DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY
The aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar). For th...
| Main Authors: | , |
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| Format: | Article |
| Language: | Russian |
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Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute
2016-03-01
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| Series: | Фармация и фармакология (Пятигорск) |
| Subjects: | |
| Online Access: | https://www.pharmpharm.ru/jour/article/view/191 |
| _version_ | 1797874741200027648 |
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| author | E. G. Sannikova T. D. Mezenova |
| author_facet | E. G. Sannikova T. D. Mezenova |
| author_sort | E. G. Sannikova |
| collection | DOAJ |
| description | The aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar). For the chromatography analysis we used plates «Sorbfil» PTSH-P-A-UV, mobile phase: n-butanol-glacial acetic acid-water (4:1:1), detecting reagent – ammonia vapors. The rutin is detected as yellow spots with Rf 0.64±0.02. The detection limit is 0.5 μg/μl. The regression equation has the form: S=4.75×103 m. With the use of this method we found that the composition of rutin in leaves of Salix triandra was in the range 1.81±0.06% on the air-dry raw material (relative standard deviation RSD%=2.5%; the measurement error E=3.12%). The method is sensitive, linear, has a small error, and does not require expensive equipment. |
| first_indexed | 2024-04-10T01:35:41Z |
| format | Article |
| id | doaj.art-313fc2716f1144f08d50efed8b0d3fd5 |
| institution | Directory Open Access Journal |
| issn | 2307-9266 2413-2241 |
| language | Russian |
| last_indexed | 2024-04-10T01:35:41Z |
| publishDate | 2016-03-01 |
| publisher | Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute |
| record_format | Article |
| series | Фармация и фармакология (Пятигорск) |
| spelling | doaj.art-313fc2716f1144f08d50efed8b0d3fd52023-03-13T09:18:11ZrusVolgograd State Medical University, Pyatigorsk Medical and Pharmaceutical InstituteФармация и фармакология (Пятигорск)2307-92662413-22412016-03-0141(14)859010.19163/2307-9266-2016-4-1(14)-85-90190DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHYE. G. Sannikova0T. D. Mezenova1Пятигорский медико-фармацевтический институт – филиал ГБОУ ВПО ВолгГМУ Минздрава России, г. ПятигорскПятигорский медико-фармацевтический институт – филиал ГБОУ ВПО ВолгГМУ Минздрава России, г. ПятигорскThe aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar). For the chromatography analysis we used plates «Sorbfil» PTSH-P-A-UV, mobile phase: n-butanol-glacial acetic acid-water (4:1:1), detecting reagent – ammonia vapors. The rutin is detected as yellow spots with Rf 0.64±0.02. The detection limit is 0.5 μg/μl. The regression equation has the form: S=4.75×103 m. With the use of this method we found that the composition of rutin in leaves of Salix triandra was in the range 1.81±0.06% on the air-dry raw material (relative standard deviation RSD%=2.5%; the measurement error E=3.12%). The method is sensitive, linear, has a small error, and does not require expensive equipment.https://www.pharmpharm.ru/jour/article/view/191рутинлистья ивы трёхтычинковой (salix triandra l.)тонкослойная хроматографияидентификацияколичественное определение«видеоденситометр sorbfil». |
| spellingShingle | E. G. Sannikova T. D. Mezenova DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY Фармация и фармакология (Пятигорск) рутин листья ивы трёхтычинковой (salix triandra l.) тонкослойная хроматография идентификация количественное определение «видеоденситометр sorbfil». |
| title | DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY |
| title_full | DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY |
| title_fullStr | DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY |
| title_full_unstemmed | DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY |
| title_short | DETERMINATION OF RUTIN IN LEAVES OF SALIX TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY |
| title_sort | determination of rutin in leaves of salix triandra using the method of planar chromatography |
| topic | рутин листья ивы трёхтычинковой (salix triandra l.) тонкослойная хроматография идентификация количественное определение «видеоденситометр sorbfil». |
| url | https://www.pharmpharm.ru/jour/article/view/191 |
| work_keys_str_mv | AT egsannikova determinationofrutininleavesofsalixtriandrausingthemethodofplanarchromatography AT tdmezenova determinationofrutininleavesofsalixtriandrausingthemethodofplanarchromatography |