Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol
Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species <i>Yarrowia lipolytica</i...
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| author | Anita Rywińska Ludwika Tomaszewska-Hetman Zbigniew Lazar Piotr Juszczyk Patrycja Sałata Karolina Malek Adrian Kawecki Waldemar Rymowicz |
| author_facet | Anita Rywińska Ludwika Tomaszewska-Hetman Zbigniew Lazar Piotr Juszczyk Patrycja Sałata Karolina Malek Adrian Kawecki Waldemar Rymowicz |
| author_sort | Anita Rywińska |
| collection | DOAJ |
| description | Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species <i>Yarrowia lipolytica</i>. The aim of this study was to evaluate transformants of <i>Y. lipolytica</i> for growth and ability to overproduce citric acids and erythritol from glycerol. The transformants were constructed by overexpressing glycerol kinase, methylcitrate synthase and mitochondrial succinate-fumarate transporter in the mutant Wratislavia 1.31. Next, strains were assessed for biosynthesis of citrate (pH 5.5; nitrogen limitation) and erythritol (pH 3.0; high osmotic pressure) from glycerol. Regardless of culture conditions strains, 1.31.GUT1/6 and 1.31.GUT1/6.CIT1/3 exhibited high rates of substrate utilization. Under conditions favoring citrate biosynthesis, both strains produced several percent more citrates, accompanied by higher erythritol production compared to the parental strain. During erythritol biosynthesis, the strain 1.31.GUT1/6.CIT1/3.E34672g obtained as a result of co-expression of all three genes stood out, producing 84.0 g/L of erythritol with yield and productivity of 0.54 g/g and 0.72 g/Lh, respectively, which places it in the group of the highest-ranked producers of erythritol among <i>Y. lipolytica</i> species. |
| first_indexed | 2024-03-08T03:55:24Z |
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| language | English |
| last_indexed | 2024-03-08T03:55:24Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3158e4352a3647629566fe2f619292792024-02-09T15:13:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253147510.3390/ijms25031475Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from GlycerolAnita Rywińska0Ludwika Tomaszewska-Hetman1Zbigniew Lazar2Piotr Juszczyk3Patrycja Sałata4Karolina Malek5Adrian Kawecki6Waldemar Rymowicz7Department of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, The Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, Chełmońskiego Str. 37, 51-630 Wrocław, PolandCitric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species <i>Yarrowia lipolytica</i>. The aim of this study was to evaluate transformants of <i>Y. lipolytica</i> for growth and ability to overproduce citric acids and erythritol from glycerol. The transformants were constructed by overexpressing glycerol kinase, methylcitrate synthase and mitochondrial succinate-fumarate transporter in the mutant Wratislavia 1.31. Next, strains were assessed for biosynthesis of citrate (pH 5.5; nitrogen limitation) and erythritol (pH 3.0; high osmotic pressure) from glycerol. Regardless of culture conditions strains, 1.31.GUT1/6 and 1.31.GUT1/6.CIT1/3 exhibited high rates of substrate utilization. Under conditions favoring citrate biosynthesis, both strains produced several percent more citrates, accompanied by higher erythritol production compared to the parental strain. During erythritol biosynthesis, the strain 1.31.GUT1/6.CIT1/3.E34672g obtained as a result of co-expression of all three genes stood out, producing 84.0 g/L of erythritol with yield and productivity of 0.54 g/g and 0.72 g/Lh, respectively, which places it in the group of the highest-ranked producers of erythritol among <i>Y. lipolytica</i> species.https://www.mdpi.com/1422-0067/25/3/1475<i>Yarrowia lipolytica</i>glycerolcitrateserythritolgenetic engineering |
| spellingShingle | Anita Rywińska Ludwika Tomaszewska-Hetman Zbigniew Lazar Piotr Juszczyk Patrycja Sałata Karolina Malek Adrian Kawecki Waldemar Rymowicz Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol International Journal of Molecular Sciences <i>Yarrowia lipolytica</i> glycerol citrates erythritol genetic engineering |
| title | Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol |
| title_full | Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol |
| title_fullStr | Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol |
| title_full_unstemmed | Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol |
| title_short | Application of New <i>Yarrowia lipolytica</i> Transformants in Production of Citrates and Erythritol from Glycerol |
| title_sort | application of new i yarrowia lipolytica i transformants in production of citrates and erythritol from glycerol |
| topic | <i>Yarrowia lipolytica</i> glycerol citrates erythritol genetic engineering |
| url | https://www.mdpi.com/1422-0067/25/3/1475 |
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