Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein.
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine effi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5770062?pdf=render |
| _version_ | 1818945304858198016 |
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| author | Ah-Ra Kim Dong-Hun Lee Su-Hwa Lee Ilaria Rubino Hyo-Jick Choi Fu-Shi Quan |
| author_facet | Ah-Ra Kim Dong-Hun Lee Su-Hwa Lee Ilaria Rubino Hyo-Jick Choi Fu-Shi Quan |
| author_sort | Ah-Ra Kim |
| collection | DOAJ |
| description | Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine efficacy in a mouse model. For VLP vaccines, tandem gene (1-780 bp) for V1 VLPs and tandem repeat gene (repeated 450-780 bp) for V5 VLPs were constructed in pFastBacTM vectors, respectively. Influenza matrix protein 1 (M1) was used as a core protein in the VLPs. Notably, upon challenge infection, significantly lower virus loads were measured in the lung of mice immunized with V1 or V5 VLPs compared to those of naïve mice and formalin-inactivated RSV immunized control mice. In particular, V5 VLPs immunization showed significantly lower virus titers than V1 VLPs immunization. Furthermore, V5 VLPs immunization elicited increased memory B cells responses in the spleen. These results indicated that V5 VLP vaccine containing tandem repeat gene protein provided better protection than V1 VLPs with significantly decreased inflammation in the lungs. Thus, V5 VLPs could be a potential vaccine candidate against RSV. |
| first_indexed | 2024-12-20T07:57:00Z |
| format | Article |
| id | doaj.art-3141d2170857443eb36d896078790879 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T07:57:00Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3141d2170857443eb36d8960787908792022-12-21T19:47:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019127710.1371/journal.pone.0191277Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein.Ah-Ra KimDong-Hun LeeSu-Hwa LeeIlaria RubinoHyo-Jick ChoiFu-Shi QuanRespiratory syncytial virus (RSV) is the leading cause of lower respiratory tract illness in infants, young children and the elderly. However, there is no licensed vaccine available against RSV infection. In this study, we generated virus-like particle (VLP) vaccine and investigated the vaccine efficacy in a mouse model. For VLP vaccines, tandem gene (1-780 bp) for V1 VLPs and tandem repeat gene (repeated 450-780 bp) for V5 VLPs were constructed in pFastBacTM vectors, respectively. Influenza matrix protein 1 (M1) was used as a core protein in the VLPs. Notably, upon challenge infection, significantly lower virus loads were measured in the lung of mice immunized with V1 or V5 VLPs compared to those of naïve mice and formalin-inactivated RSV immunized control mice. In particular, V5 VLPs immunization showed significantly lower virus titers than V1 VLPs immunization. Furthermore, V5 VLPs immunization elicited increased memory B cells responses in the spleen. These results indicated that V5 VLP vaccine containing tandem repeat gene protein provided better protection than V1 VLPs with significantly decreased inflammation in the lungs. Thus, V5 VLPs could be a potential vaccine candidate against RSV.http://europepmc.org/articles/PMC5770062?pdf=render |
| spellingShingle | Ah-Ra Kim Dong-Hun Lee Su-Hwa Lee Ilaria Rubino Hyo-Jick Choi Fu-Shi Quan Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein. PLoS ONE |
| title | Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein. |
| title_full | Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein. |
| title_fullStr | Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein. |
| title_full_unstemmed | Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein. |
| title_short | Protection induced by virus-like particle vaccine containing tandem repeat gene of respiratory syncytial virus G protein. |
| title_sort | protection induced by virus like particle vaccine containing tandem repeat gene of respiratory syncytial virus g protein |
| url | http://europepmc.org/articles/PMC5770062?pdf=render |
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