Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri
Abstract Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific histone, are involved in chromatin condensa...
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53729-2 |
| _version_ | 1797275060005765120 |
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| author | Yu Takeuchi Shinya Sato Chikako Nagasato Taizo Motomura Shujiro Okuda Masahiro Kasahara Fumio Takahashi Shinya Yoshikawa |
| author_facet | Yu Takeuchi Shinya Sato Chikako Nagasato Taizo Motomura Shujiro Okuda Masahiro Kasahara Fumio Takahashi Shinya Yoshikawa |
| author_sort | Yu Takeuchi |
| collection | DOAJ |
| description | Abstract Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific histone, are involved in chromatin condensation of the sperm nucleus. Among brown algae, sperm of the oogamous Fucales algae have a condensed nucleus. However, the existence of sperm-specific SNBPs in Fucales algae was unclear. Here, we identified linker histone (histone H1) proteins in the sperm and analyzed changes in their gene expression pattern during spermatogenesis in Sargassum horneri. A search of transcriptomic data for histone H1 genes in showed six histone H1 genes, which we named ShH1.1a, ShH1b, ShH1.2, ShH1.3, ShH1.4, and ShH1.5. Analysis of SNBPs using SDS-PAGE and LC–MS/MS showed that sperm nuclei contain histone ShH1.2, ShH1.3, and ShH1.4 in addition to core histones. Both ShH1.2 and ShH1.3 genes were expressed in the vegetative thallus and the male and female receptacles (the organs producing antheridium or oogonium). Meanwhile, the ShH1.4 gene was expressed in the male receptacle but not in the vegetative thallus and female receptacles. From these results, ShH1.4 may be a sperm-specific histone H1 of S. horneri. |
| first_indexed | 2024-03-07T15:08:54Z |
| format | Article |
| id | doaj.art-315b551a7ad544ef928a91dff5a8d988 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:08:54Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-315b551a7ad544ef928a91dff5a8d9882024-03-05T18:45:47ZengNature PortfolioScientific Reports2045-23222024-02-0114111310.1038/s41598-024-53729-2Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneriYu Takeuchi0Shinya Sato1Chikako Nagasato2Taizo Motomura3Shujiro Okuda4Masahiro Kasahara5Fumio Takahashi6Shinya Yoshikawa7Faculty of Marine Science and Technology, Fukui Prefectural UniversityFaculty of Marine Science and Technology, Fukui Prefectural UniversityField Science Center for Northern Biosphere, Muroran Marine Station, Hokkaido UniversityField Science Center for Northern Biosphere, Muroran Marine Station, Hokkaido UniversityGraduate School of Medical and Dental Science, Niigata UniversityGraduate School of Life Sciences, Ritsumeikan UniversityGraduate School of Life Sciences, Ritsumeikan UniversityFaculty of Marine Science and Technology, Fukui Prefectural UniversityAbstract Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific histone, are involved in chromatin condensation of the sperm nucleus. Among brown algae, sperm of the oogamous Fucales algae have a condensed nucleus. However, the existence of sperm-specific SNBPs in Fucales algae was unclear. Here, we identified linker histone (histone H1) proteins in the sperm and analyzed changes in their gene expression pattern during spermatogenesis in Sargassum horneri. A search of transcriptomic data for histone H1 genes in showed six histone H1 genes, which we named ShH1.1a, ShH1b, ShH1.2, ShH1.3, ShH1.4, and ShH1.5. Analysis of SNBPs using SDS-PAGE and LC–MS/MS showed that sperm nuclei contain histone ShH1.2, ShH1.3, and ShH1.4 in addition to core histones. Both ShH1.2 and ShH1.3 genes were expressed in the vegetative thallus and the male and female receptacles (the organs producing antheridium or oogonium). Meanwhile, the ShH1.4 gene was expressed in the male receptacle but not in the vegetative thallus and female receptacles. From these results, ShH1.4 may be a sperm-specific histone H1 of S. horneri.https://doi.org/10.1038/s41598-024-53729-2 |
| spellingShingle | Yu Takeuchi Shinya Sato Chikako Nagasato Taizo Motomura Shujiro Okuda Masahiro Kasahara Fumio Takahashi Shinya Yoshikawa Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri Scientific Reports |
| title | Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri |
| title_full | Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri |
| title_fullStr | Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri |
| title_full_unstemmed | Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri |
| title_short | Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri |
| title_sort | sperm specific histone h1 in highly condensed sperm nucleus of sargassum horneri |
| url | https://doi.org/10.1038/s41598-024-53729-2 |
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