Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice
By the experiments of in vivo intranasal infection of 8-10-days-old outbread ICR mice with Monkeypox virus (MPV) in a dose equal 3.83 lg FFU/specimen, investigated was dynamics of the virus accumulation within various organs, blood cells, and blood serum. In 2 days after infection MPV was detected i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2015-12-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/272 |
| _version_ | 1826922364852502528 |
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| author | Al. A. Sergeev A. S. Kabanov L. E. Bulychev O. V. P’Yankov Ar. A. Sergeev S. A. Bodnev D. O. Gorbatovskaya A. S. Zamedyanskaya L. N. Shishkina A. P. Agafonov A. N. Sergeev |
| author_facet | Al. A. Sergeev A. S. Kabanov L. E. Bulychev O. V. P’Yankov Ar. A. Sergeev S. A. Bodnev D. O. Gorbatovskaya A. S. Zamedyanskaya L. N. Shishkina A. P. Agafonov A. N. Sergeev |
| author_sort | Al. A. Sergeev |
| collection | DOAJ |
| description | By the experiments of in vivo intranasal infection of 8-10-days-old outbread ICR mice with Monkeypox virus (MPV) in a dose equal 3.83 lg FFU/specimen, investigated was dynamics of the virus accumulation within various organs, blood cells, and blood serum. In 2 days after infection MPV was detected in blood cells, nasal cavity, lungs, spleen, and duodenum, and in 5 days after - in brain, trachea, liver, kidneys, and blood serum. It was established that 7 days after infection the highest level of MPV production was in the lungs, nasal cavity, and brain, where virus titers in 5 % homogenates were (5.7±0.1), (5.5±0.1), and (5.3±0.3) lg FFU/ml, respectively. In the blood cells virus was traced in 2, 5, and 7 days after challenge, while in blood serum - in 5 and 7 days. MPV blood transfer to the secondary target organs (liver, spleen, duodenum, kidneys, et al. ) was operational, probably, due to the virus proliferation in blood corpuscles. The data obtained and the worked out scheme of MPV dissemination in an organism can be used for the selection and construction of therapeutic anti-pox virus preparations with precise targeted drug delivery. |
| first_indexed | 2024-03-08T13:43:15Z |
| format | Article |
| id | doaj.art-31647faedd1e46d4a264605086d2cff7 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2025-02-17T14:08:40Z |
| publishDate | 2015-12-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-31647faedd1e46d4a264605086d2cff72024-12-24T12:09:52ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2015-12-0104869010.21055/0370-1069-2015-4-86-90272Monkeypox Virus Dissemination in Case of Intranasal Infection of MiceAl. A. Sergeev0A. S. Kabanov1L. E. Bulychev2O. V. P’Yankov3Ar. A. Sergeev4S. A. Bodnev5D. O. Gorbatovskaya6A. S. Zamedyanskaya7L. N. Shishkina8A. P. Agafonov9A. N. Sergeev10State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”State Research Centre of Virology and Biotechnology “Vector”By the experiments of in vivo intranasal infection of 8-10-days-old outbread ICR mice with Monkeypox virus (MPV) in a dose equal 3.83 lg FFU/specimen, investigated was dynamics of the virus accumulation within various organs, blood cells, and blood serum. In 2 days after infection MPV was detected in blood cells, nasal cavity, lungs, spleen, and duodenum, and in 5 days after - in brain, trachea, liver, kidneys, and blood serum. It was established that 7 days after infection the highest level of MPV production was in the lungs, nasal cavity, and brain, where virus titers in 5 % homogenates were (5.7±0.1), (5.5±0.1), and (5.3±0.3) lg FFU/ml, respectively. In the blood cells virus was traced in 2, 5, and 7 days after challenge, while in blood serum - in 5 and 7 days. MPV blood transfer to the secondary target organs (liver, spleen, duodenum, kidneys, et al. ) was operational, probably, due to the virus proliferation in blood corpuscles. The data obtained and the worked out scheme of MPV dissemination in an organism can be used for the selection and construction of therapeutic anti-pox virus preparations with precise targeted drug delivery.https://journal.microbe.ru/jour/article/view/272вирус оспы обезьянаутбредные мышиинтраназальное инфицированиединамика накоплениядиссеминацияmonkeypox virusoutbread miceintranasal infectiondynamics of accumulationdissemination |
| spellingShingle | Al. A. Sergeev A. S. Kabanov L. E. Bulychev O. V. P’Yankov Ar. A. Sergeev S. A. Bodnev D. O. Gorbatovskaya A. S. Zamedyanskaya L. N. Shishkina A. P. Agafonov A. N. Sergeev Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice Проблемы особо опасных инфекций вирус оспы обезьян аутбредные мыши интраназальное инфицирование динамика накопления диссеминация monkeypox virus outbread mice intranasal infection dynamics of accumulation dissemination |
| title | Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice |
| title_full | Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice |
| title_fullStr | Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice |
| title_full_unstemmed | Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice |
| title_short | Monkeypox Virus Dissemination in Case of Intranasal Infection of Mice |
| title_sort | monkeypox virus dissemination in case of intranasal infection of mice |
| topic | вирус оспы обезьян аутбредные мыши интраназальное инфицирование динамика накопления диссеминация monkeypox virus outbread mice intranasal infection dynamics of accumulation dissemination |
| url | https://journal.microbe.ru/jour/article/view/272 |
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