Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i>
<i>Verticillium dahliae</i>, a virulent soil-borne fungus, elicits Verticillium wilt in numerous dicotyledonous plants through intricate pathogenic mechanisms. Ubiquitination, an evolutionarily conserved post-translational modification, marks and labels proteins for degradation, thereby...
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MDPI AG
2023-10-01
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| Series: | Journal of Fungi |
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| author | Xing Yang Zhijuan Hu Jingjie Yuan Run Zou Yilan Wang Xuan Peng Shan Xu Chengjian Xie |
| author_facet | Xing Yang Zhijuan Hu Jingjie Yuan Run Zou Yilan Wang Xuan Peng Shan Xu Chengjian Xie |
| author_sort | Xing Yang |
| collection | DOAJ |
| description | <i>Verticillium dahliae</i>, a virulent soil-borne fungus, elicits Verticillium wilt in numerous dicotyledonous plants through intricate pathogenic mechanisms. Ubiquitination, an evolutionarily conserved post-translational modification, marks and labels proteins for degradation, thereby maintaining cellular homeostasis. Within the ubiquitination cascade, ubiquitin ligase E3 demonstrates a unique capability for target protein recognition, a function often implicated in phytopathogenic virulence. Our research indicates that two ubiquitin ligase E3s, VdBre1 and VdHrd1, are intrinsically associated with virulence. Our findings demonstrate that the deletion of these two genes significantly impairs the ability of <i>V. dahliae</i> to colonize the vascular bundles of plants and to form typical penetration pegs. Furthermore, transcriptomic analysis suggests that VdBre1 governs the lipid metabolism pathway, while VdHrd1 participates in endoplasmic-reticulum-related processes. Western blot analyses reveal a significant decrease in histone ubiquitination and histone H3K4 trimethylation levels in the <i>ΔVdBre1</i> mutant. This research illuminates the function of ubiquitin ligase E3 in <i>V. dahliae</i> and offers fresh theoretical perspectives. Our research identifies two novel virulence-related genes and partially explicates their roles in virulence-associated structures and gene regulatory pathways. These findings augment our understanding of the molecular mechanisms inherent to <i>V. dahliae</i>. |
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| spelling | doaj.art-314539a251d84d86887d776a84edb2762023-11-19T17:01:22ZengMDPI AGJournal of Fungi2309-608X2023-10-01910103710.3390/jof9101037Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i>Xing Yang0Zhijuan Hu1Jingjie Yuan2Run Zou3Yilan Wang4Xuan Peng5Shan Xu6Chengjian Xie7The Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, ChinaThe Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, China<i>Verticillium dahliae</i>, a virulent soil-borne fungus, elicits Verticillium wilt in numerous dicotyledonous plants through intricate pathogenic mechanisms. Ubiquitination, an evolutionarily conserved post-translational modification, marks and labels proteins for degradation, thereby maintaining cellular homeostasis. Within the ubiquitination cascade, ubiquitin ligase E3 demonstrates a unique capability for target protein recognition, a function often implicated in phytopathogenic virulence. Our research indicates that two ubiquitin ligase E3s, VdBre1 and VdHrd1, are intrinsically associated with virulence. Our findings demonstrate that the deletion of these two genes significantly impairs the ability of <i>V. dahliae</i> to colonize the vascular bundles of plants and to form typical penetration pegs. Furthermore, transcriptomic analysis suggests that VdBre1 governs the lipid metabolism pathway, while VdHrd1 participates in endoplasmic-reticulum-related processes. Western blot analyses reveal a significant decrease in histone ubiquitination and histone H3K4 trimethylation levels in the <i>ΔVdBre1</i> mutant. This research illuminates the function of ubiquitin ligase E3 in <i>V. dahliae</i> and offers fresh theoretical perspectives. Our research identifies two novel virulence-related genes and partially explicates their roles in virulence-associated structures and gene regulatory pathways. These findings augment our understanding of the molecular mechanisms inherent to <i>V. dahliae</i>.https://www.mdpi.com/2309-608X/9/10/1037<i>Verticillium dahliae</i>ubiquitin ligase E3histone ubiquitinationVdBre1VdHrd1 |
| spellingShingle | Xing Yang Zhijuan Hu Jingjie Yuan Run Zou Yilan Wang Xuan Peng Shan Xu Chengjian Xie Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i> Journal of Fungi <i>Verticillium dahliae</i> ubiquitin ligase E3 histone ubiquitination VdBre1 VdHrd1 |
| title | Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i> |
| title_full | Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i> |
| title_fullStr | Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i> |
| title_full_unstemmed | Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i> |
| title_short | Functional Role of RING Ubiquitin E3 Ligase VdBre1 and VdHrd1 in the Pathogenicity and Penetration Structure Formation of <i>Verticillium dahliae</i> |
| title_sort | functional role of ring ubiquitin e3 ligase vdbre1 and vdhrd1 in the pathogenicity and penetration structure formation of i verticillium dahliae i |
| topic | <i>Verticillium dahliae</i> ubiquitin ligase E3 histone ubiquitination VdBre1 VdHrd1 |
| url | https://www.mdpi.com/2309-608X/9/10/1037 |
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