In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer
Recent progress in breast cancer research has led to the identification of Vascular Cell Adhesion Molecule-1 (VCAM-1) as a key actor of metastatic colonization. VCAM-1 promotes lung-metastases and is associated with clinical early recurrence and poor outcome in triple negative breast cancer (TNBC)....
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| Format: | Article |
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MDPI AG
2019-07-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/11/7/1039 |
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| author | Christopher Montemagno Laurent Dumas Pierre Cavaillès Mitra Ahmadi Sandrine Bacot Marlène Debiossat Audrey Soubies Loic Djaïleb Julien Leenhardt Nicolas De Leiris Maeva Dufies Gilles Pagès Sophie Hernot Nick Devoogdt Pascale Perret Laurent Riou Daniel Fagret Catherine Ghezzi Alexis Broisat |
| author_facet | Christopher Montemagno Laurent Dumas Pierre Cavaillès Mitra Ahmadi Sandrine Bacot Marlène Debiossat Audrey Soubies Loic Djaïleb Julien Leenhardt Nicolas De Leiris Maeva Dufies Gilles Pagès Sophie Hernot Nick Devoogdt Pascale Perret Laurent Riou Daniel Fagret Catherine Ghezzi Alexis Broisat |
| author_sort | Christopher Montemagno |
| collection | DOAJ |
| description | Recent progress in breast cancer research has led to the identification of Vascular Cell Adhesion Molecule-1 (VCAM-1) as a key actor of metastatic colonization. VCAM-1 promotes lung-metastases and is associated with clinical early recurrence and poor outcome in triple negative breast cancer (TNBC). Our objective was to perform the in vivo imaging of VCAM-1 in mice models of TNBC. The Cancer Genomic Atlas (TCGA) database was analyzed to evaluate the prognostic role of VCAM-1 in TNBC. MDA-MB-231 (VCAM-1+) and control HCC70 (VCAM-1-) TNBC cells were subcutaneously xenografted in mice and VCAM-1 expression was assessed in vivo by single-photon emission computed tomography (SPECT) imaging using <sup>99m</sup>Tc-cAbVCAM1-5. Then, MDA-MB-231 cells were intravenously injected in mice and VCAM-1 expression in lung metastasis was assessed by SPECT imaging after 8 weeks. TCGA analysis showed that VCAM-1 is associated with a poor prognosis in TNBC patients. In subcutaneous tumor models, <sup>99m</sup>Tc-cAbVCAM1-5 uptake was 2-fold higher in MDA-MB-231 than in HCC70 (<i>p</i> < 0.01), and 4-fold higher than that of the irrelevant control (<i>p</i> < 0.01). Moreover, <sup>99m</sup>Tc-cAbVCAM1-5 uptake in MDA-MB-231 lung metastases was also higher than that of <sup>99m</sup>Tc-Ctl (<i>p</i> < 0.05). <sup>99m</sup>Tc-cAbVCAM1-5 is therefore a suitable tool to evaluate the role of VCAM-1 as a marker of tumor aggressiveness of TNBC. |
| first_indexed | 2024-03-12T19:52:48Z |
| format | Article |
| id | doaj.art-316631da40764dd2a869944555e4ef51 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T19:52:48Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-316631da40764dd2a869944555e4ef512023-08-02T03:00:04ZengMDPI AGCancers2072-66942019-07-01117103910.3390/cancers11071039cancers11071039In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast CancerChristopher Montemagno0Laurent Dumas1Pierre Cavaillès2Mitra Ahmadi3Sandrine Bacot4Marlène Debiossat5Audrey Soubies6Loic Djaïleb7Julien Leenhardt8Nicolas De Leiris9Maeva Dufies10Gilles Pagès11Sophie Hernot12Nick Devoogdt13Pascale Perret14Laurent Riou15Daniel Fagret16Catherine Ghezzi17Alexis Broisat18Laboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceNatural Barriers and Infectiosity, Universite Grenoble Alpes, CNRS, CHU Grenoble Alpes, TIMC-IMAG, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceBiomedical Department, Centre Scientifique de Monaco, 980000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 980000 Monaco, MonacoLaboratory of In Vivo Cellular and Molecular Imaging, ICMI-BEFY, Vrije Universiteit Brussel, Laarbeeklan 103, B-1090 Brussels, BelgiumLaboratory of In Vivo Cellular and Molecular Imaging, ICMI-BEFY, Vrije Universiteit Brussel, Laarbeeklan 103, B-1090 Brussels, BelgiumLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceLaboratory of Bioclinical Radiopharmaceutics, Universite Grenoble Alpes, Inserm, CHU Grenoble Alpes, LRB, 38000 Grenoble, FranceRecent progress in breast cancer research has led to the identification of Vascular Cell Adhesion Molecule-1 (VCAM-1) as a key actor of metastatic colonization. VCAM-1 promotes lung-metastases and is associated with clinical early recurrence and poor outcome in triple negative breast cancer (TNBC). Our objective was to perform the in vivo imaging of VCAM-1 in mice models of TNBC. The Cancer Genomic Atlas (TCGA) database was analyzed to evaluate the prognostic role of VCAM-1 in TNBC. MDA-MB-231 (VCAM-1+) and control HCC70 (VCAM-1-) TNBC cells were subcutaneously xenografted in mice and VCAM-1 expression was assessed in vivo by single-photon emission computed tomography (SPECT) imaging using <sup>99m</sup>Tc-cAbVCAM1-5. Then, MDA-MB-231 cells were intravenously injected in mice and VCAM-1 expression in lung metastasis was assessed by SPECT imaging after 8 weeks. TCGA analysis showed that VCAM-1 is associated with a poor prognosis in TNBC patients. In subcutaneous tumor models, <sup>99m</sup>Tc-cAbVCAM1-5 uptake was 2-fold higher in MDA-MB-231 than in HCC70 (<i>p</i> < 0.01), and 4-fold higher than that of the irrelevant control (<i>p</i> < 0.01). Moreover, <sup>99m</sup>Tc-cAbVCAM1-5 uptake in MDA-MB-231 lung metastases was also higher than that of <sup>99m</sup>Tc-Ctl (<i>p</i> < 0.05). <sup>99m</sup>Tc-cAbVCAM1-5 is therefore a suitable tool to evaluate the role of VCAM-1 as a marker of tumor aggressiveness of TNBC.https://www.mdpi.com/2072-6694/11/7/1039triple negative breast cancerVCAM-1SPECT imagingsdAbs |
| spellingShingle | Christopher Montemagno Laurent Dumas Pierre Cavaillès Mitra Ahmadi Sandrine Bacot Marlène Debiossat Audrey Soubies Loic Djaïleb Julien Leenhardt Nicolas De Leiris Maeva Dufies Gilles Pagès Sophie Hernot Nick Devoogdt Pascale Perret Laurent Riou Daniel Fagret Catherine Ghezzi Alexis Broisat In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer Cancers triple negative breast cancer VCAM-1 SPECT imaging sdAbs |
| title | In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer |
| title_full | In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer |
| title_fullStr | In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer |
| title_full_unstemmed | In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer |
| title_short | In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer |
| title_sort | in vivo assessment of vcam 1 expression by spect ct imaging in mice models of human triple negative breast cancer |
| topic | triple negative breast cancer VCAM-1 SPECT imaging sdAbs |
| url | https://www.mdpi.com/2072-6694/11/7/1039 |
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