OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions
Context (Background): Stem cells are a unique subpopulation of cells in the human body with a capacity to initiate differentiation into various cell lines. Tumor stem cells (TSCs) are a unique subpopulation of cells that possess the ability to initiate a neoplasm and sustain self-renewal. Epithelial...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Journal of Orofacial Sciences |
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| Online Access: | http://www.jofs.in/article.asp?issn=0975-8844;year=2016;volume=8;issue=1;spage=16;epage=21;aulast=Banerjee |
| _version_ | 1818254353980456960 |
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| author | Abhishek Banerjee Venkatesh Vishwanath Kamath Lavanya Sundaram Shruthi S Krishnamurthy |
| author_facet | Abhishek Banerjee Venkatesh Vishwanath Kamath Lavanya Sundaram Shruthi S Krishnamurthy |
| author_sort | Abhishek Banerjee |
| collection | DOAJ |
| description | Context (Background): Stem cells are a unique subpopulation of cells in the human body with a capacity to initiate differentiation into various cell lines. Tumor stem cells (TSCs) are a unique subpopulation of cells that possess the ability to initiate a neoplasm and sustain self-renewal. Epithelial stem cell (ESC) markers such as octamer-binding transcription factor 4 (OCT4) and sex-determining region Y (SRY)-box 2 (SOX2) are capable of identifying these stem cells expressed during the early stages of tooth development. Aims: To detect the expression of the stem cell markers OCT4 and SOX2 in the normal odontogenic tissues and the odontogenic cysts and tumors. Materials and Methods: Paraffin sections of follicular tissue, radicular cyst, dentigerous cyst, odontogenic keratocyst, ameloblastoma, adenomatoid odontogenic tumor, and ameloblastic carcinoma were obtained from the archives. The sections were subjected to immunohistochemical assay by the use of mouse monoclonal antibodies to OCT4 and SOX2. Statistical Analysis: The results were evaluated by descriptive analysis. Results: The results show the presence of stem cells in the normal and lesional tissues with these stem cell identifying markers. SOX2 was found to be more consistent and reliable in the detection of stem cells. Conclusion: The stem cell expressions are maintained in the tumor transformation of tissue and probably suggest that there is no phenotypic change of stem cells in progression from normal embryonic state to its tumor component. The quantification and localization reveals interesting trends that indicate the probable role of the cells in the pathogenesis of the lesions. |
| first_indexed | 2024-12-12T16:54:38Z |
| format | Article |
| id | doaj.art-3141cbdfb73c4ef2bfa100674c633ee7 |
| institution | Directory Open Access Journal |
| issn | 0975-8844 |
| language | English |
| last_indexed | 2024-12-12T16:54:38Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Orofacial Sciences |
| spelling | doaj.art-3141cbdfb73c4ef2bfa100674c633ee72022-12-22T00:18:15ZengWolters Kluwer Medknow PublicationsJournal of Orofacial Sciences0975-88442016-01-0181162110.4103/0975-8844.181920OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesionsAbhishek BanerjeeVenkatesh Vishwanath KamathLavanya SundaramShruthi S KrishnamurthyContext (Background): Stem cells are a unique subpopulation of cells in the human body with a capacity to initiate differentiation into various cell lines. Tumor stem cells (TSCs) are a unique subpopulation of cells that possess the ability to initiate a neoplasm and sustain self-renewal. Epithelial stem cell (ESC) markers such as octamer-binding transcription factor 4 (OCT4) and sex-determining region Y (SRY)-box 2 (SOX2) are capable of identifying these stem cells expressed during the early stages of tooth development. Aims: To detect the expression of the stem cell markers OCT4 and SOX2 in the normal odontogenic tissues and the odontogenic cysts and tumors. Materials and Methods: Paraffin sections of follicular tissue, radicular cyst, dentigerous cyst, odontogenic keratocyst, ameloblastoma, adenomatoid odontogenic tumor, and ameloblastic carcinoma were obtained from the archives. The sections were subjected to immunohistochemical assay by the use of mouse monoclonal antibodies to OCT4 and SOX2. Statistical Analysis: The results were evaluated by descriptive analysis. Results: The results show the presence of stem cells in the normal and lesional tissues with these stem cell identifying markers. SOX2 was found to be more consistent and reliable in the detection of stem cells. Conclusion: The stem cell expressions are maintained in the tumor transformation of tissue and probably suggest that there is no phenotypic change of stem cells in progression from normal embryonic state to its tumor component. The quantification and localization reveals interesting trends that indicate the probable role of the cells in the pathogenesis of the lesions.http://www.jofs.in/article.asp?issn=0975-8844;year=2016;volume=8;issue=1;spage=16;epage=21;aulast=BanerjeeOctamer-binding transcription factor 4 (OCT4), odontogenic lesions, sex-determining region Y (SRY)-box 2 (SOX2), stem cells |
| spellingShingle | Abhishek Banerjee Venkatesh Vishwanath Kamath Lavanya Sundaram Shruthi S Krishnamurthy OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions Journal of Orofacial Sciences Octamer-binding transcription factor 4 (OCT4), odontogenic lesions, sex-determining region Y (SRY)-box 2 (SOX2), stem cells |
| title | OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions |
| title_full | OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions |
| title_fullStr | OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions |
| title_full_unstemmed | OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions |
| title_short | OCT4 and SOX2 are reliable markers in detecting stem cells in odontogenic lesions |
| title_sort | oct4 and sox2 are reliable markers in detecting stem cells in odontogenic lesions |
| topic | Octamer-binding transcription factor 4 (OCT4), odontogenic lesions, sex-determining region Y (SRY)-box 2 (SOX2), stem cells |
| url | http://www.jofs.in/article.asp?issn=0975-8844;year=2016;volume=8;issue=1;spage=16;epage=21;aulast=Banerjee |
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