Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection.
<h4>Background</h4>Undernutrition and schistosomiasis are public health problems and often occur in low and middle-income countries. Protein undernutrition can alter the host-parasite environment system and aggravate the course of schistosomiasis. This study aimed to assess the impact of...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-07-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0010249 |
| _version_ | 1798042309369004032 |
|---|---|
| author | Joseph Bertin Kadji Fassi Hermine Boukeng Jatsa Ulrich Membe Femoe Valentin Greigert Julie Brunet Catherine Cannet Christian Mérimé Kenfack Nestor Gipwe Feussom Emilienne Tienga Nkondo Ahmed Abou-Bacar Alexander Wilhelm Pfaff René Kamgang Pierre Kamtchouing Louis-Albert Tchuem Tchuenté |
| author_facet | Joseph Bertin Kadji Fassi Hermine Boukeng Jatsa Ulrich Membe Femoe Valentin Greigert Julie Brunet Catherine Cannet Christian Mérimé Kenfack Nestor Gipwe Feussom Emilienne Tienga Nkondo Ahmed Abou-Bacar Alexander Wilhelm Pfaff René Kamgang Pierre Kamtchouing Louis-Albert Tchuem Tchuenté |
| author_sort | Joseph Bertin Kadji Fassi |
| collection | DOAJ |
| description | <h4>Background</h4>Undernutrition and schistosomiasis are public health problems and often occur in low and middle-income countries. Protein undernutrition can alter the host-parasite environment system and aggravate the course of schistosomiasis. This study aimed to assess the impact of a low-protein diet on the efficacy of praziquantel.<h4>Methodology/principal findings</h4>Thirty-day-old mice were fed with a low-protein diet, and 40 days later, they were individually infected with fifty Schistosoma mansoni cercariae. A 28-day-treatment with praziquantel at 100 mg/kg for five consecutive days followed by distilled water begins on the 36th day post-infection. Mice were sacrificed on the 64th day post-infection. We determined the parasitological burden, liver and intestine histomorphometry, liver injury, and immunomodulation parameters. Praziquantel treatment of infected mice fed with a standard diet (IN-PZQ) resulted in a significant reduction of worm and egg burdens and a normalization of iron and calcium levels. The therapy also improved schistosomiasis-induced hepatopathy and oxidative stress. The anti-inflammatory and immunomodulatory activities of praziquantel were also significant in these mice. When infected mice receiving the low-protein diet were treated with praziquantel (ILP-PZQ), the body weight loss and hepatomegaly were not alleviated, and the worm and liver egg burdens were significantly higher than those of IN-PZQ mice (P < 0.001). The treatment did not reduce the increased activities of ALT and γ-GGT, the high malondialdehyde concentration, and the liver granuloma volume. The iron and calcium levels were not ameliorated and differed from those of IN-PZQ mice (P < 0.001 and P < 0.05). Moreover, in these mice, praziquantel treatment did not reverse the high level of IL-5 and the low mRNA expression of CCL3/MIP-1α and CXCL-10/IP-10 induced by S. mansoni infection.<h4>Conclusion/significance</h4>These results demonstrated that a low-protein diet reduced the schistosomicidal, antioxidant, anti-inflammatory, and immunomodulatory activities of praziquantel. |
| first_indexed | 2024-04-11T22:33:44Z |
| format | Article |
| id | doaj.art-3149d5eff8d84c358fcf90244b13afbd |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-11T22:33:44Z |
| publishDate | 2022-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-3149d5eff8d84c358fcf90244b13afbd2022-12-22T03:59:16ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-07-01167e001024910.1371/journal.pntd.0010249Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection.Joseph Bertin Kadji FassiHermine Boukeng JatsaUlrich Membe FemoeValentin GreigertJulie BrunetCatherine CannetChristian Mérimé KenfackNestor Gipwe FeussomEmilienne Tienga NkondoAhmed Abou-BacarAlexander Wilhelm PfaffRené KamgangPierre KamtchouingLouis-Albert Tchuem Tchuenté<h4>Background</h4>Undernutrition and schistosomiasis are public health problems and often occur in low and middle-income countries. Protein undernutrition can alter the host-parasite environment system and aggravate the course of schistosomiasis. This study aimed to assess the impact of a low-protein diet on the efficacy of praziquantel.<h4>Methodology/principal findings</h4>Thirty-day-old mice were fed with a low-protein diet, and 40 days later, they were individually infected with fifty Schistosoma mansoni cercariae. A 28-day-treatment with praziquantel at 100 mg/kg for five consecutive days followed by distilled water begins on the 36th day post-infection. Mice were sacrificed on the 64th day post-infection. We determined the parasitological burden, liver and intestine histomorphometry, liver injury, and immunomodulation parameters. Praziquantel treatment of infected mice fed with a standard diet (IN-PZQ) resulted in a significant reduction of worm and egg burdens and a normalization of iron and calcium levels. The therapy also improved schistosomiasis-induced hepatopathy and oxidative stress. The anti-inflammatory and immunomodulatory activities of praziquantel were also significant in these mice. When infected mice receiving the low-protein diet were treated with praziquantel (ILP-PZQ), the body weight loss and hepatomegaly were not alleviated, and the worm and liver egg burdens were significantly higher than those of IN-PZQ mice (P < 0.001). The treatment did not reduce the increased activities of ALT and γ-GGT, the high malondialdehyde concentration, and the liver granuloma volume. The iron and calcium levels were not ameliorated and differed from those of IN-PZQ mice (P < 0.001 and P < 0.05). Moreover, in these mice, praziquantel treatment did not reverse the high level of IL-5 and the low mRNA expression of CCL3/MIP-1α and CXCL-10/IP-10 induced by S. mansoni infection.<h4>Conclusion/significance</h4>These results demonstrated that a low-protein diet reduced the schistosomicidal, antioxidant, anti-inflammatory, and immunomodulatory activities of praziquantel.https://doi.org/10.1371/journal.pntd.0010249 |
| spellingShingle | Joseph Bertin Kadji Fassi Hermine Boukeng Jatsa Ulrich Membe Femoe Valentin Greigert Julie Brunet Catherine Cannet Christian Mérimé Kenfack Nestor Gipwe Feussom Emilienne Tienga Nkondo Ahmed Abou-Bacar Alexander Wilhelm Pfaff René Kamgang Pierre Kamtchouing Louis-Albert Tchuem Tchuenté Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection. PLoS Neglected Tropical Diseases |
| title | Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection. |
| title_full | Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection. |
| title_fullStr | Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection. |
| title_full_unstemmed | Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection. |
| title_short | Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection. |
| title_sort | protein undernutrition reduces the efficacy of praziquantel in a murine model of schistosoma mansoni infection |
| url | https://doi.org/10.1371/journal.pntd.0010249 |
| work_keys_str_mv | AT josephbertinkadjifassi proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT hermineboukengjatsa proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT ulrichmembefemoe proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT valentingreigert proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT juliebrunet proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT catherinecannet proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT christianmerimekenfack proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT nestorgipwefeussom proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT emiliennetiengankondo proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT ahmedaboubacar proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT alexanderwilhelmpfaff proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT renekamgang proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT pierrekamtchouing proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection AT louisalberttchuemtchuente proteinundernutritionreducestheefficacyofpraziquantelinamurinemodelofschistosomamansoniinfection |