Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling
Coupled with an azo coupling reaction, a simple, rapid, sensitive, and effective surface-enhanced resonance Raman scattering (SERRS) detection method for benzocaine was developed. In our study, benzocaine which is used clinically as a local anesthetic was derived with <i>p</i>-aminothiop...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/14/4492 |
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| author | Chao-Yang Zhao Huimin Sui Endi Xue Li Li Jie Zhang Tao Xu Xin Liang Ying Yang |
| author_facet | Chao-Yang Zhao Huimin Sui Endi Xue Li Li Jie Zhang Tao Xu Xin Liang Ying Yang |
| author_sort | Chao-Yang Zhao |
| collection | DOAJ |
| description | Coupled with an azo coupling reaction, a simple, rapid, sensitive, and effective surface-enhanced resonance Raman scattering (SERRS) detection method for benzocaine was developed. In our study, benzocaine which is used clinically as a local anesthetic was derived with <i>p</i>-aminothiophenol into a corresponding azo product within 5 min, resulting in a strong SERRS response with the simple addition of Ag NPs excited with a 532 nm laser. The linear correlation between SERRS intensity of dominant bands and logarithm of benzocaine concentration was investigated for quantitative determination. The method reached a limit of detection (<i>LOD</i>) down to 0.139 and 0.0788 μg/mL calculated with two peak intensity ratios (<i>I</i><sub>1568</sub>/<i>I</i><sub>2260</sub> and <i>I</i><sub>1331</sub>/<i>I</i><sub>2260</sub>), which is comparable to most studies reported previously, and meanwhile had superiority in simplicity and rapidness. The quantitative measurements for pharmaceutical preparations with benzocaine were conducted without complex extraction and enrichment processes. It was indicated that the SERRS assay combined with azo derivatization reaction has implications for practical applications in more complicated systems involving biological samples, in which appropriate and simplified pretreatments were conducted to remove interfering components. |
| first_indexed | 2024-03-09T10:14:10Z |
| format | Article |
| id | doaj.art-3157369729b94f61b9553d1dc90ecbb2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:14:10Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3157369729b94f61b9553d1dc90ecbb22023-12-01T22:29:55ZengMDPI AGMolecules1420-30492022-07-012714449210.3390/molecules27144492Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo CouplingChao-Yang Zhao0Huimin Sui1Endi Xue2Li Li3Jie Zhang4Tao Xu5Xin Liang6Ying Yang7School of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaSchool of Pharmacy, Qiqihar Medical University, Qiqihar 161000, ChinaCoupled with an azo coupling reaction, a simple, rapid, sensitive, and effective surface-enhanced resonance Raman scattering (SERRS) detection method for benzocaine was developed. In our study, benzocaine which is used clinically as a local anesthetic was derived with <i>p</i>-aminothiophenol into a corresponding azo product within 5 min, resulting in a strong SERRS response with the simple addition of Ag NPs excited with a 532 nm laser. The linear correlation between SERRS intensity of dominant bands and logarithm of benzocaine concentration was investigated for quantitative determination. The method reached a limit of detection (<i>LOD</i>) down to 0.139 and 0.0788 μg/mL calculated with two peak intensity ratios (<i>I</i><sub>1568</sub>/<i>I</i><sub>2260</sub> and <i>I</i><sub>1331</sub>/<i>I</i><sub>2260</sub>), which is comparable to most studies reported previously, and meanwhile had superiority in simplicity and rapidness. The quantitative measurements for pharmaceutical preparations with benzocaine were conducted without complex extraction and enrichment processes. It was indicated that the SERRS assay combined with azo derivatization reaction has implications for practical applications in more complicated systems involving biological samples, in which appropriate and simplified pretreatments were conducted to remove interfering components.https://www.mdpi.com/1420-3049/27/14/4492benzocaineSERRSazo couplingderivatizationRaman |
| spellingShingle | Chao-Yang Zhao Huimin Sui Endi Xue Li Li Jie Zhang Tao Xu Xin Liang Ying Yang Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling Molecules benzocaine SERRS azo coupling derivatization Raman |
| title | Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling |
| title_full | Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling |
| title_fullStr | Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling |
| title_full_unstemmed | Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling |
| title_short | Determination of Benzocaine in Pharmaceutical Formulations by Indirect SERRS Assay Combined with Azo Coupling |
| title_sort | determination of benzocaine in pharmaceutical formulations by indirect serrs assay combined with azo coupling |
| topic | benzocaine SERRS azo coupling derivatization Raman |
| url | https://www.mdpi.com/1420-3049/27/14/4492 |
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