Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields
Cadmium pollution is becoming a serious problem due to its nondegradability and substantial negative influence on the normal growth of crops, thereby harming human health through the food chain. Rhizospheric bacteria play important roles in crop tolerance. However, there is little experimental evide...
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PeerJ Inc.
2020-11-01
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| author | Li Song Zhenzhi Pan Yi Dai Lin Chen Li Zhang Qilin Liao Xiezhi Yu Hongyan Guo Guisheng Zhou |
| author_facet | Li Song Zhenzhi Pan Yi Dai Lin Chen Li Zhang Qilin Liao Xiezhi Yu Hongyan Guo Guisheng Zhou |
| author_sort | Li Song |
| collection | DOAJ |
| description | Cadmium pollution is becoming a serious problem due to its nondegradability and substantial negative influence on the normal growth of crops, thereby harming human health through the food chain. Rhizospheric bacteria play important roles in crop tolerance. However, there is little experimental evidence which demonstrates how various cadmium concentrations affect the bacterial community in wheat fields including rhizosphere microorganisms and nonrhizosphere (bulk) microorganisms. In this study, 16S rRNA amplicon sequencing technology was used to investigate bacterial communities in rhizosphere and bulk soils under different levels of pollution in terms of cadmium concentration. Both the richness and diversity of the rhizosphere microorganism community were higher under nonpolluted soil and very mild and mild cadmium-contaminated soils than compared with bulk soil, with a shift in community profile observed under severe cadmium pollution. Moreover, cadmium at various concentrations had greater influence on bacterial composition than for the nonpolluted site. In addition, redundancy analysis (RDA) and Spearman’s analysis elucidated the impact of exchangeable Cd and total Cd on bacterial community abundance and composition. This study suggests that cadmium imposes a distinct effect on bacterial community, both in bulk and rhizosphere soils of wheat fields. This study increases our understanding of how bacterial communities in wheat fields shaped under different concentrations of cadmium. |
| first_indexed | 2024-03-09T08:04:14Z |
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| id | doaj.art-3150b010d57f41afb37ab91e5688f966 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:04:14Z |
| publishDate | 2020-11-01 |
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| spelling | doaj.art-3150b010d57f41afb37ab91e5688f9662023-12-03T00:23:53ZengPeerJ Inc.PeerJ2167-83592020-11-018e1030210.7717/peerj.10302Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fieldsLi Song0Zhenzhi Pan1Yi Dai2Lin Chen3Li Zhang4Qilin Liao5Xiezhi Yu6Hongyan Guo7Guisheng Zhou8Joint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu, ChinaState Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing Univerity, Nanjing, Jiangsu, ChinaGeological Survey of Jiangsu Province, Nanjing, Jiangsu, ChinaState Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing Univerity, Nanjing, Jiangsu, ChinaState Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing Univerity, Nanjing, Jiangsu, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, Jiangsu, ChinaCadmium pollution is becoming a serious problem due to its nondegradability and substantial negative influence on the normal growth of crops, thereby harming human health through the food chain. Rhizospheric bacteria play important roles in crop tolerance. However, there is little experimental evidence which demonstrates how various cadmium concentrations affect the bacterial community in wheat fields including rhizosphere microorganisms and nonrhizosphere (bulk) microorganisms. In this study, 16S rRNA amplicon sequencing technology was used to investigate bacterial communities in rhizosphere and bulk soils under different levels of pollution in terms of cadmium concentration. Both the richness and diversity of the rhizosphere microorganism community were higher under nonpolluted soil and very mild and mild cadmium-contaminated soils than compared with bulk soil, with a shift in community profile observed under severe cadmium pollution. Moreover, cadmium at various concentrations had greater influence on bacterial composition than for the nonpolluted site. In addition, redundancy analysis (RDA) and Spearman’s analysis elucidated the impact of exchangeable Cd and total Cd on bacterial community abundance and composition. This study suggests that cadmium imposes a distinct effect on bacterial community, both in bulk and rhizosphere soils of wheat fields. This study increases our understanding of how bacterial communities in wheat fields shaped under different concentrations of cadmium.https://peerj.com/articles/10302.pdfWheat16S rRNA amplicon sequencing CadmiumMicrobial communityRhizosphere |
| spellingShingle | Li Song Zhenzhi Pan Yi Dai Lin Chen Li Zhang Qilin Liao Xiezhi Yu Hongyan Guo Guisheng Zhou Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields PeerJ Wheat 16S rRNA amplicon sequencing Cadmium Microbial community Rhizosphere |
| title | Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields |
| title_full | Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields |
| title_fullStr | Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields |
| title_full_unstemmed | Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields |
| title_short | Characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium-polluted wheat fields |
| title_sort | characterization and comparison of the bacterial communities of rhizosphere and bulk soils from cadmium polluted wheat fields |
| topic | Wheat 16S rRNA amplicon sequencing Cadmium Microbial community Rhizosphere |
| url | https://peerj.com/articles/10302.pdf |
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