Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro
Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/11/1721 |
| _version_ | 1797511060787298304 |
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| author | Marta De Angelis David Della-Morte Gabriele Buttinelli Angela Di Martino Francesca Pacifici Paola Checconi Luigina Ambrosio Paola Stefanelli Anna Teresa Palamara Enrico Garaci Camillo Ricordi Lucia Nencioni |
| author_facet | Marta De Angelis David Della-Morte Gabriele Buttinelli Angela Di Martino Francesca Pacifici Paola Checconi Luigina Ambrosio Paola Stefanelli Anna Teresa Palamara Enrico Garaci Camillo Ricordi Lucia Nencioni |
| author_sort | Marta De Angelis |
| collection | DOAJ |
| description | Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the present work, we evaluated the antiviral activity of polydatin, an RV precursor, and a mixture of polyphenols and other micronutrients, named A5+, against influenza virus and SARS-CoV-2 infections. To this end, we infected Vero E6 cells and analyzed the replication of both respiratory viruses in terms of viral proteins synthesis and viral titration. We demonstrated that A5+ showed a higher efficacy in inhibiting both influenza virus and SARS-CoV-2 infections compared to polydatin treatment alone. Indeed, post infection treatment significantly decreased viral proteins expression and viral release, probably by interfering with any step of virus replicative cycle. Intriguingly, A5+ treatment strongly reduced IL-6 cytokine production in influenza virus-infected cells, suggesting its potential anti-inflammatory properties during the infection. Overall, these results demonstrate the synergic and innovative antiviral efficacy of A5+ mixture, although further studies are needed to clarify the mechanisms underlying its inhibitory effect. |
| first_indexed | 2024-03-10T05:41:00Z |
| format | Article |
| id | doaj.art-3144b63ceb8b4be4adc02d4e6d741ae1 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:00Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-3144b63ceb8b4be4adc02d4e6d741ae12023-11-22T22:32:27ZengMDPI AGBiomedicines2227-90592021-11-01911172110.3390/biomedicines9111721Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In VitroMarta De Angelis0David Della-Morte1Gabriele Buttinelli2Angela Di Martino3Francesca Pacifici4Paola Checconi5Luigina Ambrosio6Paola Stefanelli7Anna Teresa Palamara8Enrico Garaci9Camillo Ricordi10Lucia Nencioni11Laboratory Affiliated to Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Systems Medicine, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Human Sciences and Quality of Life Promotion, San Raffaele Roma Open University, IRCCS San Raffaele Roma, 00166 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyLaboratory Affiliated to Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Human Sciences and Quality of Life Promotion, San Raffaele Roma Open University, IRCCS San Raffaele Roma, 00166 Rome, ItalyCell Transplant Center, Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USALaboratory Affiliated to Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, ItalyPolyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has been demonstrated to inhibit influenza virus replication and more recently, it has been tested together with pterostilbene against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the present work, we evaluated the antiviral activity of polydatin, an RV precursor, and a mixture of polyphenols and other micronutrients, named A5+, against influenza virus and SARS-CoV-2 infections. To this end, we infected Vero E6 cells and analyzed the replication of both respiratory viruses in terms of viral proteins synthesis and viral titration. We demonstrated that A5+ showed a higher efficacy in inhibiting both influenza virus and SARS-CoV-2 infections compared to polydatin treatment alone. Indeed, post infection treatment significantly decreased viral proteins expression and viral release, probably by interfering with any step of virus replicative cycle. Intriguingly, A5+ treatment strongly reduced IL-6 cytokine production in influenza virus-infected cells, suggesting its potential anti-inflammatory properties during the infection. Overall, these results demonstrate the synergic and innovative antiviral efficacy of A5+ mixture, although further studies are needed to clarify the mechanisms underlying its inhibitory effect.https://www.mdpi.com/2227-9059/9/11/1721SARS-CoV-2influenza virusantiviralspolyphenolsmicronutrientsCOVID-19 |
| spellingShingle | Marta De Angelis David Della-Morte Gabriele Buttinelli Angela Di Martino Francesca Pacifici Paola Checconi Luigina Ambrosio Paola Stefanelli Anna Teresa Palamara Enrico Garaci Camillo Ricordi Lucia Nencioni Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro Biomedicines SARS-CoV-2 influenza virus antivirals polyphenols micronutrients COVID-19 |
| title | Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro |
| title_full | Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro |
| title_fullStr | Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro |
| title_full_unstemmed | Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro |
| title_short | Protective Role of Combined Polyphenols and Micronutrients against Influenza A Virus and SARS-CoV-2 Infection In Vitro |
| title_sort | protective role of combined polyphenols and micronutrients against influenza a virus and sars cov 2 infection in vitro |
| topic | SARS-CoV-2 influenza virus antivirals polyphenols micronutrients COVID-19 |
| url | https://www.mdpi.com/2227-9059/9/11/1721 |
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