Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors
ABSTRACTSince the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, w...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1770129 |
| _version_ | 1827320683283087360 |
|---|---|
| author | Huan Han Qingfeng Ma Cong Li Rui Liu Li Zhao Wei Wang Pingan Zhang Xinghui Liu Guosheng Gao Fang Liu Yingan Jiang Xiaoming Cheng Chengliang Zhu Yuchen Xia |
| author_facet | Huan Han Qingfeng Ma Cong Li Rui Liu Li Zhao Wei Wang Pingan Zhang Xinghui Liu Guosheng Gao Fang Liu Yingan Jiang Xiaoming Cheng Chengliang Zhu Yuchen Xia |
| author_sort | Huan Han |
| collection | DOAJ |
| description | ABSTRACTSince the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe and critical groups according to their symptoms. 45 control samples of healthy volunteers were also included. Inflammatory cytokines and C-Reactive Protein (CRP) profiles of serum samples were analyzed by specific immunoassays. Results showed that COVID-19 patients have higher serum level of cytokines (TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10) and CRP than control individuals. Within COVID-19 patients, serum IL-6 and IL-10 levels are significantly higher in critical group (n = 17) than in moderate (n = 42) and severe (n = 43) group. The levels of IL-10 is positively correlated with CRP amount (r = 0.41, P < 0.01). Using univariate logistic regression analysis, IL-6 and IL-10 are found to be predictive of disease severity and receiver operating curve analysis could further confirm this result (AUC = 0.841, 0.822 respectively). Our result indicated higher levels of cytokine storm is associated with more severe disease development. Among them, IL-6 and IL-10 can be used as predictors for fast diagnosis of patients with higher risk of disease deterioration. Given the high levels of cytokines induced by SARS-CoV-2, treatment to reduce inflammation-related lung damage is critical. |
| first_indexed | 2024-03-07T17:23:49Z |
| format | Article |
| id | doaj.art-315ae824281549319ec3aa4307515560 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:51:28Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-315ae824281549319ec3aa43075155602024-03-11T16:04:24ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911123113010.1080/22221751.2020.1770129Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictorsHuan Han0Qingfeng Ma1Cong Li2Rui Liu3Li Zhao4Wei Wang5Pingan Zhang6Xinghui Liu7Guosheng Gao8Fang Liu9Yingan Jiang10Xiaoming Cheng11Chengliang Zhu12Yuchen Xia13Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of ChinaDepartment of Clinical Laboratory, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, Wuhan, People’s Republic of ChinaDepartment of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, Wuhan, People’s Republic of ChinaDepartment of Clinical Laboratory, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of ChinaDepartment of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of ChinaDepartment of Clinical Laboratory, Shanghai Gongli Hospital, the Second Military Medical University, Shanghai, People’s Republic of ChinaDepartment of Clinical Laboratory, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, People’s Republic of ChinaState Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of ChinaDepartment of Infectious Diseases, Renmin Hospital, Wuhan University, Wuhan, People’s Republic of ChinaLiver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USADepartment of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of ChinaState Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, Wuhan, People’s Republic of ChinaABSTRACTSince the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe and critical groups according to their symptoms. 45 control samples of healthy volunteers were also included. Inflammatory cytokines and C-Reactive Protein (CRP) profiles of serum samples were analyzed by specific immunoassays. Results showed that COVID-19 patients have higher serum level of cytokines (TNF-α, IFN-γ, IL-2, IL-4, IL-6 and IL-10) and CRP than control individuals. Within COVID-19 patients, serum IL-6 and IL-10 levels are significantly higher in critical group (n = 17) than in moderate (n = 42) and severe (n = 43) group. The levels of IL-10 is positively correlated with CRP amount (r = 0.41, P < 0.01). Using univariate logistic regression analysis, IL-6 and IL-10 are found to be predictive of disease severity and receiver operating curve analysis could further confirm this result (AUC = 0.841, 0.822 respectively). Our result indicated higher levels of cytokine storm is associated with more severe disease development. Among them, IL-6 and IL-10 can be used as predictors for fast diagnosis of patients with higher risk of disease deterioration. Given the high levels of cytokines induced by SARS-CoV-2, treatment to reduce inflammation-related lung damage is critical.https://www.tandfonline.com/doi/10.1080/22221751.2020.1770129SARS-CoV-2COVID-19cytokine storminflammatory cytokinesInterleukin 6Interleukin 10 |
| spellingShingle | Huan Han Qingfeng Ma Cong Li Rui Liu Li Zhao Wei Wang Pingan Zhang Xinghui Liu Guosheng Gao Fang Liu Yingan Jiang Xiaoming Cheng Chengliang Zhu Yuchen Xia Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors Emerging Microbes and Infections SARS-CoV-2 COVID-19 cytokine storm inflammatory cytokines Interleukin 6 Interleukin 10 |
| title | Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors |
| title_full | Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors |
| title_fullStr | Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors |
| title_full_unstemmed | Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors |
| title_short | Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors |
| title_sort | profiling serum cytokines in covid 19 patients reveals il 6 and il 10 are disease severity predictors |
| topic | SARS-CoV-2 COVID-19 cytokine storm inflammatory cytokines Interleukin 6 Interleukin 10 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1770129 |
| work_keys_str_mv | AT huanhan profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT qingfengma profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT congli profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT ruiliu profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT lizhao profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT weiwang profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT pinganzhang profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT xinghuiliu profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT guoshenggao profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT fangliu profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT yinganjiang profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT xiaomingcheng profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT chengliangzhu profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors AT yuchenxia profilingserumcytokinesincovid19patientsrevealsil6andil10arediseaseseveritypredictors |