Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae
Phytopathogen Dickeya oryzae is a causal agent of rice foot rot disease and the pathogen has an array of virulence factors, such as phytotoxin zeamines, plant cell wall degrading enzymes, cell motility, and biofilms, collectively contributing to the bacterial pathogenesis. In this study, through del...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1033192/full |
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| author | Mingfa Lv Mingfa Lv Sixuan Ye Ming Hu Yang Xue Zhibin Liang Xiaofan Zhou Xiaofan Zhou Lianhui Zhang Lianhui Zhang Jianuan Zhou |
| author_facet | Mingfa Lv Mingfa Lv Sixuan Ye Ming Hu Yang Xue Zhibin Liang Xiaofan Zhou Xiaofan Zhou Lianhui Zhang Lianhui Zhang Jianuan Zhou |
| author_sort | Mingfa Lv |
| collection | DOAJ |
| description | Phytopathogen Dickeya oryzae is a causal agent of rice foot rot disease and the pathogen has an array of virulence factors, such as phytotoxin zeamines, plant cell wall degrading enzymes, cell motility, and biofilms, collectively contributing to the bacterial pathogenesis. In this study, through deletion analysis of predicted regulatory genes in D. oryzae EC1, we identified a two-component system associated with the regulation of bacterial virulence. The two-component system contains a histidine kinase ArcB and a response regulator ArcA, and deletion of their coding genes resulted in changed phenotypes in cell motility, biofilm formation, and bacterial virulence. Electrophoretic mobility shift assay revealed that ArcA bound to the promoters of the bcs operon and bssS, which respectively encode enzymes for the synthesis of celluloses and a biofilm formation regulatory protein. ArcA could also bind to the promoters of three virulence associated transcriptional regulatory genes, i.e., fis, slyA and ohrR. Surprisingly, although these three regulators were shown to modulate the production of cell wall degrading enzymes and zeamines, deletion of arcB and arcA did not seem to affect these phenotypes. Taken together, the findings from this study unveiled a new two-component system associated with the bacterial pathogenesis, which contributes to the virulence of D. oryzae mainly through its action on bacterial motility and biofilm formation. |
| first_indexed | 2024-04-13T19:48:59Z |
| format | Article |
| id | doaj.art-3149585895d9413d9a5d2ecc362c3ffe |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T19:48:59Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3149585895d9413d9a5d2ecc362c3ffe2022-12-22T02:32:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10331921033192Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzaeMingfa Lv0Mingfa Lv1Sixuan Ye2Ming Hu3Yang Xue4Zhibin Liang5Xiaofan Zhou6Xiaofan Zhou7Lianhui Zhang8Lianhui Zhang9Jianuan Zhou10Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaCollege of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong Province, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong Province, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, ChinaPhytopathogen Dickeya oryzae is a causal agent of rice foot rot disease and the pathogen has an array of virulence factors, such as phytotoxin zeamines, plant cell wall degrading enzymes, cell motility, and biofilms, collectively contributing to the bacterial pathogenesis. In this study, through deletion analysis of predicted regulatory genes in D. oryzae EC1, we identified a two-component system associated with the regulation of bacterial virulence. The two-component system contains a histidine kinase ArcB and a response regulator ArcA, and deletion of their coding genes resulted in changed phenotypes in cell motility, biofilm formation, and bacterial virulence. Electrophoretic mobility shift assay revealed that ArcA bound to the promoters of the bcs operon and bssS, which respectively encode enzymes for the synthesis of celluloses and a biofilm formation regulatory protein. ArcA could also bind to the promoters of three virulence associated transcriptional regulatory genes, i.e., fis, slyA and ohrR. Surprisingly, although these three regulators were shown to modulate the production of cell wall degrading enzymes and zeamines, deletion of arcB and arcA did not seem to affect these phenotypes. Taken together, the findings from this study unveiled a new two-component system associated with the bacterial pathogenesis, which contributes to the virulence of D. oryzae mainly through its action on bacterial motility and biofilm formation.https://www.frontiersin.org/articles/10.3389/fpls.2022.1033192/fullDickeya oryzaetwo-component systembiofilmmotilityvirulence |
| spellingShingle | Mingfa Lv Mingfa Lv Sixuan Ye Ming Hu Yang Xue Zhibin Liang Xiaofan Zhou Xiaofan Zhou Lianhui Zhang Lianhui Zhang Jianuan Zhou Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae Frontiers in Plant Science Dickeya oryzae two-component system biofilm motility virulence |
| title | Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae |
| title_full | Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae |
| title_fullStr | Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae |
| title_full_unstemmed | Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae |
| title_short | Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae |
| title_sort | two component system arcba modulates cell motility and biofilm formation in dickeya oryzae |
| topic | Dickeya oryzae two-component system biofilm motility virulence |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1033192/full |
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