Leucine supplementation improves leptin sensitivity in high-fat diet fed rats
Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear. Design: Forty-eight male Sprague-Dawley rats were fed with either a hig...
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| Format: | Article |
| Language: | English |
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Swedish Nutrition Foundation
2015-06-01
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| Series: | Food & Nutrition Research |
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| Online Access: | http://www.foodandnutritionresearch.net/index.php/fnr/article/view/27373/40924 |
| _version_ | 1818365504589398016 |
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| author | Xue-Wei Yuan Shu-Fen Han Jian-Wei Zhang Jia-Ying Xu Li-Qiang Qin |
| author_facet | Xue-Wei Yuan Shu-Fen Han Jian-Wei Zhang Jia-Ying Xu Li-Qiang Qin |
| author_sort | Xue-Wei Yuan |
| collection | DOAJ |
| description | Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear. Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot. Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group. Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling. |
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| format | Article |
| id | doaj.art-3165e53d4a4e43d99d6668a1ada404ad |
| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-12-13T22:21:19Z |
| publishDate | 2015-06-01 |
| publisher | Swedish Nutrition Foundation |
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| series | Food & Nutrition Research |
| spelling | doaj.art-3165e53d4a4e43d99d6668a1ada404ad2022-12-21T23:29:22ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2015-06-015901610.3402/fnr.v59.2737327373Leucine supplementation improves leptin sensitivity in high-fat diet fed ratsXue-Wei Yuan0Shu-Fen Han1Jian-Wei Zhang2Jia-Ying Xu3Li-Qiang Qin4 Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China Key Laboratory of Radiation Biology, School of Radiation Medicine and Protection, Soochow University, Suzhou, China Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, ChinaBackground: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear. Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot. Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group. Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.http://www.foodandnutritionresearch.net/index.php/fnr/article/view/27373/40924leptin sensitivityleptin signalingleucinehigh-fat diet |
| spellingShingle | Xue-Wei Yuan Shu-Fen Han Jian-Wei Zhang Jia-Ying Xu Li-Qiang Qin Leucine supplementation improves leptin sensitivity in high-fat diet fed rats Food & Nutrition Research leptin sensitivity leptin signaling leucine high-fat diet |
| title | Leucine supplementation improves leptin sensitivity in high-fat diet fed rats |
| title_full | Leucine supplementation improves leptin sensitivity in high-fat diet fed rats |
| title_fullStr | Leucine supplementation improves leptin sensitivity in high-fat diet fed rats |
| title_full_unstemmed | Leucine supplementation improves leptin sensitivity in high-fat diet fed rats |
| title_short | Leucine supplementation improves leptin sensitivity in high-fat diet fed rats |
| title_sort | leucine supplementation improves leptin sensitivity in high fat diet fed rats |
| topic | leptin sensitivity leptin signaling leucine high-fat diet |
| url | http://www.foodandnutritionresearch.net/index.php/fnr/article/view/27373/40924 |
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