Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution
Accurate determination of Cu<sup>2+</sup> in solution is crucial for preventing several disease conditions. Spectroscopy-based techniques for metal ion detection are promising methods due to their excellent sensitivity and rapid response time. In this work, we are reporting a newly synth...
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MDPI AG
2022-09-01
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| author | Buddhima U. Rajapakshe Yonghao Li Brian Corbin Kaveesha J. Wijesinghe Yi Pang Chathura S. Abeywickrama |
| author_facet | Buddhima U. Rajapakshe Yonghao Li Brian Corbin Kaveesha J. Wijesinghe Yi Pang Chathura S. Abeywickrama |
| author_sort | Buddhima U. Rajapakshe |
| collection | DOAJ |
| description | Accurate determination of Cu<sup>2+</sup> in solution is crucial for preventing several disease conditions. Spectroscopy-based techniques for metal ion detection are promising methods due to their excellent sensitivity and rapid response time. In this work, we are reporting a newly synthesized 2-(2′-Hydroxyphenyl) benzoxazole-based compound, probe <b>2,</b> by incorporating a vinyl pyridinium segment into the <i>bis</i>(HBO) <b>4</b> system. Probe <b>2</b> exhibited excellent specificity toward Cu<sup>2+</sup> in solution. The ratiometric absorbance (λ<sub>440</sub>/λ<sub>370</sub>) and the quenching of fluorescence at λ<sub>em</sub> ≈585 nm exhibited an excellent linear correlation. The formation of the <b>2-Cu</b> complex can be utilized as a highly sensitive spectroscopic method for the detection of Cu<sup>2+</sup> in solution with a detection limit of 0.15 µM. In addition, Cu<sup>2+</sup>-induced fluorescence quenching in probe <b>2</b> occurs mainly via a static quenching mechanism by forming a <b>2-Cu</b> complex, and the stability constant for the <b>2-Cu</b> complex was calculated based on spectroscopic measurements. |
| first_indexed | 2024-03-09T20:27:59Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T20:27:59Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-314fd009bd0f4ab58a5d2cf2051478d22023-11-23T23:29:46ZengMDPI AGChemosensors2227-90402022-09-01101038210.3390/chemosensors10100382Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in SolutionBuddhima U. Rajapakshe0Yonghao Li1Brian Corbin2Kaveesha J. Wijesinghe3Yi Pang4Chathura S. Abeywickrama5Department of Chemistry, Faculty of Science, University of Colombo, Colombo 00700, Sri LankaDepartment of Chemistry, The University of Akron, Akron, OH 44325, USADepartment of Chemistry, The University of Akron, Akron, OH 44325, USADepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 00700, Sri LankaDepartment of Chemistry, The University of Akron, Akron, OH 44325, USADepartment of Chemistry, The University of Akron, Akron, OH 44325, USAAccurate determination of Cu<sup>2+</sup> in solution is crucial for preventing several disease conditions. Spectroscopy-based techniques for metal ion detection are promising methods due to their excellent sensitivity and rapid response time. In this work, we are reporting a newly synthesized 2-(2′-Hydroxyphenyl) benzoxazole-based compound, probe <b>2,</b> by incorporating a vinyl pyridinium segment into the <i>bis</i>(HBO) <b>4</b> system. Probe <b>2</b> exhibited excellent specificity toward Cu<sup>2+</sup> in solution. The ratiometric absorbance (λ<sub>440</sub>/λ<sub>370</sub>) and the quenching of fluorescence at λ<sub>em</sub> ≈585 nm exhibited an excellent linear correlation. The formation of the <b>2-Cu</b> complex can be utilized as a highly sensitive spectroscopic method for the detection of Cu<sup>2+</sup> in solution with a detection limit of 0.15 µM. In addition, Cu<sup>2+</sup>-induced fluorescence quenching in probe <b>2</b> occurs mainly via a static quenching mechanism by forming a <b>2-Cu</b> complex, and the stability constant for the <b>2-Cu</b> complex was calculated based on spectroscopic measurements.https://www.mdpi.com/2227-9040/10/10/382fluorescent dyes2-(2′-hydroxyphenyl)benzoxazole (HBO)excited-state intramolecular proton transfer (ESIPT)fluorescence quenchingcopper ion |
| spellingShingle | Buddhima U. Rajapakshe Yonghao Li Brian Corbin Kaveesha J. Wijesinghe Yi Pang Chathura S. Abeywickrama Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution Chemosensors fluorescent dyes 2-(2′-hydroxyphenyl)benzoxazole (HBO) excited-state intramolecular proton transfer (ESIPT) fluorescence quenching copper ion |
| title | Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution |
| title_full | Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution |
| title_fullStr | Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution |
| title_full_unstemmed | Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution |
| title_short | Copper-Induced Fluorescence Quenching in a <i>Bis</i>[2-(2′-hydroxyphenyl)benzoxazole]pyridinium Derivative for Quantification of Cu<sup>2+</sup> in Solution |
| title_sort | copper induced fluorescence quenching in a i bis i 2 2 hydroxyphenyl benzoxazole pyridinium derivative for quantification of cu sup 2 sup in solution |
| topic | fluorescent dyes 2-(2′-hydroxyphenyl)benzoxazole (HBO) excited-state intramolecular proton transfer (ESIPT) fluorescence quenching copper ion |
| url | https://www.mdpi.com/2227-9040/10/10/382 |
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