HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis
Abstract Background Long non-coding RNAs (lncRNAs) are promising therapeutic molecules of cancer. Here we aim to study the therapeutic effect and mechanism of a lncRNA, HOXA11-AS, in oral squamous cell carcinoma (OSCC). Methods OSCC tissues and adjacent matched paraneoplastic normal tissues used in...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-019-0838-6 |
| _version_ | 1818280635703230464 |
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| author | Baojun Li Wei Wang Susheng Miao Guofu Li Yuanjing Lv Cheng Xiang Rong Pei |
| author_facet | Baojun Li Wei Wang Susheng Miao Guofu Li Yuanjing Lv Cheng Xiang Rong Pei |
| author_sort | Baojun Li |
| collection | DOAJ |
| description | Abstract Background Long non-coding RNAs (lncRNAs) are promising therapeutic molecules of cancer. Here we aim to study the therapeutic effect and mechanism of a lncRNA, HOXA11-AS, in oral squamous cell carcinoma (OSCC). Methods OSCC tissues and adjacent matched paraneoplastic normal tissues used in this study were collected from 42 OSCC patients. The significant downregulation or upregulation of HOXA11-AS expression in OSCC cells was confirmed by quantitative real-time PCR (qRT-PCR). Bioinformatics analysis of StarBase were performed to investigate the potential microRNAs mediated by HOXA11-AS. HOXA11-AS-transfected cells or control cells were subcutaneously injected into nude mice to further determine the effects of HOXA11-AS on OSCC progression in vivo. Results qRT-PCR analysis indicated that HOXA11-AS expression was significantly upregulated in OSCC tissues. Functional studies revealed that HOXA11-AS significantly promotes cell proliferation, reduces the percentage of G0/G1 phase cells and enhances the cell invasion in OSCC. Bioinformatics analysis suggested that a microRNA (miRNA), miR-518a-3p, is as a target of HOXA11-AS. Alteration of miR-518a-3p levels by HOXA11-AS transduced to changes in PDK1 expression. In a mouse model of OSCC, HOXA11-AS overexpression promoted tumor growth, concomitant with reduced miR-518a-3p expression and increased PDK1 expression. Conclusion Taken together, our study demonstrates that HOXA11-AS/miR-518a-3p/PDK1 axis is an important regulator of OSCC progression and may serve as a potential therapeutic target in OSCC. HARMU20150128, registered at Jan, 28 2018. |
| first_indexed | 2024-12-12T23:52:22Z |
| format | Article |
| id | doaj.art-3164a265e08249879ded9a46a8135add |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-12T23:52:22Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-3164a265e08249879ded9a46a8135add2022-12-22T00:06:40ZengBMCCancer Cell International1475-28672019-05-0119111210.1186/s12935-019-0838-6HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axisBaojun Li0Wei Wang1Susheng Miao2Guofu Li3Yuanjing Lv4Cheng Xiang5Rong Pei6Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, Harbin Medical UniversityDepartment of Oral Maxillofacial Surgery, The First Affiliated Hospital, Harbin Medical UniversityDepartment of Head and Neck Surgery, Harbin Medical University Cancer Hospital, Harbin Medical UniversityDepartment of Neurosurgery, Harbin Medical University Cancer Hospital, Harbin Medical UniversityDepartment of Head and Neck Surgery, Harbin Medical University Cancer Hospital, Harbin Medical UniversityDepartment of Head and Neck Surgery, Harbin Medical University Cancer Hospital, Harbin Medical UniversityDepartment of Head and Neck Surgery, Harbin Medical University Cancer Hospital, Harbin Medical UniversityAbstract Background Long non-coding RNAs (lncRNAs) are promising therapeutic molecules of cancer. Here we aim to study the therapeutic effect and mechanism of a lncRNA, HOXA11-AS, in oral squamous cell carcinoma (OSCC). Methods OSCC tissues and adjacent matched paraneoplastic normal tissues used in this study were collected from 42 OSCC patients. The significant downregulation or upregulation of HOXA11-AS expression in OSCC cells was confirmed by quantitative real-time PCR (qRT-PCR). Bioinformatics analysis of StarBase were performed to investigate the potential microRNAs mediated by HOXA11-AS. HOXA11-AS-transfected cells or control cells were subcutaneously injected into nude mice to further determine the effects of HOXA11-AS on OSCC progression in vivo. Results qRT-PCR analysis indicated that HOXA11-AS expression was significantly upregulated in OSCC tissues. Functional studies revealed that HOXA11-AS significantly promotes cell proliferation, reduces the percentage of G0/G1 phase cells and enhances the cell invasion in OSCC. Bioinformatics analysis suggested that a microRNA (miRNA), miR-518a-3p, is as a target of HOXA11-AS. Alteration of miR-518a-3p levels by HOXA11-AS transduced to changes in PDK1 expression. In a mouse model of OSCC, HOXA11-AS overexpression promoted tumor growth, concomitant with reduced miR-518a-3p expression and increased PDK1 expression. Conclusion Taken together, our study demonstrates that HOXA11-AS/miR-518a-3p/PDK1 axis is an important regulator of OSCC progression and may serve as a potential therapeutic target in OSCC. HARMU20150128, registered at Jan, 28 2018.http://link.springer.com/article/10.1186/s12935-019-0838-6Oral squamous cell carcinomaHOXA11-ASmiR-518a-3pPDK1 |
| spellingShingle | Baojun Li Wei Wang Susheng Miao Guofu Li Yuanjing Lv Cheng Xiang Rong Pei HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis Cancer Cell International Oral squamous cell carcinoma HOXA11-AS miR-518a-3p PDK1 |
| title | HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis |
| title_full | HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis |
| title_fullStr | HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis |
| title_full_unstemmed | HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis |
| title_short | HOXA11-AS promotes the progression of oral squamous cell carcinoma by targeting the miR-518a-3p/PDK1 axis |
| title_sort | hoxa11 as promotes the progression of oral squamous cell carcinoma by targeting the mir 518a 3p pdk1 axis |
| topic | Oral squamous cell carcinoma HOXA11-AS miR-518a-3p PDK1 |
| url | http://link.springer.com/article/10.1186/s12935-019-0838-6 |
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