Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets
Meconium aspiration syndrome causes respiratory failure after birth and in vivo monitoring of pulmonary edema is difficult. The objective of the present study was to assess hemodynamic changes and edema measured by transcardiopulmonary thermodilution in low weight newborn piglets. Additionally, the...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2011-08-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800007 |
| _version_ | 1819000591725101056 |
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| author | F.E. Silvera M.F. Blasina L. Vaamonde S. Tellechea C. Godoy S. Zabala G. Mañana M. Martell W. Olivera |
| author_facet | F.E. Silvera M.F. Blasina L. Vaamonde S. Tellechea C. Godoy S. Zabala G. Mañana M. Martell W. Olivera |
| author_sort | F.E. Silvera |
| collection | DOAJ |
| description | Meconium aspiration syndrome causes respiratory failure after birth and in vivo monitoring of pulmonary edema is difficult. The objective of the present study was to assess hemodynamic changes and edema measured by transcardiopulmonary thermodilution in low weight newborn piglets. Additionally, the effect of early administration of sildenafil (2 mg/kg vo, 30 min after meconium aspiration) on this critical parameter was determined in the meconium aspiration syndrome model. Thirty-eight mechanically ventilated anesthetized male piglets (Sus scrofa domestica) aged 12 to 72 h (1660 ± 192 g) received diluted fresh human meconium in the airway in order to evoke pulmonary hypertension (PHT). Extravascular lung water was measured in vivo with a PiCCO monitor and ex vivo by the gravimetric method, resulting in an overestimate of 3.5 ± 2.3 mL compared to the first measurement. A significant PHT of 15 Torr above basal pressure was observed, similar to that of severely affected humans, leading to an increase in ventilatory support. The vascular permeability index increased 57%, suggesting altered alveolocapillary membrane permeability. Histology revealed tissue vessel congestion and nonspecific chemical pneumonitis. A group of animals received sildenafil, which prevented the development of PHT and lung edema, as evaluated by in vivo monitoring. In summary, the transcardiopulmonary thermodilution method is a reliable tool for monitoring critical newborn changes, offering the opportunity to experimentally explore putative therapeutics in vivo. Sildenafil could be employed to prevent PHT and edema if used in the first stages of development of the disease. |
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| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-20T22:35:45Z |
| publishDate | 2011-08-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-3141161e9f52453aa41874135bd1cdd22022-12-21T19:24:37ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2011-08-01448778785Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn pigletsF.E. SilveraM.F. BlasinaL. VaamondeS. TellecheaC. GodoyS. ZabalaG. MañanaM. MartellW. OliveraMeconium aspiration syndrome causes respiratory failure after birth and in vivo monitoring of pulmonary edema is difficult. The objective of the present study was to assess hemodynamic changes and edema measured by transcardiopulmonary thermodilution in low weight newborn piglets. Additionally, the effect of early administration of sildenafil (2 mg/kg vo, 30 min after meconium aspiration) on this critical parameter was determined in the meconium aspiration syndrome model. Thirty-eight mechanically ventilated anesthetized male piglets (Sus scrofa domestica) aged 12 to 72 h (1660 ± 192 g) received diluted fresh human meconium in the airway in order to evoke pulmonary hypertension (PHT). Extravascular lung water was measured in vivo with a PiCCO monitor and ex vivo by the gravimetric method, resulting in an overestimate of 3.5 ± 2.3 mL compared to the first measurement. A significant PHT of 15 Torr above basal pressure was observed, similar to that of severely affected humans, leading to an increase in ventilatory support. The vascular permeability index increased 57%, suggesting altered alveolocapillary membrane permeability. Histology revealed tissue vessel congestion and nonspecific chemical pneumonitis. A group of animals received sildenafil, which prevented the development of PHT and lung edema, as evaluated by in vivo monitoring. In summary, the transcardiopulmonary thermodilution method is a reliable tool for monitoring critical newborn changes, offering the opportunity to experimentally explore putative therapeutics in vivo. Sildenafil could be employed to prevent PHT and edema if used in the first stages of development of the disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800007Lung waterMeconium aspiration syndromeNewbornSildenafilPulmonary hypertension |
| spellingShingle | F.E. Silvera M.F. Blasina L. Vaamonde S. Tellechea C. Godoy S. Zabala G. Mañana M. Martell W. Olivera Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets Brazilian Journal of Medical and Biological Research Lung water Meconium aspiration syndrome Newborn Sildenafil Pulmonary hypertension |
| title | Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets |
| title_full | Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets |
| title_fullStr | Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets |
| title_full_unstemmed | Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets |
| title_short | Sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets |
| title_sort | sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets |
| topic | Lung water Meconium aspiration syndrome Newborn Sildenafil Pulmonary hypertension |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800007 |
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