| Summary: | Accurate determination of Cu<sup>2+</sup> in solution is crucial for preventing several disease conditions. Spectroscopy-based techniques for metal ion detection are promising methods due to their excellent sensitivity and rapid response time. In this work, we are reporting a newly synthesized 2-(2′-Hydroxyphenyl) benzoxazole-based compound, probe <b>2,</b> by incorporating a vinyl pyridinium segment into the <i>bis</i>(HBO) <b>4</b> system. Probe <b>2</b> exhibited excellent specificity toward Cu<sup>2+</sup> in solution. The ratiometric absorbance (λ<sub>440</sub>/λ<sub>370</sub>) and the quenching of fluorescence at λ<sub>em</sub> ≈585 nm exhibited an excellent linear correlation. The formation of the <b>2-Cu</b> complex can be utilized as a highly sensitive spectroscopic method for the detection of Cu<sup>2+</sup> in solution with a detection limit of 0.15 µM. In addition, Cu<sup>2+</sup>-induced fluorescence quenching in probe <b>2</b> occurs mainly via a static quenching mechanism by forming a <b>2-Cu</b> complex, and the stability constant for the <b>2-Cu</b> complex was calculated based on spectroscopic measurements.
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