<i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i>
In order to survive, plants have, over the course of their evolution, developed sophisticated acclimation and defense strategies governed by complex molecular and physiological, and cellular and extracellular, signaling pathways. They are also able to respond to various stimuli in the form of tropis...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13386 |
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| author | Jakub Mielecki Piotr Gawroński Stanisław Karpiński |
| author_facet | Jakub Mielecki Piotr Gawroński Stanisław Karpiński |
| author_sort | Jakub Mielecki |
| collection | DOAJ |
| description | In order to survive, plants have, over the course of their evolution, developed sophisticated acclimation and defense strategies governed by complex molecular and physiological, and cellular and extracellular, signaling pathways. They are also able to respond to various stimuli in the form of tropisms; for example, phototropism or gravitropism. All of these retrograde and anterograde signaling pathways are controlled and regulated by waves of reactive oxygen species (ROS), electrical signals, calcium, and hormones, e.g., auxins. Auxins are key phytohormones involved in the regulation of plant growth and development. Acclimation responses, which include programmed cell death induction, require precise auxin perception. However, our knowledge of these pathways is limited. The Aux/IAA family of transcriptional corepressors inhibits the growth of the plant under stress conditions, in order to maintain the balance between development and acclimation responses. In this work, we demonstrate the <i>Aux/IAA11</i> involvement in auxin sensing, survival, and acclimation to UV-AB, and in carrying out photosynthesis under inhibitory conditions. The tested <i>iaa11</i> mutants were more susceptible to UV-AB, photosynthetic electron transport (PET) inhibitor, and synthetic endogenous auxin. Among the tested conditions, <i>Aux/IAA11</i> was not repressed by excess light stress, exclusively among its phylogenetic clade. Repression of transcription by Aux/IAA11 could be important for the inhibition of ROS formation or efficiency of ROS scavenging. We also hypothesize that the demonstrated differences in the subcellular localization of the two Aux/IAA11 protein variants might indicate their regulation by alternative splicing. Our results suggest that <i>Aux/IAA11</i> plays a specific role in chloroplast retrograde signaling, since it is not repressed by high (excess) light stress, exclusively among its phylogenetic clade. |
| first_indexed | 2024-03-09T18:59:25Z |
| format | Article |
| id | doaj.art-315b4c32742248d5aaa8450eac6fcb9e |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:59:25Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-315b4c32742248d5aaa8450eac6fcb9e2023-11-24T05:07:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211338610.3390/ijms232113386<i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i>Jakub Mielecki0Piotr Gawroński1Stanisław Karpiński2Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandIn order to survive, plants have, over the course of their evolution, developed sophisticated acclimation and defense strategies governed by complex molecular and physiological, and cellular and extracellular, signaling pathways. They are also able to respond to various stimuli in the form of tropisms; for example, phototropism or gravitropism. All of these retrograde and anterograde signaling pathways are controlled and regulated by waves of reactive oxygen species (ROS), electrical signals, calcium, and hormones, e.g., auxins. Auxins are key phytohormones involved in the regulation of plant growth and development. Acclimation responses, which include programmed cell death induction, require precise auxin perception. However, our knowledge of these pathways is limited. The Aux/IAA family of transcriptional corepressors inhibits the growth of the plant under stress conditions, in order to maintain the balance between development and acclimation responses. In this work, we demonstrate the <i>Aux/IAA11</i> involvement in auxin sensing, survival, and acclimation to UV-AB, and in carrying out photosynthesis under inhibitory conditions. The tested <i>iaa11</i> mutants were more susceptible to UV-AB, photosynthetic electron transport (PET) inhibitor, and synthetic endogenous auxin. Among the tested conditions, <i>Aux/IAA11</i> was not repressed by excess light stress, exclusively among its phylogenetic clade. Repression of transcription by Aux/IAA11 could be important for the inhibition of ROS formation or efficiency of ROS scavenging. We also hypothesize that the demonstrated differences in the subcellular localization of the two Aux/IAA11 protein variants might indicate their regulation by alternative splicing. Our results suggest that <i>Aux/IAA11</i> plays a specific role in chloroplast retrograde signaling, since it is not repressed by high (excess) light stress, exclusively among its phylogenetic clade.https://www.mdpi.com/1422-0067/23/21/13386abiotic stress responsehormonal regulationreactive oxygen speciescell death |
| spellingShingle | Jakub Mielecki Piotr Gawroński Stanisław Karpiński <i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i> International Journal of Molecular Sciences abiotic stress response hormonal regulation reactive oxygen species cell death |
| title | <i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i> |
| title_full | <i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i> |
| title_fullStr | <i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | <i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i> |
| title_short | <i>Aux/IAA11</i> Is Required for UV-AB Tolerance and Auxin Sensing in <i>Arabidopsis thaliana</i> |
| title_sort | i aux iaa11 i is required for uv ab tolerance and auxin sensing in i arabidopsis thaliana i |
| topic | abiotic stress response hormonal regulation reactive oxygen species cell death |
| url | https://www.mdpi.com/1422-0067/23/21/13386 |
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