Spectrophotometric determination of chloramphenicol (after reduction) by nitrogen and conjugation using fluorochlosinol reagent - application to eye drops
<strong>ABSTRACT</strong><br /> A spectrophotometric method for the assay of micro amounts of chloramphenicol was developed. This method is based on the reduction of the nitro-group of chloramphenicol to amino-group, followed by diazotization and coupling with phloroglucin...
| Main Authors: | , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
College of Education for Pure Sciences
2013-12-01
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| Series: | مجلة التربية والعلم |
| Subjects: | |
| Online Access: | https://edusj.mosuljournals.com/article_163096_50e5f03c6a3fbc335d21ecd3cf2bfa60.pdf |
| Summary: | <strong>ABSTRACT</strong><br /> A spectrophotometric method for the assay of micro amounts of chloramphenicol was developed. This method is based on the reduction of the nitro-group of chloramphenicol to amino-group, followed by diazotization and coupling with phloroglucinol in alkaline medium to form a stable yellow azo dye which shows maximum absorption at 419 nm. Beer's law is obeyed over the concentration range (0.4 – 40) ppm. The molar absorptivity is (1.5437×10<sup>4</sup>) l.mol<sup>-1</sup>.cm<sup>-1 </sup>and Sandell sensitivity index of (0.021) µg / cm<sup>2</sup>with a relative error of -1.8 to -0.52% and a relative standard deviation of ± 4.0 to ± 0.69%. The propose method has been applied to the determination of chloramphenicol in eye drop. |
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| ISSN: | 1812-125X 2664-2530 |