Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology.
COVID-19 caused by the SARS-CoV-2 virus is widespread in all regions, and it disturbs host immune system functioning leading to extreme inflammatory reaction and hyperactivation of the immune response. Kabasura Kudineer (KSK) is preventive medicine against viral infections and a potent immune booste...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0282263 |
| _version_ | 1797843031209017344 |
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| author | Vidhya Rajalakshmi V Akilandeswari Ramu Jayaprakash Chinnappan Palanivel Velmurugan Rajiv Pathak Rami Adel Pashameah Atif Abdulwahab A Oyouni Osama M Al-Amer Mohammed I Alasseiri Abdullah Hamadi Mansuor A Alanazi Thangavelu Sathiamoorthi |
| author_facet | Vidhya Rajalakshmi V Akilandeswari Ramu Jayaprakash Chinnappan Palanivel Velmurugan Rajiv Pathak Rami Adel Pashameah Atif Abdulwahab A Oyouni Osama M Al-Amer Mohammed I Alasseiri Abdullah Hamadi Mansuor A Alanazi Thangavelu Sathiamoorthi |
| author_sort | Vidhya Rajalakshmi V |
| collection | DOAJ |
| description | COVID-19 caused by the SARS-CoV-2 virus is widespread in all regions, and it disturbs host immune system functioning leading to extreme inflammatory reaction and hyperactivation of the immune response. Kabasura Kudineer (KSK) is preventive medicine against viral infections and a potent immune booster for inflammation-related diseases. We hypothesize that KSK and KSK similar plant compounds, might prevent or control the COVID-19 infection in the human body. 1,207 KSK and KSK similar compounds were listed and screened via the Swiss ADME tool and PAINS Remover; 303 compounds were filtered including active and similar drug compounds. The targets were retrieved from similar drugs of the active compounds of KSK. Finally, 573 genes were listed after several screening steps. Next, network analysis was performed to finalize the potential target gene: construction of protein-protein interaction of 573 genes using STRING, identifying top hub genes in Cytoscape plug-ins (MCODE and cytoHubba). These ten hub genes play a crucial role in the inflammatory response. Target-miRNA interaction was also constructed using the miRNet tool to interpret miRNAs of the target genes and their functions. Functional annotation was done via DAVID to gain a complete insight into the mechanism of the enriched pathways and other diseases related to the given target genes. In Molecular Docking analysis, IL10 attained top rank in Target-miRNA interaction and also the gene formed prominent exchanges with an excellent binding score (> = -8.0) against 19 compounds. Among them, Guggulsterone has an acute affinity score of -8.8 for IL10 and exhibits anti-inflammatory and immunomodulatory properties. Molecular Dynamics simulation study also performed for IL10 and the interacting ligand compounds using GROMACS. Finally, Guggulsterone will be recommended to enhance immunity against several inflammatory diseases, including COVID19. |
| first_indexed | 2024-04-09T16:57:52Z |
| format | Article |
| id | doaj.art-3153d7b438474ccf84e04af9e6b06050 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T16:57:52Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3153d7b438474ccf84e04af9e6b060502023-04-21T05:34:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01183e028226310.1371/journal.pone.0282263Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology.Vidhya Rajalakshmi VAkilandeswari RamuJayaprakash ChinnappanPalanivel VelmuruganRajiv PathakRami Adel PashameahAtif Abdulwahab A OyouniOsama M Al-AmerMohammed I AlasseiriAbdullah HamadiMansuor A AlanaziThangavelu SathiamoorthiCOVID-19 caused by the SARS-CoV-2 virus is widespread in all regions, and it disturbs host immune system functioning leading to extreme inflammatory reaction and hyperactivation of the immune response. Kabasura Kudineer (KSK) is preventive medicine against viral infections and a potent immune booster for inflammation-related diseases. We hypothesize that KSK and KSK similar plant compounds, might prevent or control the COVID-19 infection in the human body. 1,207 KSK and KSK similar compounds were listed and screened via the Swiss ADME tool and PAINS Remover; 303 compounds were filtered including active and similar drug compounds. The targets were retrieved from similar drugs of the active compounds of KSK. Finally, 573 genes were listed after several screening steps. Next, network analysis was performed to finalize the potential target gene: construction of protein-protein interaction of 573 genes using STRING, identifying top hub genes in Cytoscape plug-ins (MCODE and cytoHubba). These ten hub genes play a crucial role in the inflammatory response. Target-miRNA interaction was also constructed using the miRNet tool to interpret miRNAs of the target genes and their functions. Functional annotation was done via DAVID to gain a complete insight into the mechanism of the enriched pathways and other diseases related to the given target genes. In Molecular Docking analysis, IL10 attained top rank in Target-miRNA interaction and also the gene formed prominent exchanges with an excellent binding score (> = -8.0) against 19 compounds. Among them, Guggulsterone has an acute affinity score of -8.8 for IL10 and exhibits anti-inflammatory and immunomodulatory properties. Molecular Dynamics simulation study also performed for IL10 and the interacting ligand compounds using GROMACS. Finally, Guggulsterone will be recommended to enhance immunity against several inflammatory diseases, including COVID19.https://doi.org/10.1371/journal.pone.0282263 |
| spellingShingle | Vidhya Rajalakshmi V Akilandeswari Ramu Jayaprakash Chinnappan Palanivel Velmurugan Rajiv Pathak Rami Adel Pashameah Atif Abdulwahab A Oyouni Osama M Al-Amer Mohammed I Alasseiri Abdullah Hamadi Mansuor A Alanazi Thangavelu Sathiamoorthi Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology. PLoS ONE |
| title | Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology. |
| title_full | Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology. |
| title_fullStr | Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology. |
| title_full_unstemmed | Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology. |
| title_short | Interleukin-10 as Covid-19 biomarker targeting KSK and its analogues: Integrated network pharmacology. |
| title_sort | interleukin 10 as covid 19 biomarker targeting ksk and its analogues integrated network pharmacology |
| url | https://doi.org/10.1371/journal.pone.0282263 |
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