisa Chakrabarti |
| author_facet | Reinhard Stöger Paula J. Scaife Freya Shephard Lisa Chakrabarti |
| author_sort | Reinhard Stöger |
| collection | DOAJ |
| description | Abstract 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immunosorbent assay in DNA from post-mortem cerebella of individuals with Parkinson’s disease and age-matched controls. 5-methylcytosine levels showed no significant differences between Parkinson’s disease and control DNA sample sets. In contrast, median 5-hydroxymethylcytosine levels were almost twice as high (p < 0.001) in both male and female Parkinson’s disease individuals compared with controls. The distinct epigenetic profile identified in cerebellar DNA of Parkinson’s disease patients raises the question whether elevated 5-hydroxymethylcytosine levels are a driver or a consequence of Parkinson’s disease. |
| first_indexed | 2024-03-09T09:01:56Z |
| format | Article |
| id | doaj.art-313bead6c40041298b4aafaba6c38a62 |
| institution | Directory Open Access Journal |
| issn | 2373-8057 |
| language | English |
| last_indexed | 2024-03-09T09:01:56Z |
| publishDate | 2017-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Parkinson's Disease |
| spelling | doaj.art-313bead6c40041298b4aafaba6c38a622023-12-02T11:14:03ZengNature Portfolionpj Parkinson's Disease2373-80572017-02-01311310.1038/s41531-017-0007-3Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s diseaseReinhard Stöger0Paula J. Scaife1Freya Shephard2Lisa Chakrabarti3School of Biosciences, University of NottinghamSchool of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of NottinghamSchool of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of NottinghamSchool of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of NottinghamAbstract 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immunosorbent assay in DNA from post-mortem cerebella of individuals with Parkinson’s disease and age-matched controls. 5-methylcytosine levels showed no significant differences between Parkinson’s disease and control DNA sample sets. In contrast, median 5-hydroxymethylcytosine levels were almost twice as high (p < 0.001) in both male and female Parkinson’s disease individuals compared with controls. The distinct epigenetic profile identified in cerebellar DNA of Parkinson’s disease patients raises the question whether elevated 5-hydroxymethylcytosine levels are a driver or a consequence of Parkinson’s disease.https://doi.org/10.1038/s41531-017-0007-3 |
| spellingShingle | Reinhard Stöger Paula J. Scaife Freya Shephard Lisa Chakrabarti Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease npj Parkinson's Disease |
| title | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_full | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_fullStr | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_full_unstemmed | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_short | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_sort | elevated 5hmc levels characterize dna of the cerebellum in parkinson s disease |
| url | https://doi.org/10.1038/s41531-017-0007-3 |
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